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Plasmid#41119DepositorTypeEmpty backboneUseTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only
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pNIC-NH10-GG
Plasmid#215806PurposeCloning of target gene with N terminal non cleavable His10 tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOPINS3C
Plasmid#41115DepositorTypeEmpty backboneUseTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOPINHALO7
Plasmid#41117DepositorTypeEmpty backboneUseTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOPINMSYB
Plasmid#41118DepositorTypeEmpty backboneUseTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOPINTF
Plasmid#41114DepositorTypeEmpty backboneUseTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNIC-CH10-GG
Plasmid#215807PurposeCloning of target gene with C terminal non cleavable His10 tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
INT_Cargo_PgyrA_gfp
Plasmid#212138PurposeCargo plasmid for integrating PgyrA expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.DepositorInsertgreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINTRX
Plasmid#41116DepositorTypeEmpty backboneUseTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
PHB1
Plasmid#212153PurposeFor PHB production in E. coli (PT7-pMB1).DepositorInsertpoly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
UseTagsExpressionBacterialMutationPromoterPT7Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB88
Plasmid#26958DepositorInserte. coli galactokinase
UseTagsExpressionBacterialMutationcloned under the MOP promoterPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBAT4
Plasmid#112580PurposeUsed for expression of protein in E. coli as non-fusionsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKDuet20
Plasmid#41694DepositorInsertMjaTFIIB intein
UseTagsGB1 and His-tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSKDuet23
Plasmid#41697DepositorInsertPhoPol-II intein
UseTagsGB1 and His-tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSKDuet22
Plasmid#41696DepositorInsertPhoCDC21-2 intein
UseTagsGB1 and His-tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSKDuet21
Plasmid#41695DepositorInsertPhoCDC21-1 intein
UseTagsGB1 and His-tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOliSpec5'
Plasmid#228551PurposeTemplate for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmidDepositorInsertTruncated 5' region of aadA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOliSpec3'
Plasmid#228550PurposeTemplate for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.DepositorInsertTruncated 3' region of aadA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCT03
Plasmid#212150PurposePlasmid expressing alanine dehydrogenase under inducible promoter PT7 control. The enzyme is thermophilic and works best at 50 C.DepositorInsertalanine dehydrogenase
UseTags6x HisExpressionBacterialMutationPromoterPT7Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKP2299
Plasmid#167627PurposeExpress TonB C18G, H20A, A209C.DepositorInserttonB C18G, H20A, A209C
UseTagsExpressionBacterialMutationtonB with C18G, H20A, A209C mutationsPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1861
Plasmid#167629PurposeExpress TonB C18G, H20A, R212C.DepositorInserttonB C18G, H20A, R212C
UseTagsExpressionBacterialMutationtonB with C18G, H20A, R212C mutationsPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1723
Plasmid#167631PurposeExpress TonB C18G, H20A, R214C.DepositorInserttonB C18G, H20A, R214C
UseTagsExpressionBacterialMutationtonB with C18G, H20A, R214C mutationsPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2303
Plasmid#167632PurposeExpress TonB C18G, Q160C, F202A.DepositorInserttonB C18G, Q160C, F202A
UseTagsExpressionBacterialMutationtonB with C18G, Q160C, F202A mutationsPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1692
Plasmid#167625PurposeExpress TonB C18G, H20A, N208C.DepositorInserttonB C18G, H20A, N208C
UseTagsExpressionBacterialMutationtonB with C18G, H20A, N208C mutationsPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1722
Plasmid#167623PurposeExpress TonB C18G, H20A, V206C.DepositorInserttonB C18G, H20A, V206C
UseTagsExpressionBacterialMutationtonB with C18G, H20A, V206C mutationsPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1868
Plasmid#167621PurposeExpress TonB C18G, H20A, R204C.DepositorInserttonB C18G, H20A, R204C
UseTagsExpressionBacterialMutationtonB with C18G, H20A, R204C mutationsPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1720
Plasmid#167619PurposeExpress TonB C18G, H20A, M201C.DepositorInserttonB C18G, H20A, M201C
UseTagsExpressionBacterialMutationtonB with C18G, H20A, M201C mutationsPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1708
Plasmid#167617PurposeExpress TonB C18G, H20A, Q160C.DepositorInserttonB C18G, H20A, Q160C
UseTagsExpressionBacterialMutationtonB with C18G, H20A, Q160C mutations.PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHL1948
Plasmid#53046PurposePconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHindM10:RBS(st3):csrADepositorInsertsGFP
tetR
csrA
UseSynthetic BiologyTagsRBS(st3) and glgC leaderExpressionBacterialMutationPromoterpConNoHind, pConNoHindM10, and pConNoHindM12Available sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1947
Plasmid#53045PurposePconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHindM8:RBS(st3):csrADepositorInsertsGFP
tetR
csrA
UseSynthetic BiologyTagsRBS(st3) and glgC leaderExpressionBacterialMutationPromoterpConNoHind, pConNoHindM12, and pConNoHindM8Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only