12,469 results

1. pTa-HH5-1

   Plasmid

   #124995

   Purpose

   To make transgenic mice for HH5-1 TCRa

   Depositor

   Dan Littman

   Article

   Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

   Insert

   HH5-1 TCRa Tg
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. as-mOPN-PB0

   Plasmid

   #106450

   Purpose

   Bacterial expression of antisense to murine osteopontin

   Depositor

   Georg Weber

   Article

   Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

   Insert

   antisense-osteopontin (Spp1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   antisense

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. JDW 249 (pTol2-Dll4-F2-ETS#2-WT-8X-E1b-EGFP)

   Plasmid

   #156417

   Purpose

   8X copies of the ETS#2 site of the murine Dll4 intronic enhancer and a minimal E1b reporter driving expression of EGFP flanked by Tol2 sites

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   murine Dll4 F2-6/F8 ETS site B/ site #2, 8X
   

   Use

   Zebrafish transgenesis

   Tags

   Expression

   Mutation

   Promoter

   E1b min pro/b-globin intron

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. JDW 295 (pDONR-Dll4-F2-ETS#2-WT-8X)

   Plasmid

   #156412

   Purpose

   Gateway middle entry clone containing an 8X multimerized ETS#2 DNA binding site from the murine Dll4 intronic enhancer

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   murine Dll4 F2- ETS site B/ site #2, 8X
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-S5E1-dTom-nlsdTom

   Plasmid

   #135637

   Purpose

   AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E1 regulatory element

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Insert

   dTom-nlsdTom
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-S5E3-dTom-nlsdTom

   Plasmid

   #135638

   Purpose

   AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E3 regulatory element

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Insert

   dTom-nlsdTom
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-S5E4-dTom-nlsdTom

   Plasmid

   #135639

   Purpose

   AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E4 regulatory element

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Insert

   dTom-nlsdTom
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-S5E7-dTom-nls-dTom

   Plasmid

   #135642

   Purpose

   AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E7 regulatory element

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Insert

   dTom-nlsdTom
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-S5E9-dTom-nls-dTom

   Plasmid

   #135644

   Purpose

   AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E9 regulatory element

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Insert

   dTom-nlsdTom
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-S5E10-dTom-nlsdTom

    Plasmid

    #135645

    Purpose

    AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E10 regulatory element

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Insert

    dTom-nlsdTom
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLCHKO_Ptbp_ex8_1

    Plasmid

    #155081

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLCHKO_Ptbp_ex8_4

    Plasmid

    #155084

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLCHKO_Ptbp_ex8_2

    Plasmid

    #155082

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRDAX-537 C-Tag Venus210[2] L20 Itgb1 mCherry

    Plasmid

    #130016

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    Itgb1 (Itgb1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKLV-gRNA-Bcan-Ntrk1_4

    Plasmid

    #136413

    Purpose

    Lentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP.

    Depositor

    Massimo Squatrito

    Article

    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

    Insert

    U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKLV-gRNA-Myb-Qk_1

    Plasmid

    #136415

    Purpose

    Lentiviral expression of gRNAs targeting intron 4 of murine Qk and intron 9 of murine Myb. Also constitutively expresses Puromycin linked to TagBFP.

    Depositor

    Massimo Squatrito

    Article

    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

    Insert

    U6_sgRNA(Myb)_U6_sgRNA(Qk)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EGFP-Mm_Plk5(300-595) PBD domain

    Plasmid

    #136316

    Purpose

    Mammalian expression of mouse Plk5-D300 (PBD domain 300-595) with N-terminal EGFP

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

    Insert

    PLK5 (Plk5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fragment 300-595

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EGFP-Mm_Plk5(D168A)

    Plasmid

    #136318

    Purpose

    Mammalian expression of mouse Plk5-KD (D168A) with N-terminal EGFP

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

    Insert

    PLK5 (Plk5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D168A

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR-Mm_MyoGEF(WT)

    Plasmid

    #136327

    Purpose

    Mouse MyoGEF-WT in Gateway pENTR

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

    Insert

    PLEKHG6 (Plekhg6 Mouse)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR-Mm_MyoGEF(T575A)

    Plasmid

    #136328

    Purpose

    Mouse MyoGEF-T575A mutant in Gateway pENTR

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

    Insert

    PLEKHG6 (Plekhg6 Mouse)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    T575A

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMAL-C2X dAmp/Kana+-2MT

    Plasmid

    #154353

    Purpose

    Bacterial expression of 2MT

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    2MT
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCas9n-Nanog-R

    Plasmid

    #122303

    Purpose

    Expresses sgRNA targeting mouse Nanog and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Nanog (Nanog Synthetic, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Antares-SEPLuc C1A4E

    Plasmid

    #141088

    Purpose

    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of Nanoluc

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-IRESv24-SEP-C1A4E
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Please see depositor comments

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Anti-ZNF423 [N328B/37R]

    Plasmid

    #140077

    Purpose

    Mammalian Expression Plasmid of anti-ZNF423 (Human). Derived from hybridoma N328B/37.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-ZNF423 (Human) recombinant mouse monoclonal antibody (ZNF423 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Anti-Mad3 [N129A/6R]

    Plasmid

    #140075

    Purpose

    Mammalian Expression Plasmid of anti-Mad3 (Human). Derived from hybridoma N129A/6.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Mad3 (Human) recombinant mouse monoclonal antibody (MXD3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Anti-Kir2.2 K+ channel [N124B/38R]

    Plasmid

    #140074

    Purpose

    Mammalian Expression Plasmid of anti-Kir2.2 K+ channel (Rat). Derived from hybridoma N124B/38.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Kir2.2 K+ channel (Rat) recombinant mouse monoclonal antibody (Kcnj12 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SEPLuc-E2A-cyOFP1

    Plasmid

    #141084

    Purpose

    precursor of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    SEPLuc-E2A-cyOFP1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Anti-Kv1.5 K+ channel [K7/45R]

    Plasmid

    #140069

    Purpose

    Mammalian Expression Plasmid of anti-Kv1.5 K+ channel (Rat). Derived from hybridoma K7/45.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Kv1.5 K+ channel (Rat) recombinant mouse monoclonal antibody (Kcna5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Anti-KCNT1/Slo2.2/Slack K+ channel [N3/26R]

    Plasmid

    #140064

    Purpose

    Mammalian Expression Plasmid of anti-KCNT1/Slo2.2/Slack K+ channel (Rat). Derived from hybridoma N3/26.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-KCNT1/Slo2.2/Slack K+ channel (Rat) recombinant mouse monoclonal antibody (Kcnt1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. sg1

    Plasmid

    #113966

    Purpose

    Single short guide RNA targeting GTATAGCATACATTATACG

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge