Addgene

Showing: 741 - 760 of 7297 results

Results per page:

Sort results by:

pQLL-MAG w/o spacer

Plasmid

#218292

Purpose

Donor plasmid of MAG Transposon without spacer

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of MAG Transposon

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet-1:ChrA-C63S

Plasmid

#225080

Purpose

Expresses precursor peptide ChrA-C63S in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrA-C63S

Use

Tags

His6

Expression

Bacterial

Mutation

C63S

Promoter

T7

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYC-yhaM-RFP

Plasmid

#207651

Purpose

Overexpression of native L-cysteine desulfidase and RFP in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

yhaM

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
T5-pAc5.1 EGFP-EcoRV

Plasmid

#206481

Purpose

Schneider cell imaging

Depositor

Anne Ephrussi

Article

Salgania et al bioRxiv 2022.03.04.482790

Type

Empty backbone

Use

Tags

EGFP

Expression

Insect

Mutation

Promoter

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mFzd4CRDL(42-203)-6xHis_pAcGP67a

Plasmid

#216379

Purpose

Baculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cells

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Frizzled-4 (Fzd4 Mouse)

Use

Baculovirus

Tags

6xHis and GP64 signal sequence

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC150-KEIMA-VAPA

Plasmid

#212096

Purpose

Fluorescent reporter for VAPA clearance to acidic lysosomes

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

VAPA (VAPA Human)

Use

Piggybac

Tags

mKeima

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-XPACK-DNAJC5 (WT)

Plasmid

#205716

Purpose

Mammalian expression of XPACK-DNAJC5 (WT)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

XPACK (GCINSKRKD)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC150-FAM134C-GFP

Plasmid

#201932

Purpose

PiggyBac Vector expressing FAM134C-GFP

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

FAM134C (RETREG3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC150-TEX264ΔLIR-GFP

Plasmid

#201930

Purpose

PiggyBac Vector expressing TEX264ΔLIR-GFP

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

TEX264 (TEX264 Human)

Use

Tags

Expression

Mammalian

Mutation

F273A

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GpACR1-pcDNA3.1-mScarlet

Plasmid

#204964

Purpose

Expression of GpACR1 in fusion with mScarlet in mammalian cells

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

GpACR1

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV (+ enhancer)

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF mito-PAcGFP

Plasmid

#188905

Purpose

Expresses mitochondrial photoactivatable GFP

Depositor

Edwin Chapman

Article

Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

Insert

mitochondrial photoactivatable GFP

Use

Tags

Mitochondrial leader sequence: MSVLTPLLLRGLTGSARR…

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
T8-pAc5.1 lambdaN HA EcoRV

Plasmid

#206492

Purpose

Schneider cell imaging

Depositor

Anne Ephrussi

Article

Salgania et al bioRxiv 2022.03.04.482790

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcBAC3-POLY-pnRFP

Plasmid

#201680

Purpose

Boost the expression of pnRFP in mammalian cells (e.g., macrophage RAW 264.7 cells)

Depositor

Huiwang Ai

Article

Pang et al ACS Chem Biol. 2023 Jun 16;18(6):1388-13

Insert

813

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-Dm4EHP_AH

Plasmid

#148914

Purpose

Insect Expression of Dm4EHP

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

Dm4EHP (4EHP Fly)

Use

Tags

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-HA-DmGIGYF-dsRNAres_AH

Plasmid

#148928

Purpose

Insect Expression of DmGIGYF1-dsRNAres

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF1-dsRNAres (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-Dm4EHP_AH

Plasmid

#148930

Purpose

Insect Expression of Dm4EHP

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

Dm4EHP (4EHP Fly)

Use

Tags

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmHelz_AH

Plasmid

#148881

Purpose

Insect Expression of DmHelz

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

DmHelz (CG9425 Fly)

Use

Tags

Expression

Insect

Mutation

two silent an two non silent G1374S, R2076G mutat…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmHelz_AH

Plasmid

#148885

Purpose

Insect Expression of DmHelz

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

DmHelz (CG9425 Fly)

Use

Tags

Expression

Insect

Mutation

two silent an two non silent G1374S, R2076G mutat…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmRoquin_AD

Plasmid

#148525

Purpose

Insect Expression of DmRoquin

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin (roq Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmRoquin_AD

Plasmid

#148526

Purpose

Insect Expression of DmRoquin

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin (roq Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge