We narrowed to 10,431 results for: plasmids 101

1. sgRNA BRCA1 T922I

   Plasmid

   #139328

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA1 T922I using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA1 T922I using base editing
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. B-HeFSpCas9

   Plasmid

   #126766

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-HeFSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HeFSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-HeFSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A; R661A; Q695A; K848A; Q926A; K1003A; R1060A…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPRIME-CMV-GFP-shDyn2

   Plasmid

   #132712

   Purpose

   Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Pdyn gene

   Depositor

   Robert Messing

   Article

   Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

   Insert

   shDyn(2) (Pdyn Rat)
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Oct. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-BASIC- ARHGAP29 Mutant PROMOTER

   Plasmid

   #104156

   Purpose

   luciferase reporter with ARHGAP29 mutant promoter

   Depositor

   Marius Sudol

   Article

   Qiao et al Cell Rep. 2017 May 23;19(8):1495-1502. d

   Insert

   ARHGAP29 promoter (ARHGAP29 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   delete two TEAD binding sites

   Promoter

   ARHGAP29

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPRIME-CMV-GFP-shDyn1

   Plasmid

   #132711

   Purpose

   Encodes short hairpin RNA (shRNA) #1 that targets the 3’-untranslated region of the rat Pdyn gene

   Depositor

   Robert Messing

   Article

   Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

   Insert

   shDyn(1) (Pdyn Rat)
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Oct. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. B-eSpCas9

   Plasmid

   #126761

   Purpose

   Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A; K1003A; R1060A; amino acids 1005-1013 repl…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. B-evoSpCas9

   Plasmid

   #126765

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-evoSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   M495V; Y515N; K526E; R661Q; amino acids 1005-1013…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. U6_sgRNA_CAG_hSt1Cas9_CNRZ1066

   Plasmid

   #136651

   Purpose

   A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:CNRZ1066 chimera) and its U6-driven sgRNA.

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9:CNRZ_1066 Chimera
   sgRNA for St1Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG and hU6

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMTgR2

   Plasmid

   #107286

   Purpose

   Plasmid can be used for Drosophila S2 driven secretion of extracellular part of human interferon-_ receptor 2.

   Depositor

   Bohdan Schneider

   Article

   Mikulecky et al Acta Crystallogr D Struct Biol. 2016 Sep

   Insert

   Interferon gamma receptor 2 (IFNGR2 Human)
   

   Tags

   CATCATCACCATCACCATGA

   Expression

   Insect

   Promoter

   Metallothionein promoter

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV- GfaABC1D::Cas9-HA-sgRNA-Non-Targeting

    Plasmid

    #241026

    Purpose

    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of a non-targeting sgRNA; used as control for SaCas9 based knock-outs in murine astrocytes

    Depositor

    Mark Verheijen

    Article

    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23

    Insert

    U6 driven empty sgRNA
    

    Use

    AAV and CRISPR

    Tags

    SaCas9 + 3X HA-Tag

    Expression

    Mammalian

    Promoter

    GfaABC1D

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE2-TetO-SOX17E57A

    Plasmid

    #216177

    Purpose

    Generate lentiviruses encoding for human SOX17E57A (HMG box numbering); for use in pluripotency reprogramming

    Depositor

    Ralf Jauch

    Article

    Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

    Insert

    SOX17 (SOX17 Human)
    

    Use

    Lentiviral

    Mutation

    Glu113Glu (silent mutation); Glu120Glu (silent mu…

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE2-TetO-SOX17E57C

    Plasmid

    #216178

    Purpose

    Generate lentiviruses encoding for human SOX17E57C (HMG box numbering); for use in pluripotency reprogramming

    Depositor

    Ralf Jauch

    Article

    Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

    Insert

    SOX17 (SOX17 Human)
    

    Use

    Lentiviral

    Mutation

    Glu113Glu (silent mutation); Glu120Glu (silent mu…

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHAGE2-TetO-SOX17E57D

    Plasmid

    #216179

    Purpose

    Generate lentiviruses encoding for human SOX17E57D (HMG box numbering); for use in pluripotency reprogramming

    Depositor

    Ralf Jauch

    Article

    Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

    Insert

    SOX17 (SOX17 Human)
    

    Use

    Lentiviral

    Mutation

    Glu113Glu (silent mutation); Glu120Glu (silent mu…

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHAGE2-TetO-SOX17E57G

    Plasmid

    #216181

    Purpose

    Generate lentiviruses encoding for human SOX17E57G (HMG box numbering); for use in pluripotency reprogramming

    Depositor

    Ralf Jauch

    Article

    Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

    Insert

    SOX17 (SOX17 Human)
    

    Use

    Lentiviral

    Mutation

    Glu113Glu (silent mutation); Glu120Glu (silent mu…

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHAGE2-TetO-SOX17E57H

    Plasmid

    #216182

    Purpose

    Generate lentiviruses encoding for human SOX17E57H (HMG box numbering); for use in pluripotency reprogramming

    Depositor

    Ralf Jauch

    Article

    Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

    Insert

    SOX17 (SOX17 Human)
    

    Use

    Lentiviral

    Mutation

    Glu113Glu (silent mutation); Glu120Glu (silent mu…

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE2-TetO-SOX17E57I

    Plasmid

    #216183

    Purpose

    Generate lentiviruses encoding for human SOX17E57I (HMG box numbering); for use in pluripotency reprogramming

    Depositor

    Ralf Jauch

    Article

    Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

    Insert

    SOX17 (SOX17 Human)
    

    Use

    Lentiviral

    Mutation

    Glu113Glu (silent mutation); Glu120Glu (silent mu…

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-DIO- alpha-MSH

    Plasmid

    #239044

    Purpose

    Overexpression of alpha-MSH

    Depositor

    Qingchun Tong

    Article

    Xu et al Cell Rep. 2023 May 30;42(5):112502. doi:

    Insert

    alpha-MSH (Pomc Mouse)
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-DIO-beta-Endorphin

    Plasmid

    #239043

    Purpose

    Overexpression of beta endorphin

    Depositor

    Qingchun Tong

    Article

    Xu et al Cell Rep. 2023 May 30;42(5):112502. doi:

    Insert

    beta-Endorphin (Pomc Mouse)
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV PHP.eB-CAG-Venus-P2A-Tau-RING

    Plasmid

    #233696

    Purpose

    AAV expression of Venus-P2A-Tau-RING from CAG promoter

    Depositor

    Leo James

    Article

    Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

    Insert

    Venus-P2A-Tau-RING
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV 9P31-hSyn-Venus-P2A-Tau-RING

    Plasmid

    #233693

    Purpose

    AAV expression of Venus-P2A-Tau-RING from hSyn promoter

    Depositor

    Leo James

    Article

    Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

    Insert

    Venus-P2A-Tau-RING
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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