We narrowed to 9,773 results for: Pol
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Plasmid#208972PurposeRecruited ePhi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertePhi29(+exo)-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)
Plasmid#208970PurposeRecruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertPhi29(+exo)-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianPromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFPnls barcode-210-SV40 polyA
Plasmid#190876PurposeFor barcoded retrograde labelingDepositorInsertEGFPnls-barcode210
UseAAVMutationWTAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-no5UTR-Fluc-MtoL-V5-5BoxB-polyA-HhR_AC
Plasmid#148409PurposeInsect Expression of 5BoxB-polyA-HhRDepositorInsert5BoxB-polyA-HhR
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-CrPV-Rluc-6xbsMS2-PolyA-Malat_AC
Plasmid#148446PurposeMammalian Expression of 6xbsMS2-PolyA-MalatDepositorInsert6xbsMS2-PolyA-Malat
ExpressionMammalianAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HCV-RLuc-6xbsMS2-polyA-Malat_AC
Plasmid#148448PurposeMammalian Expression of 6xbsMS2-polyA-MalatDepositorInsert6xbsMS2-polyA-Malat
ExpressionMammalianAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-CMV-EGFP-PolB(K72A)-PAMmut-Hygro
Plasmid#176087PurposeEGFP fused to the N-terminus of POLB containing the mutation Lys72Ala, a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassetteDepositorInsertPolB (POLB Human)
UseLentiviralTagsEGFPExpressionMammalianMutationMutation in Lys72 to Ala, and mutation in the PAM…PromoterCMVAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-p2A-QF2-MCS-polyA
Plasmid#174026PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInsertp2A-QF2
UseZebrafish knock-in taggingAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(L301R/V303R)-IRES-neo
Plasmid#128664Purposemammalian expression of Flag-Polß(L301R/V303R) protein with neomycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationL301R/V303RPromoterCMVAvailable SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E10: attB4-polyhedrinp>3xFLAG-nostop-attB1r
Plasmid#162936PurposeGateway attB4/attB1r entry clone for baculovirus expression of 3xFLAG tagged proteins using the polyhedrin promoterDepositorInsertTriple FLAG epitope tag fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyAvailable SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r
Plasmid#162937PurposeGateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoterDepositorInsertHis6 fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyAvailable SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E06: attB4-polyhedrinp>MBP-nostop-attB1r
Plasmid#162935PurposeGateway attB4/attB1r entry clone for baculovirus expression of MBP tagged proteins using the polyhedrin promoterDepositorInsertMaltose-binding protein fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(L301R/V303R)-IRES-puro
Plasmid#128658Purposemammalian expression of Flag-Polß(L301R/V303R) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationL301R/V303RPromoterCMVAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E01: attB4-polyhedrinp>His6-nostop-attB1r
Plasmid#162932PurposeGateway attB4/attB1r entry clone for baculovirus expression of His6 tagged proteins using the polyhedrin promoterDepositorInsertHis6 fusion with AcMNPV polyhedrin promoter
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047)
Plasmid#149350PurposeMammalian expression vector for SpyCas9, puromycin resistance and human Polb guide RNADepositorInsertSpyCas9
UseCRISPRTagsFlag tagExpressionMammalianPromoterCbhAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(L301R/V306R)-IRES-puro
Plasmid#128656Purposemammalian expression of Flag-Polß(L301R/V306R) with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationL301R/V306RPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(K206A/K244A)-IRES-puro
Plasmid#128683Purposemammalian expression of Flag-Polß(K206A/K244A) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationK206A/K244APromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(TM/K206A/K244A)-IRES-puro
Plasmid#128684Purposemammalian expression of Flag-Polß(TM/K206A/K244A: L301R/V303R/V306R/K206A/K244A)) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlag tagExpressionMammalianMutationL301R/V303R/V303R/K206A/K244APromoterCMVAvailable SinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato
Plasmid#138610PurposeExpress TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backboneDepositorInsertsHyperactive AID
T7 RNA Polymerase
tdTomato
ExpressionMammalianAvailable SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9824: CMV-SV40_NLS-CasMINI-V3.1--3xFlag- c-Myc_NLS-polyA
Plasmid#176271PurposeExpression of CasMINI-V3.1 in mammalian cells.DepositorInsertCasMINI-V3.1
ExpressionMammalianPromoterCMVAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only