We narrowed to 13,184 results for: BASE
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Plasmid#172838PurposeMammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
ABEMax nSaCas9
Plasmid#135364PurposeExpresion of SaCas9 nickase (D10A) with ABEMax at the N terminusDepositorInsertABEMax nSaCas9
UseCRISPRExpressionMammalianMutation(V82G)Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nCas9-UGI control for Target-AID (pRZ1047)
Plasmid#131299PurposeCAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).DepositorInsertNLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP
ExpressionMammalianPromoterCAGAvailable SinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-HF2-BE2
Plasmid#120396PurposeExpresses HF2-BE2 in mammalian cellsDepositorInsertHF2-BE2
ExpressionMammalianMutationMammalian codon-optimized Cas9-HFPromoterCMVAvailable SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN_TmiR_G13-L_G12-E
Plasmid#25763PurposeEntry vector with TRE promoter driving both mouse G alpha 12 and G alpha 13 miR30-based shRNAs.DepositorInsertG alpha 13 and 12 miR-shRNA
UseGateway CloningAvailable SinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
enIscB
Plasmid#205410PurposeVector encoding human codon-optimized enhanced-activity OgeuIscB driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoterDepositorInsertpCAG_Flag_SV40NLS_OgeuIscB*_npNLS_polyA_pU6__RNA*_pCMV_mCherry
ExpressionMammalianMutationE85R+H369R+S387R+S457RPromoterCAG, hU6, CMVAvailable SinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-MjDim1
Plasmid#189936PurposeExpression of Mjdim1, a methyltransferase used in m6A-SAC-seq.DepositorInsertrsmA (MJ_RS00455 Methanocaldococcus jannaschii)
Tags6x His tag, FLAG tag, SUMO tagExpressionBacterialPromoterT7 promoterAvailable SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
PEA1-Puro-HEK3 CTT ins
Plasmid#171996PurposeDelivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmidDepositorInsertCbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
ExpressionMammalianPromoterCMV for Cas9, U6 for gRNAsAvailable SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK-caCas9-NatMX-Neut5L
Plasmid#89576PurposecaCas9 integrating vector into the Neut5L locusDepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailable SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAYC
Plasmid#62657PurposeMammalian expression of iCre recombinase from the broadly active CAG promoter/enhancerDepositorInsertiCre
ExpressionMammalianAvailable SinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only