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Plasmid#108889PurposeExpression of Cavin3 fused to the N-terminus of mVenus for bimolecular fluorescence complementationDepositorInsertCavin3 (altered codon usage) (CAVIN3 Human, Synthetic)
UseTagsmVenus1-155ExpressionMammalianMutationaltered codon usagePromoterAvailable sinceMarch 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4
Plasmid#98975PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 4 and Cas9(D10A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK285 (Stag-3FLAG-Bsr)
Plasmid#72801PurposeC-terminal tagging with Stag-3FLAG using CRIPSR/Cas9DepositorInsertStag-3FLAG-Bsr
UseCRISPRTagsStag-3FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
SMC2 CRISPR
Plasmid#140649PurposeSMC2 tagging CRISPRDepositorInsertSMC2 (SMC2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-H2B(1-114)_wt
Plasmid#118252PurposeBacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNaDepositorInsertxlH2B(1-114)
UseTagsMxe-GyrA intein fused to chitin binding domainExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEAK-cKal7
Plasmid#145801Purposeexpresses Kalirin-7 with the c-front in mammalian cellsDepositorInsertcKalirin-7 (Kalrn Rat)
UseTagsnoneExpressionMammalianMutationPromoterEIF2aAvailable sinceJune 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1
Plasmid#98972PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 1 and Cas9(D10A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PIG-N3FLAG-TAF10
Plasmid#105990Purposeretrovirally express mouse TAF10 with 3*FLAG tag at N terminal, GFP marker and puro resistanceDepositorInsertFLAG tagged TAF10 (Taf10 Mouse)
UseRetroviralTags3*FLAGExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2
Plasmid#98973PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 2 and Cas9(D10A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
LB1366 (Bcl-2 promoter with MHS1234)
Plasmid#15388DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterial and MammalianMutationBcl-2 full promoter with IgH full enhancer MHS1234PromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
LB1376 (Bcl-2 P1 promoter with MHS1234)
Plasmid#15387DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterial and MammalianMutationBcl-2 P1 promoter with IgH full enhancer MHS1234PromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
LB1377 (Bcl-2 P2 promoter with MHS1234)
Plasmid#15386DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterial and MammalianMutationBcl-2 P2 promoter with IgH full enhancer MHS1234PromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1
Plasmid#98976PurposeCas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 1 and Cas9(N863A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MDH1-miR-222-PGK-GFP-Blast
Plasmid#31522DepositorInsertmiR-222 (Mir222 Mouse)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
LRG-2.1T-neo-sgROSA
Plasmid#105587Purposelentivirally express gRNA targeting ROSA26 locus with GFP marker and neo resistanceDepositorInsertgRNA targeting rosa26 locus
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
LB1361 (Bcl-2 promoter with MHS12)
Plasmid#15391DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsBcl-2-LuciferaseExpressionBacterial and MammalianMutationBcl-2 full promoter with MHS12PromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
TtGNN-TAF4-HFD
Plasmid#106381Purposedox inducible expression of GFP fused TAF4 HFD (827-909) with neo resistanceDepositorInsertGFP fused TAF4 HFD (827-909) (Taf4 Mouse)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterTREtightAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
MDH1-miR-196a-1-PGK-GFP-Blast
Plasmid#31509DepositorInsertmiR-196a1 (Mir196a-1 Mouse)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHL 1917
Plasmid#53024PurposeAmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1DepositorInsertsUseSynthetic BiologyTagsAsp terminator, RBS(st7), and T1 terminatorExpressionBacterialMutationPromoterpCon and pLlacO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-N3FLAG-MYB-TAD(251-327)
Plasmid#105611Purposetransient express MYB TAD fragment with 3*FLAG tag at N terminal in HEK 293T cellsDepositorInsertTAD fragment of MYB (mouse), amino acids 251-327 (Myb Mouse)
UseTags3*FLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only