We narrowed to 10,337 results for: plasmids 101

1. pEX-CFP-STIM1-deltaK

   Plasmid

   #18860

   Depositor

   Tobias Meyer

   Article

   Liou et al Proc Natl Acad Sci U S A. 2007 May 29. 1

   Insert

   STIM1 (STIM1 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Stop codon at amino acid position 671 of STIM1. C…

   Promoter

   Available Since

   Oct. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeo

   Plasmid

   #74919

   Purpose

   Mammalian expression of mKlf2 and mNanog-mCherry

   Depositor

   Jose Silva

   Article

   dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

   Inserts

   Klf2 (Klf2 Mouse)
   Nanog (Nanog Mouse)
   

   Use

   Tags

   T2A and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

3. Coexpressed Free GFP-NES

   Plasmid

   #182491

   Purpose

   Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Inserts

   FPPS
   GFP-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10 and GAL7

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-Kir2.1Mut-2A-mScarlet-KASH

   Plasmid

   #203841

   Purpose

   non-functional Kir2.1(mutant) expression as a control for the silencing of neurons with Kir2.1 and mScarlet-KASH expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   Kir2.1 (Kcnj2 Mouse)
   

   Use

   AAV

   Tags

   2A-mScarlet-KASH

   Expression

   Mutation

   G144A, Y145A, G146A

   Promoter

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV2_hSyn_Aurora_Citrine

   Plasmid

   #98217

   Purpose

   Red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.

   Depositor

   Simon Wiegert

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct Aurora gene
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   V59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…

   Promoter

   human synapsin

   Available Since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV2_hSyn_iChloC_CA_Citrine

   Plasmid

   #98220

   Purpose

   Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.

   Depositor

   Simon Wiegert

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct iChloC_C128A gene
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   E83Q, E90R, E101S, C128A, T159C, D156N

   Promoter

   human synapsin

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(Stat3)-PGKpuroBFP-W

   Plasmid

   #105027

   Purpose

   Lentiviral gRNA plasmid targeting mouse Stat3 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Stat3 (Stat3 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAWp28-v1 scaffold

   Plasmid

   #157976

   Purpose

   pBT264-U6p-{2xBbsI}-v1 sgRNA scaffold-{MfeI} Note: This plasmid is unstable. Screen multiple colonies to identify full length clones.

   Depositor

   Alan Siu Lun Wong

   Article

   Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPRIME-CMV-GFP-shCrh1

   Plasmid

   #132709

   Purpose

   Encodes short hairpin RNA (shRNA) #1 that targets the 3’-untranslated region of the rat Crh gene

   Depositor

   Robert Messing

   Article

   Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

   Insert

   shCrh(1) (Crh Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLL6 sgRNA(MS2)-MS2-hA3a D131E

    Plasmid

    #112132

    Purpose

    empty sgRNA cloning vector with MS2-humanA3a D131E

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    human Apobec3a D131E mutant (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D131E mutant in hA3a

    Promoter

    hU6,CBh

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONOR-SNCAe3-WT

    Plasmid

    #85847

    Purpose

    Donor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomato

    Depositor

    Jens Schwamborn

    Article

    Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

    Insert

    SNCA exon 3 homology arms (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV- GfaABC1D::Cas9-HA-sgRNA-Non-Targeting

    Plasmid

    #241026

    Purpose

    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of a non-targeting sgRNA; used as control for SaCas9 based knock-outs in murine astrocytes

    Depositor

    Mark Verheijen

    Article

    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23

    Insert

    U6 driven empty sgRNA
    

    Use

    AAV and CRISPR

    Tags

    SaCas9 + 3X HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6gRNA5(hEGFR_2b)-PGKpuro2ABFP-W

    Plasmid

    #208432

    Purpose

    Lentiviral gRNA plasmid targeting human EGFR gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Heliyon. 2024 Oct 22;10(21):e39677. doi:

    Insert

    EGFR (EGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    human U6

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKLV2-U6gRNA5(hEGFR_1k)-PGKpuro2ABFP-W

    Plasmid

    #208431

    Purpose

    Lentiviral gRNA plasmid targeting human EGFR gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Heliyon. 2024 Oct 22;10(21):e39677. doi:

    Insert

    EGFR (EGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    human U6

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBiFC-SS-VN155(I152L)-IRE1-dLD

    Plasmid

    #217752

    Purpose

    Expresses the VN fragment of BiFC-IRE1-dLD

    Depositor

    Christina Chan

    Article

    Cho et al Cell Rep. 2019 Apr 2;27(1):307-320.e5. d

    Insert

    Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1 Human)
    

    Use

    Tags

    Venus (1-154, I152L)

    Expression

    Mammalian

    Mutation

    deleted amino acids 29-408

    Promoter

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPBbsr2-NRG1-ScNeo

    Plasmid

    #209911

    Purpose

    To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    NRG1-ScNeo (NRG1 Human)
    

    Use

    Tags

    mNeonGreen and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCSIIpuro-HBEGF-mNeonGreen

    Plasmid

    #209912

    Purpose

    To monitor the expression of HB-EGF, the plasmid encodes a recombinant HB-EGF fused intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    HBEGF-mNeonGreen (HBEGF Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCSIIpuro-EREG-mNeonGreen

    Plasmid

    #209914

    Purpose

    To monitor the expression of Epiregulin, the plasmid encodes a recombinant Epiregulin fused intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    EREG-mNeonGreen (EREG Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBK1300-AAV-EFSNC-dCjCas9-MBD2(176-231)

    Plasmid

    #223145

    Purpose

    Expression of truncated MBD2 with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    MBD2 (MBD2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 176-231

    Promoter

    EF1a

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBK1299-AAV-EFSNC-dCjCas9-MBD1(529-592)

    Plasmid

    #223144

    Purpose

    Expression of truncated MBD1 with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    MBD1 (MBD1 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 529-592

    Promoter

    EF1a

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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