We narrowed to 16,541 results for: puromycin

1. qTAG-C-moxGFP-Puro-LAMP1

   Plasmid

   #207788

   Purpose

   Donor template for moxGFP-2A-Puro insertion into the C-terminus of the LAMP1 locus. For lysosome visualization.To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LAMP1 Homology Arms flanking a moxGFP-Puro Cassette (LAMP1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Dec. 1, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pLPC-MYC-hTRF1

   Plasmid

   #64164

   Purpose

   Retroviral vector expressing human TRF1 with N-terminal MYC tag

   Depositor

   Titia de Lange

   Article

   Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

   Insert

   hTRF1 (TERF1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cre-responsive Cas9 expressing transposon library vector

   Plasmid

   #164138

   Purpose

   Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6, EF-1a

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF1981

   Plasmid

   #144320

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TBX4 (TBX4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. TVBB C-term-mScarlet-Puro

   Plasmid

   #169220

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Puro at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mScarlet-P2A-Puro
   

   Use

   Targeting vector backbone

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF2027

   Plasmid

   #143994

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NPAS2 (NPAS2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF0831

   Plasmid

   #142208

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZBTB33 (ZBTB33 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. POLD1_pLX307

   Plasmid

   #98358

   Purpose

   Lentiviral expression of POLD1

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   POLD1 (POLD1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   E1Fa

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lentiviral-His-tagged-PSPH

   Plasmid

   #134786

   Purpose

   Stable overexpression lentiviral vector of His tagged PSPH

   Depositor

   Seon-Young Kim

   Article

   Park et al Mol Cells. 2019 Aug 31;42(8):604-616. do

   Insert

   Phosphoserine phosphatase (PSPH Human)
   

   Use

   Lentiviral

   Tags

   His

   Promoter

   CMV

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti HsATP13A3 WT

    Plasmid

    #195849

    Purpose

    Expresses wild-type Homo sapiens ATP13A3

    Depositor

    Veerle Baekelandt

    Article

    Houdou et al Biomolecules. 2023 Feb 9;13(2):337. doi:

    Insert

    ATPase 13A3 (ATP13A3 Human)
    

    Use

    Lentiviral

    Mutation

    D498N, L956P, M850I, V855M, R858H, L675V

    Promoter

    CMV

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF0335

    Plasmid

    #144358

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF91 (ZNF91 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. TVBB C-term-mNeongreen-Puro

    Plasmid

    #169228

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Puro at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mNeongreen-P2A-Puro
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-CDH5-mCitrine-IRES-puro

    Plasmid

    #85143

    Purpose

    lentiviral expression of human VE-cadherin, C-terminally tagged with mCitrine

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    CDH5-mCitrine (VE-cadherin, C-terminally tagged with mCitrine (CDH5 Human)
    

    Use

    Lentiviral

    Tags

    mCitrine

    Promoter

    CAG

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. EGFR D770-N771 insNPG

    Plasmid

    #11016

    Purpose

    Retroviral construct for expressing human EGFR with D770-N771 insNPG mutation in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR D770-N771 insNPG (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    D770-N771 insNPG

    Available Since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pLJM1-TMEM192-FLAG

    Plasmid

    #164977

    Purpose

    Lentiviral vector for TMEM192 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. tet_pLKO.1_puro_shNRF2 #2

    Plasmid

    #136585

    Purpose

    Expresses an inducible short hairpin targeting human NRF2 sequence

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    shNRF2v2 (NFE2L2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. SFFV-DTAG-NTERM-GFP

    Plasmid

    #185760

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, dTAG

    Mutation

    none

    Promoter

    SFFV

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti.CMV/TO_PRKAA2

    Plasmid

    #74447

    Purpose

    Lentiviral expression vector encoding untagged WT human AMPK alpha 2

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    AMPK alpha 2 (PRKAA2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pCW-HA-hRb-delta-CDK-T373D-S608D-S612D-puro

    Plasmid

    #212675

    Purpose

    Express tagged hRb phosphosite mutant

    Depositor

    Yumi Konagaya, Tobias Meyer

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    Rb (RB1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    15 CDK phospho-sites are mutated to alanines, and…

    Promoter

    TRE

    Available Since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSH-EFIRES-P-Seipin-miniIAA7-mScarlet-I

    Plasmid

    #129721

    Purpose

    AAVS1 safe harbor targeting vector expressing miniIAA7-mScarlet-I tagged Seipin

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    Seipin (BSCL2 Human)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    miniIAA7-mScarlet-I

    Expression

    Mammalian

    Mutation

    silent mutations

    Promoter

    EF1a

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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