We narrowed to 5,258 results for: pcDNA3.1+

1. pcDNA3.1 LIC 6A HIS-TEV-DPP9S

   Plasmid

   #166791

   Purpose

   Mammalian cell expression vector for His-tagged DPP9S

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Use

   Tags

   HIS-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1 Flag-HIF1B [F446L] (#931)

   Plasmid

   #99917

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Chen et al Nature. 2016 Nov 3;539(7627):112-117. do

   Insert

   HIF1B (ARNT Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   F446L

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-DnaJB1-PKAcat(K72H)-mCherry

   Plasmid

   #181851

   Purpose

   mCherry-tagged DnaJB1-PKA catalytic subunit fusion; contains catalytically dead PKA; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   DnaJB1-PKAcat(K72H)-mCherry (PRKACA, DNAJB1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Lysine 128 in the DnaJB1-PKAcat fusion changed to…

   Promoter

   CMV

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-dCas9-MQ1(S317A)-EGFP

   Plasmid

   #89636

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR

   Tags

   3*FLAG and 6*His

   Expression

   Mammalian

   Mutation

   S317A, AGC-->GCC

   Promoter

   CMV

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1/Myc-His(-)-HSulf-1 deltaCC

   Plasmid

   #13005

   Depositor

   Steven Rosen

   Article

   Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

   Insert

   human sulfatase 1 delta CC (SULF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Cysteine 87 to Alanine

   Promoter

   Available since

   Oct. 30, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pCDNA3.1-Kozak-SP-SNAPf-lk-EK-ADGRL3-CTF

   Plasmid

   #201217

   Purpose

   pCDNA3.1 with Kozak (GCCACC), ADGRL3 signal peptide, SNAP-tag, GS linker, and Flag-tag (EK site) ahead of ADGRL3-CTF

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Use

   Tags

   Flag and SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1 FLAG-SIV-RCM-vpx

   Plasmid

   #115844

   Purpose

   Encodes codon optimized FLAG tagged SIVRCM-Vpx

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   SIVRCM-Vpx
   

   Use

   Tags

   3x-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-CD81-(TEV)-FKBP-Myc

   Plasmid

   #177908

   Purpose

   human CD81-TEV cleavage site-FKBP-Myc tag

   Depositor

   Masaharu Somiya

   Article

   Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

   Insert

   human CD81 (CD81 Human, Synthetic)
   

   Use

   Tags

   FKBP, Myc tag, and TEV protease cleavage site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1 SARS-CoV-2 S D614G-trunc

   Plasmid

   #158077

   Purpose

   Expresses SARS-CoV-2 spike protein with a D614G truncated mutation

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   Available since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 LIC 6A FLAG-TEV-DPP8

    Plasmid

    #166795

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP8

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP8 (DPP8 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NC3 pCDNA3.1 FLAG WTX (1-804)

    Plasmid

    #36958

    Depositor

    Randall Moon

    Article

    Major et al Science. 2007 May 18;316(5827):1043-6.

    Insert

    WTX (AMER1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286A)

    Plasmid

    #172647

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286A) in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Thr286Ala

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1 LIC 6A HIS-TEV-DPP8

    Plasmid

    #166793

    Purpose

    Mammalian cell expression vector for Flag-tagged DPP8

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP8 (DPP8 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST

    Plasmid

    #127312

    Purpose

    Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(+) Laccase2 MCS-ciRS7 Exon

    Plasmid

    #69900

    Purpose

    Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ciRS7 (stw, CDR1-AS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. (137) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4)

    Plasmid

    #160740

    Purpose

    Expresses a truncated OGT (4.5 TPRs) fused to the EPEA nanobody

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    HA-nEPEA-(EAAAK)4-OGT(4)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-dCas9-MQ1(C141S)-EGFP

    Plasmid

    #89635

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG, 6*His, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    C141S, TGC-->TCC

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-tvD-Cit-tevs-DHFR

    Plasmid

    #116054

    Purpose

    Reporter plasmid for measuring protease activity

    Depositor

    Michael Elowitz

    Article

    Gao et al Science. 2018 Sep 21;361(6408):1252-1258

    Insert

    tvD-Cit-tevs-DHFR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(+) ZKSCAN1 MCS-HIPK3 Exon

    Plasmid

    #69905

    Purpose

    Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    HIPK3 (HIPK3 Human, Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Expresses aa1-194 of HIPK3

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+)-Myr-DnaJB1-PKAcat-mCherry

    Plasmid

    #181853

    Purpose

    mCherry-tagged DnaJB1-PKA catalytic subunit fusion with N-terminal myristoylation site added; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1 LIC 6A CARD8-FLAG S297A

    Plasmid

    #169949

    Purpose

    Mammalian expression of CARD8-FLAG that is autoprocessing-deficient (S297A)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S297A

    Promoter

    CMV

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1(-) Full length Her2(ERBB2)-Fc

    Plasmid

    #194330

    Purpose

    Mammalian expression of full length Human epidermal growth factor (Her)-2-Fc fusion protein

    Depositor

    Alexander McLellan

    Insert

    Her2-Fc (ERBB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1/CIP2A(1-905) R522D V5 His

    Plasmid

    #119289

    Purpose

    CIP2A R522D V5 overexpression, CIP2A dimer defective mutant

    Depositor

    Jukka Westermarck

    Article

    Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

    Insert

    KIAA1524 (CIP2A Human)
    

    Use

    Tags

    V5 His

    Expression

    Mammalian

    Mutation

    R522D

    Promoter

    T7

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1 SARS-CoV-2 S D614-trunc

    Plasmid

    #158076

    Purpose

    Expresses SARS-CoV-2 spike protein with a truncation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1 FLIPglu-30uDelta13V ECFP-W67G

    Plasmid

    #18017

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    FLIPglu D154A Delta13V ECFP-W67G
    

    Use

    Tags

    ECFP-W67G and Venus

    Expression

    Mammalian

    Mutation

    mglB-D154A; non-functional ECFP

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1 GIL-11 myc TEV TwinStrep

    Plasmid

    #199627

    Purpose

    Eucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tag

    Depositor

    Jurgen Scheller

    Article

    Rafii et al Commun Biol. 2023 Apr 15;6(1):418. doi:

    Insert

    GIL-11
    

    Use

    Tags

    TwinStrep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1/Myc-His(-)-HSulf-2 deltaCC

    Plasmid

    #13006

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 2 deltaCC (SULF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Cysteine 88 to Alanine

    Promoter

    Available since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

28. pcDNA3.1-CMV-CIBN-mCherry-VAMP2

    Plasmid

    #178573

    Purpose

    Mammalian expression plasmid of CIBN-VAMP2 of Opto-vTrap

    Depositor

    Won Do Heo

    Article

    Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

    Insert

    CIBN-mCherry-VAMP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. XE257 pCDNA3.1(zeo)-hDsh3-truncDEP

    Plasmid

    #16752

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    FLAG-hDsh3-trunc before DEP (DVL3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    truncated before DEP

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EGFP Rab11a S25N pcDNA3.1 Zeo (+)

    Plasmid

    #206080

    Purpose

    dominant-negative Rab11a mutant - S25N mutation causes inhibition of Rab11a-dependent recycling

    Depositor

    Annette Dolphin

    Article

    Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

    Insert

    RAB11A (RAB11A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S25N

    Promoter

    CMV

    Available since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1 FLIPglu-30uDelta13V Venus-Y67G

    Plasmid

    #18016

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    FLIPglu D154A Delta13V Venus-Y67G
    

    Use

    Tags

    ECFP and Venus-Y67G

    Expression

    Mammalian

    Mutation

    mglB-D154A; non-functional Venus

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1 6B CARD8 UPA-CARD-mCherry WT

    Plasmid

    #164011

    Purpose

    Mammalian expression vector encoding CARD8 UPA-CARD with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1-mas-GRK2RH-Nluc L118A

    Plasmid

    #158606

    Purpose

    Plasmid expressing GRK2(RH)-Nluc L118A in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain L118A - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C261

    Plasmid

    #171429

    Purpose

    Expression of FKBP-(GGS)X6-HaloTagM C261 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    FKBP-(GGS)X6-HaloTag C261
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCDNA3.1(+)-HaloTag N58-(GGS)X6-hH2A

    Plasmid

    #171785

    Purpose

    Expression of HaloTag N58-(GGS)X6-hH2A in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    HaloTag N58-(GGS)X6-hH2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1V5-His Snk/Plk2 D223N

