We narrowed to 13,035 results for: SHI

1. cryS96

   Plasmid

   #67519

   Purpose

   αB-crystallin minipeptide fused to ELP (S96).

   Depositor

   Andrew MacKay

   Article

   Wang et al J Control Release. 2014 Oct 10;191:4-14.

   Insert

   mini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Synthetic, Human)
   

   Use

   Tags

   elastin-like polypeptide (ELP) S96

   Expression

   Bacterial

   Mutation

   mini-αB-crystallin contains residues 73–92 of aB-…

   Promoter

   T7

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

2. MSCV-BRD9-PGK-Puro-IRES-GFP

   Plasmid

   #75114

   Purpose

   Retroviral expression plasmid encoding wild type human BRD9

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-CAG-TagRFP658-P2A-GFP

   Plasmid

   #178971

   Purpose

   Expresses TagRFP658 and GFP in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

   Insert

   TagRFP658-P2A-GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-xCas9RA-P2A-Puro

   Plasmid

   #110871

   Purpose

   Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV_Actb HMEJ donor

   Plasmid

   #97318

   Purpose

   HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb HMEJ donor
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG109 H2B-FAST

   Plasmid

   #130722

   Purpose

   Expresses FAST (also called YFAST) fused to zebrafish histone H2B in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   H2B-FAST
   

   Use

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac1_GI.1_Q62C-A140C

   Plasmid

   #162581

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations Q62C-A140C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Glutamine 62 changed to Cysteine and Alanine 140 …

   Promoter

   polyhedring

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPR - EGFP sgRNA 3

   Plasmid

   #51762

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV_Actb NHEJ donor

   Plasmid

   #97316

   Purpose

   NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb NHEJ donor
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. WT1 KI gRNA1

    Plasmid

    #92312

    Purpose

    CRISPR-GFP-gRNA for cutting WT1

    Depositor

    Sean Palecek

    Article

    Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

    Insert

    WT1 (WT1 Human)
    

    Use

    Tags

    CRISPR GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. WT1 KI gRNA2

    Plasmid

    #92313

    Purpose

    CRISPR-GFP-gRNA for cutting WT1

    Depositor

    Sean Palecek

    Article

    Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

    Insert

    WT1 (WT1 Human)
    

    Use

    Tags

    CRISPR GFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV_Actb MMEJ donor

    Plasmid

    #97315

    Purpose

    MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb MMEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCV-BRD9_dBD-PGK-Puro-IRES-GFP

    Plasmid

    #75115

    Purpose

    Retroviral expression plasmid encoding a human BRD9 mutant lacking the bromodomain

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    deleted bromodomain

    Promoter

    Available since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

14. MSCV-Cterm_3xFL_BRD9-PGK-Puro-IRES-GFP

    Plasmid

    #75122

    Purpose

    Retroviral expression plasmid encoding wild type human BRD9 with a C terminal 3x FLAG-tag

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Tags

    3x FLAG-tag

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pET22-His-TEV-DOT1L-MM

    Plasmid

    #89611

    Purpose

    Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416

    Depositor

    Leemor Joshua-Tor

    Article

    Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

    Insert

    Dot1L VVEL293MM Mutant (DOT1L Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA 3.3 ss-V5-hFZD4 (aa 37-537)

    Plasmid

    #115787

    Purpose

    Expresses human FZD4 with N-terminal V5-tag in mammalian cells, contains artificial signal sequence upstream of tag.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    FZD4 (FZD4 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF1a_HH-EMX1 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

    Plasmid

    #176459

    Purpose

    Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMV

    Depositor

    Hui Yang

    Article

    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

    Insert

    HH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEN25-Ub Hs

    Plasmid

    #83232

    Purpose

    pHis-par2-Ub Hs

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    Ubiqutin (UBC Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-xBE3-P2A-Puro

    Plasmid

    #110870

    Purpose

    Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xCas9(3.7)-BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSCV-Myc-PGK-Puro-IRES-GFP

    Plasmid

    #75124

    Purpose

    Retroviral expression plasmid encoding wild type murine Myc

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    myelocytomatosis oncogene (Myc Mouse)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only