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pXD80ENSF10-Pct3-(+)-Eddh(cHis6)-Accs(ElA2)

Plasmid

#233869

Purpose

Expresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eddh (Elen_0501 Eggerthella lenta)

Tags

His

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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XZ363 EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export

Plasmid

#233382

Purpose

Optimized VEGF-sensing eLIDAR with VEGFR1 ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequence

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

VEGFR1(ecto+TM)-ADAR2dd

Tags

FLAG

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pMT2077

Plasmid

#228338

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF27-Pct3-(+)-Eldh2(nHis6)(GpDSM19378)

Plasmid

#233875

Purpose

Expresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh2 (GPA_25800 Gordonibacter pamelaeae DSM 19378)

Tags

His

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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80.a_pSTC0-zeo

Plasmid

#202338

Purpose

Design 80.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

80.a

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1-GSSGmut-dsRNAres_Q

Plasmid

#147338

Purpose

Insect Expression of DmDCP1-GSSGmut-dsRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmDCP1-GSSGmut-dsRNAres (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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HoxD del ds1

Plasmid

#131338

Purpose

gRNA to delete a nucleation site near HoxD. Use with HoxD del us1

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EVX2-gRNA-C-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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BII-ChPtW-eSpCas9

Plasmid

#133381

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

eSpCas9

Tags

HA

Expression

Mammalian

Mutation

eSpCas9 mutations

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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BII-C3H2B

Plasmid

#133387

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAWH-IRP

Plasmid

#133896

Purpose

Positive control when used with pMS22H-IRE. Expression of Gal4AD-IRP hybrid protein. Homology regions for recombination with pMS22H

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

IRP

Expression

Yeast

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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TAL3164

Plasmid

#41338

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-slc26a6-Left (LOC100005108 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-1188

Plasmid

#26338

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-1188 (Mir1188 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pFSW Syt1-C2B V304A,I367A IRES GFP

Plasmid

#133821

Purpose

Encodes the C2B domain of synaptotagmin 1 with membrane-penetrating residue mutations V304A,I367A for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

V304A, I367A

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2B D363,365N IRES GFP

Plasmid

#133822

Purpose

Encodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

D363,365N

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2A K189-192A IRES GFP

Plasmid

#133823

Purpose

Encodes the C2A domain of synaptotagmin 1 with poly-lysine patch mutations K189-192A for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

K189-192A

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2A D230,232N IRES GFP

Plasmid

#133824

Purpose

Encodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

D230,232N

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2A M173,F234A IRES GFP

Plasmid

#133825

Purpose

Encodes the C2A domain of synaptotagmin 1 with membrane-penetrating residue mutations M173,F234A for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

M173, F234A

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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evoBxb1

Plasmid

#222338

Purpose

Plasmid for expressing evolved Bxb1 in the mammalian cells

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved Bxb1

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-GNB1

Plasmid

#133856

Purpose

Mammalian expression of GNB1 N-terminally tagged with EGFP

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

GNB1 (GNB1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-p18-HA

Plasmid

#42338

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

p18 (LAMTOR1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only