    Plasmid

    #22218

    Depositor

    Wafik El-Deiry

    Article

    Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

    Insert

    Snk/Plk2 (PLK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    single amino acid substitution mutant, aspartic a…

    Promoter

    Available since

    Aug. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

37. pcDNA3.1(+) Laccase2 MCS-HIPK3 Exon

    Plasmid

    #69899

    Purpose

    Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    HIPK3 (stw Human, Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Expresses aa1-194 of HIPK3

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. (365) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4)

    Plasmid

    #168188

    Purpose

    HA-tagged Ubc6E nanobody fused to a truncated OGT of 4 TPRs

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-nUbc6E-(EAAAK)4-OGT(4)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. NC4 pCDNA3.1 VENUS WTX (1-804)

    Plasmid

    #36959

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    WTX (AMER1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

40. pCDNA3.1(+)-hH2A-(GGS)X6-HaloTag C58

    Plasmid

    #171431

    Purpose

    Expression of hH2A-(GGS)X6-HaloTag C58 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    hH2A-(GGS)X6-HaloTag C58
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA3.1-KozATG-dH6.2-2XG4S-actin

    Plasmid

    #73263

    Purpose

    Expresses dH6.2-actin fusion within mammalian cells. (dH6.2, FAP)

    Depositor

    Marcel Bruchez

    Article

    Yan et al Chemphyschem. 2014 Mar 17;15(4):687-95.

    Insert

    KozATG-dH6.2-2XG4S-actin (ACTB Human, Synthetic)
    

    Use

    Tags

    The FAP and actin are fused with 2 copies of a G4…

    Expression

    Mammalian

    Mutation

    The dH6.2 FAP has one cysteine in each domain cha…

    Promoter

    CMV

    Available since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3.1-MS2-HA-EGFP-HsNanos2_6-22_X

    Plasmid

    #147929

    Purpose

    Mammalian Expression of HsNanos2_6-22

    Depositor

    Elisa Izaurralde

    Article

    Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

    Insert

    HsNanos2_6-22 (NANOS2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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43. pcDNA3.1-FLAG-Dicer-5'-pocket mutant

    Plasmid

    #51368

    Depositor

    Narry Kim

    Article

    Park et al Nature. 2011 Jul 13;475(7355):201-5. doi

    Insert

    Dicer (DICER1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    5' pocket mutant (R778A, R780A, R811A, H982A…

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

44. XE255 pCDNA3.1(zeo)-hDsh3-truncPDZ

    Plasmid

    #16754

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    FLAG-hDsh3-trunc before PDZ (DVL3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    truncated before PDZ

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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45. pcDNA3.1 myc-mβTrCP F-box (1-247)

    Plasmid

    #62976

    Purpose

    Expresses myc-tagged mouse βTrCP residues 1-247

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    BTRC (Btrc Mouse)
    

    Use

    Tags

    3x myc

    Expression

    Mammalian

    Mutation

    codes for residues 1-247

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

46. XE253 pCDNA3.1(zeo)-hDsh3-82-716

    Plasmid

    #16756

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    FLAG-82-716-Dsh3 (DVL3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    aa 82-716

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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47. pcDNA3.1-FLAG-SBP-STAG2 (S97X)

    Plasmid

    #73964

    Purpose

    STAG2 cDNA (CCDS43990) S97X

    Depositor

    Todd Waldman

    Article

    Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

    Insert

    STAG2 (STAG2 Human)
    

    Use

    Tags

    FLAG-SBP

    Expression

    Mammalian

    Mutation

    S97X

    Promoter

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

48. (81) pcDNA3.1-BC2-Nup62-Flag-EPEA

    Plasmid

    #168094

    Purpose

    Nucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tags

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    Nup62-Ubc-Flag-EPEA
    

    Use

    Tags

    BC2-Tag, EPEA-tag, and Flag-tag

    Expression

    Mammalian

    Mutation

    P229A, S283T on Nup62 native sequence

    Promoter

    T7/CMV

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. pcDNA3.1-MCS-BirA(R118G)-HA-MACROD1

    Plasmid

    #172581

    Purpose

    For transient expression of C-terminal BirA-tagged MACROD1

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD1 (MACROD1 Human)
    

    Use

    Tags

    BirA(R118G)-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG

    Plasmid

    #127308

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge