We narrowed to 9,614 results for: Coli

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pAJM.847

Plasmid

#108524

Purpose

PhlFAM + PPhlF-YFP Reporter. DAPG sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PhlFAM + PPhlF-YFP

Expression

Bacterial

Mutation

PhlF: S50G S73I R95H A121V M137I N144D T155I R200C

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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prDNA spacer-LacOx50-20xMS2-Hyg

Plasmid

#210219

Purpose

Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Inserts

50xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET21-Flavidin

Plasmid

#89881

Purpose

Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling

Depositor

Mark Howarth

Article

Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

Insert

Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag

Tags

6 glutamate tag (C terminal on insert)

Expression

Bacterial

Mutation

K80R, K121R, K132R, K134R

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pRXG

Plasmid

#113643

Purpose

This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.

Depositor

Jeffrey Barrick

Article

Monk et al ACS Synth Biol. 2016 Sep 20.

Insert

mRFP1-linker-sfGFP

Use

Synthetic Biology

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pS3

Plasmid

#50596

Purpose

Backbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5

Depositor

Jay Keasling

Article

Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

Inserts

aroE
aroD
aroB
aroG*
ppsA
tktA

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only

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pFRET-HSP33 cys

Plasmid

#16076

Depositor

Ulrich Hammerling

Article

Guzy et al Cell Metab. 2005 Jun;1(6):401-8. doi: 10

Insert

HSP33 cys

Tags

ECFP and EYFP

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pAJM.1642

Plasmid

#108535

Purpose

CinRAM + PCin-YFP Reporter. OHC14 sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

CinRAM + PCin-YFP

Expression

Bacterial

Mutation

CinR: S72C V80I G114D G115D R137H K220R A224T *24…

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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hDHFR

Plasmid

#184707

Purpose

bacterial expression of human DHFR

Depositor

Peter Wright

Article

Bhabha et al FEBS Lett. 2011 Nov 16;585(22):3528-32.

Insert

DHFR (DHFR Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-rssA

Plasmid

#89957

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

rssA gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pLG021 (AVAST type 5)

Plasmid

#157899

Purpose

Expresses the AVAST type 5 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

AVAST type 5 (STAND ATPase with N-terminal SIR2)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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