We narrowed to 26,138 results for: Vit

1. pT3TS nErCas12an

   Plasmid

   #132642

   Purpose

   Used for in vitro transcription of dual NLS ErCas12a mRNA

   Depositor

   Stephen Ekker

   Article

   Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

   Insert

   ErCas12a
   

   Use

   CRISPR; In vitro transcription

   Tags

   SV40 NLS

   Expression

   Mutation

   Promoter

   T3

   Available since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. HaloTag-3xrGBD

   Plasmid

   #176108

   Purpose

   Visualization of active Rho

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   rhotekin (Rtkn Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMVdel

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21a Ov-iPGM

   Plasmid

   #180245

   Purpose

   Bacterial expression plasmid for production of recombinant Onchocerca volvulus iPGM His10

   Depositor

   James Inglese

   Article

   Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

   Insert

   Onchocerca volvulus iPGM-10His
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21a Ce-iPGM-bio

   Plasmid

   #162559

   Purpose

   Expresses C. elegans iPGM containing a C-terminal biotinylation site and His tag

   Depositor

   James Inglese

   Article

   Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

   Insert

   C. elegans iPGM bio-6His (ipgm-1 Nematode)
   

   Use

   Tags

   6His tag, T7 tag, Thrombin cleavage site, and bio…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21a Bm-iPGM-bio

   Plasmid

   #162565

   Purpose

   Expresses B. malayi iPGM containing a C-terminal biotinylation site and His tag

   Depositor

   James Inglese

   Article

   Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

   Insert

   B. malayi iPGM bio-6His
   

   Use

   Tags

   6His tag, T7 tag, biotinylation sequence, and thr…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5.1-V5-HisB-ObKHC-wt-HaloTag

   Plasmid

   #201551

   Purpose

   Expresses wild-type Octopus kinesin-1 motor with HaloTag in Drosophila S2 cells

   Depositor

   Joshua Rosenthal

   Article

   Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

   Insert

   Kinesin-1 (partial)
   

   Use

   Tags

   HaloTag

   Expression

   Insect

   Mutation

   Codon-optimized for Drosophila expression

   Promoter

   Ac5

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-REPORT(EF1a)

   Plasmid

   #172326

   Purpose

   LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   d2eGFP
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP gRNA (BRDN0000561167)

   Plasmid

   #80034

   Purpose

   3rd generation lentiviral gRNA plasmid targeting EGFP

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF1α:flex-mcherry

   Plasmid

   #222618

   Purpose

   Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

   Depositor

   Franck Polleux

   Article

   Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

   Insert

   Cre-dependent mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMIA4.721-Keppel

    Plasmid

    #214444

    Purpose

    BxbI landing-pad HDR donor for targeting Keppel-19 GSH. CAGG-driven Casp9-P2A-Bsd.

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    2xFKBP12-Casp9
    

    Use

    Hdr donor

    Tags

    P2A-Bsd

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV3Tag8li-hHP1BP3-HA

    Plasmid

    #165468

    Purpose

    Human HP1BP3 mammalian expression vector with C-terminal HA tag.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    Heterochromatin protein 1 binding protein 3 (HP1BP3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-GIT1

    Plasmid

    #15226

    Depositor

    Rick Horwitz

    Article

    Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

    Insert

    G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L425V, E476K and L583P

    Promoter

    Available since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

    Plasmid

    #75154

    Purpose

    Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    H2B-mCerulean3-P2A-mKOFP2-CAAX
    

    Use

    Middle entry vector for multisite gateway three f…

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-CMV-Galpha-i2 ONE-GO

    Plasmid

    #189736

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-i2 ONE-GO
    GNAI2 (Gnai2 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. Myc-Bnip3FL

    Plasmid

    #100796

    Purpose

    Mammalian expression of Bnip3 full length splice variant

    Depositor

    Joseph Gordon

    Article

    Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

    Insert

    mus Bnip3 (Bnip3 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. VRQR variant

    Plasmid

    #138562

    Purpose

    Expresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRQR
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335Q, T1337R

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor

    Plasmid

    #12153

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Use

    Retroviral

    Tags

    6XHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBSKΔBS-SD_MCS_SA(NBSI)-24xMS2-sirius-96xopto-TetO-Int-3xFLAG-NeoR-GS4

    Plasmid

    #177265

    Purpose

    STREAMING-tag cassette

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag cassette
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJT121_GalL_nCDA1-VQRBE3

    Plasmid

    #145086

    Purpose

    Expressing base editor nCDA1-VQRBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    nCDA1-VQRBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A, D1135V, R1335Q, T1337R)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Syn-SomArchon

    Plasmid

    #126941

    Purpose

    Expression of SomArchon under Syn promoter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

    Insert

    SomArchon
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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