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pCR3-vsv-dCEN-Incenp

Plasmid

#108479

Purpose

expression of vsv-dCEN-INCENP

Depositor

Susanne Lens

Article

Vader et al Mol Biol Cell. 2007 Nov;18(11):4553-64.

Insert

dCEN-INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

CEN box removed

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10

Plasmid

#104619

Purpose

Expresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

3xVAS4-hsp70-Syn21-FLAG-Cerulean-P10

Tags

FLAG

Expression

Insect

Promoter

hsp70

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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PatoM-OG241

Plasmid

#72842

Purpose

Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoM

Expression

Bacterial

Promoter

PatoM

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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CDk4-luciferase fragment B

Plasmid

#86659

Purpose

Luciferase reporter containing CDK4 promoter

Depositor

Jaime Modiano

Article

Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

Insert

CDK4 promoter (CDK4 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

generated by cleavage at the KpnI site from 880 (…

Promoter

CDK7

Available Since

June 20, 2017

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF4F07

Plasmid

#71056

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Mi-2 (Mi-2 Fly)

Use

Gateway entry vector

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1G05

Plasmid

#70809

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CkIIbeta (CkIIbeta Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1B07

Plasmid

#70819

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CkIIalpha (CkIIalpha Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pET30Xa-SBL1(160E,NoCys)

Plasmid

#66875

Purpose

Expresses a cysteine-free version of pET30Xa-SBL1(160E) (4Cys to Ser) with N-term Histag

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (S160E) with all Cys to Ser mutations

Tags

Histag

Expression

Bacterial

Mutation

S160E; changed 4 (all) cysteines to serine

Promoter

T7lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pGL2-HKII-F

Plasmid

#64518

Purpose

AS-30D hepatoma hexokinase II 0.075 kbp exon I-luciferase reporter vector

Depositor

Peter Pedersen

Article

Mathupala et al J Biol Chem. 2001 Nov 16;276(46):43407-1

Insert

Type II hexokinase gene, partial cds and promoter region (Hk2 Rat)

Use

Luciferase

Tags

firefly luciferase

Mutation

Isolated from AS-30D rat hepatoma

Promoter

Hexokinase II promoter deleted

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only
3xFN-Tel-TAL/pMXs-neo

Plasmid

#63591

Purpose

Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector with the neomycin resistance gene.

Depositor

Hodaka Fujii

Article

Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038

Insert

3xFVN-Tel-TAL

Use

Retroviral and TALEN

Tags

3xFLAG-tag, V5-tag, and nuclear localization sign…

Expression

Mammalian

Promoter

LTR

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pUASp-Flag-Myc-Qin-Tudor-attB

Plasmid

#44210

Depositor

Phillip Zamore

Article

Zhang et al Mol Cell. 2011 Nov 18;44(4):572-84. doi:

Insert

Qin-Tudor-domain (qin Fly)

Use

Fly transformation

Tags

Flag and Myc

Mutation

lacks E3 domain on N-term (del aa1-274)

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
pGMEH-0X750-luxAB-DAS+4

Plasmid

#49519

Purpose

Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; replicates episomally

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

P750-luxAB-DAS+4

Tags

DAS+4 (AANDENYSENYADAS)

Expression

Bacterial

Promoter

P750

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-Cx43-M100/125L-siResist

Plasmid

#49855

Purpose

Encodes human connexin 43 with M100 and M125 mutated to L and wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Expression

Mammalian

Mutation

Methionine100 and Methionine 125 mutated to Leuci…

Promoter

CMV

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.2-Cx43-M100L-siResist

Plasmid

#49854

Purpose

Encodes human connexin 43 with M100 mutated to L and wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Expression

Mammalian

Mutation

Methionine100 mutated to Leucine. Contains severa…

Promoter

CMV

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only
TRS6E1b-luc(-732/-721) mutant 4

Plasmid

#45387

Depositor

Thomas Gelehrter

Article

Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

Insert

6 copies of hPAI-1 promoter -732/-721 four point mutation (SERPINE1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

mutated from agacaaggttgt to acactaggatga

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro-cfSec15A_2

Plasmid

#26715

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Dog Sec15A RNAi (EXOC6 C. familiaris (dog RNAi))

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Sec15A RNAi.

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro-cfSec15A_5

Plasmid

#26716

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Dog Sec15A RNAi (EXOC6 C. familiaris (dog RNAi))

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Sec15A-specific RNAi.

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
Comp AlphaPS3

Plasmid

#14071

Depositor

Richard Hynes

Article

Stark et al Development. 1997 Nov . 124(22):4583-94.

Insert

Drosphila Comp Alpha PS3 Integrin (scb Fly)

Expression

Bacterial

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
HsCLYBL(aa23-340) D320N-His

Plasmid

#111289

Purpose

bacterial expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

human CLYBL ( aa23-340) D320N codon optimized for bacterial expression (CLYBL Human)

Tags

His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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pHR-TetOn-mCherry-OAZ1_FS-YFP

Plasmid

#232356

Purpose

Mammalian expression of dox-inducible polyamine sensor (polyamine levels = YFP/Cherry)

Depositor

Ankur Jain

Article

Sharma et al bioRxiv [Preprint]. 2024 Nov 17:2024.08.

Insert

OAZ1 derived polyamine sensing module (OAZ1 Human)

Use

Lentiviral

Tags

eYFP and mCherry

Expression

Mammalian

Promoter

TRE3G

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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