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pFosill-3

Plasmid

#89697

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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mEos4b-Actin-C-18

Plasmid

#57500

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Insert

Actin (ACTB Human)

Tags

mEos4b

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pDONR223_NKX2-5

Plasmid

#221479

Purpose

Gateway cloning of NKX2-5 overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-5 (NKX2-5 Human)

Use

Gateway Cloning

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pFosill-2

Plasmid

#89696

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMal-E4rGel

Plasmid

#51280

Purpose

Expresses a fibronectin fusion with gelonin targeting EGFR in bacteria

Depositor

Dane Wittrup

Article

Pirie et al J Biol Chem. 2011 Feb 11;286(6):4165-72.

Insert

E4rGel

Expression

Bacterial

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pSPB845

Plasmid

#128296

Purpose

asymmetric plasmid partitioning E. coli - regulatory plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

PLlacO1 - lp-cfp-parB, BBa_J23110 - lacI

Expression

Bacterial

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pIC144

Plasmid

#63067

Purpose

Retroviral expression of EGFP-TEV-S-tag CENP-K for protein localization and affinity purification (LAP)

Depositor

Iain Cheeseman

Insert

CENP-K, IMAGE clone 3511065 (CENPK Human)

Use

Retroviral

Tags

EGFP and TEV-S

Expression

Mammalian

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pEB2-TurboRFP

Plasmid

#103996

Purpose

Plasmid encoding TurboRFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

TurboRFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (R32G, T68A, L79F, …

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM60-Tiar

Plasmid

#38244

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Tiar (Tial1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
pJFRC6-15XUAS-IVS-mCD8::GFP

Plasmid

#26219

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

mCD8::GFP

Expression

Insect

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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CUP Cyc8 YFP p316

Plasmid

#15594

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

CYC8 (CYC8 Budding Yeast)

Tags

YFP

Expression

Yeast

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
GST-14-3-3dm (pEBG)

Plasmid

#1943

Depositor

Joseph Avruch

Article

Tzivion et al Nature 1998 Jul 2;394(6688):88-92.

Insert

14-3-3 zeta (Ywhaz Mouse)

Tags

GST

Expression

Mammalian

Mutation

Dimerization-deficient mutant of 14-3-3zeta aa: E…

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pBTK1056

Plasmid

#209546

Purpose

BTK Type 4 antibiotic resistance cassette for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Type 4 Kanamycin resistance cassette

Use

Synthetic Biology

Available Since

Jan. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pUG-124

Plasmid

#104635

Purpose

Level 2 construct

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB_EL4

Tags

HIS-Ubq, HA-E2, GST-E3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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mKeima-Red-Actin-7

Plasmid

#56013

Purpose

Localization: Actin, Excitation: 440, Emission: 620

Depositor

Michael Davidson

Insert

Actin (ACTB Human)

Tags

mKeima-Red

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only
Dronpa-Actin-2

Plasmid

#57257

Purpose

Localization: Actin, Excitation: 503, Emission: 518

Depositor

Michael Davidson

Insert

Actin (ACTB Human)

Tags

Dronpa

Expression

Mammalian

Mutation

Actin lacks start codon

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
CUP Pan1 YFP p316

Plasmid

#15592

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

PAN1 (PAN1 Budding Yeast)

Tags

YFP

Expression

Yeast

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
mEos-A69V-Actin-7

Plasmid

#57334

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

Actin (ACTB Human)

Tags

mEos A69V

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pNR284

Plasmid

#79550

Purpose

Output plasmid: contains 16-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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MIP-T3

Plasmid

#99511

Purpose

to express MIP-T3 in mammalian system

Depositor

Dong-Yan Jin

Article

Ng et al J Immunol. 2011 Dec 15;187(12):6473-82.

Insert

MIP-T3 (TRAF3IP1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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