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pISH-Gucy1b2-probe2

Plasmid

#105455

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. d

Insert

Gucy1b2 (Gucy1b2 Mouse)

Use

To ganerate riboprobe

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Trpc2-probe1

Plasmid

#105473

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Cell Rep. 2014 Jul 24;8(2):583-95. doi:

Insert

Trpc2 (Trpc2 Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pIRES:mTREK-1(K271Q)

Plasmid

#133273

Purpose

Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tag

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Tags

HA

Expression

Mammalian and Yeast

Mutation

K271Q

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pHybr_r2a>mG3-srt-his

Plasmid

#124805

Purpose

HDR template to knock-in mouse IgG3 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG3 producing cell lines.

Depositor

Ferenc Scheeren, Martijn Verdoes

Article

van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

Insert

Murine IgG3

Use

CRISPR; Hdr template

Tags

Histag (HHHHHH) and Sortag (LPETGG)

Expression

Bacterial and Mammalian

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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P3-IRES-tauGFP LNL TV

Plasmid

#15643

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

P3-IRES-tauGFP LNL TV

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
RhoJ

Plasmid

#55696

Purpose

expression of mouse RhoJ

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoJ (Rhoj Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pBluescript-mHMGB4

Plasmid

#31620

Depositor

Yasuhiko Kawakami, Martin Lotz

Article

Itou et al Dev Dyn. 2011 May . 240(5):1151-62.

Insert

HMGB4 (Hmgb4 Mouse)

Expression

Bacterial

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
RND1

Plasmid

#55701

Purpose

expression of mouse RND1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RND1 (Rnd1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
fgfprom luc

Plasmid

#69446

Purpose

basal plasmid containing minimal fgf4 promoter used for testing enhancer activity of DNAs inserted immediately upstream of the promoter.

Depositor

Lisa Dailey

Article

Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

Insert

minimal fgf4 promoter

Use

Luciferase

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-NL2CT

Plasmid

#163623

Purpose

mouse NL2 N-terminally tagged with GST

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse NL2 (Nlgn2 Mouse)

Tags

GST

Expression

Mammalian

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBa.Halo-KIF13A tail 361-1749

Plasmid

#162720

Purpose

Mammalian expression of WT mouse Kif13A with deletion of the 360 N-terminal residues

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

Kif 13A (Kif13a Mouse)

Expression

Mammalian

Mutation

WT with deletion of the 360 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-Flag-mPar6a.PIG

Plasmid

#24599

Depositor

Senthil Muthuswamy

Article

Aranda et al Nat Cell Biol. 2006 Nov . 8(11):1235-45.

Insert

Par6c

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
mEmerald-Telethonin-C-18

Plasmid

#54264

Purpose

Localization: Scaffold, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Telethonin

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pB6-Csrp2-T2A-sfGFP-iCre-ERT2-FNF-DTA

Plasmid

#140270

Purpose

B6J Csrp2-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy number

Depositor

Mario Capecchi

Insert

Csrp2 (Csrp2 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

C-terminal fusion of T2A-sfGFP-iCre-ERT2

Expression

Mammalian

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p064 pCS mTRPS1 P1

Plasmid

#11035

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 P1 (Trps1 Mouse)

Use

Xenopus, mammalian, avian, and zebrafish

Mutation

Truncation at aa338 to mimic C338X mutation found…

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only
2xHA MED10 pBacPAK8

Plasmid

#17321

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

MED10 (Med10 Mouse)

Tags

2xHA

Expression

Insect

Available Since

March 11, 2008

Availability

Academic Institutions and Nonprofits only
pCLX-KRT7-GFP

Plasmid

#114312

Purpose

Tissue-specific expression of the GFP living color under the control of the Keratin7 promoter

Depositor

Patrick Salmon

Insert

GFP

Use

Lentiviral

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CRISPR V2 sgShmt2_2

Plasmid

#106315

Purpose

Express Cas9 and sgRNA targeting mShmt2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting mShmt2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA-Δ90AEGFP

Plasmid

#26644

Depositor

Anjen Chenn

Insert

Δ90beta catenin

Tags

EGFP-mut4

Expression

Mammalian

Mutation

Deletion of first 90 aa of beta catenin

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-shDnd1-No7

Plasmid

#70068

Purpose

TRC lentiviral vector for shRNA against mouse Dnd1 #7

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (Dnd1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only