We narrowed to 1,922 results for: cas9 expression vector

1. pLV.5’eGFP/sgVIM

   Plasmid

   #170548

   Purpose

   Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP without homology arms

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Insert

   sgRNA targeting 5'- end of VIM
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCEC-red

   Plasmid

   #196040

   Purpose

   Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly

   Depositor

   Paola Branduardi

   Article

   Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Grna cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTAJAK-71 (pESC-NatMXsyn-USER)

   Plasmid

   #78232

   Purpose

   Empty vector, for the insertion of gRNA expression cassettes into the vector.

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAW016-2

   Plasmid

   #85613

   Purpose

   Cas9-tracrRNA integration vector for Bacillus subtilis

   Depositor

   Perry Chou

   Article

   Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

   Insert

   cas9-tracrRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV.5’-LMNA-eGFP-KI

   Plasmid

   #170545

   Purpose

   Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP flanked by homology arms for LMNA

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Inserts

   Left Homology arm for LMNA knockin
   Right Homology arm for VIM knockin
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV.5’VIM-eGFP-KI

   Plasmid

   #170547

   Purpose

   Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP flanked by homology arms for VIM

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Inserts

   Left Homology arm for VIM knockin
   Right Homology arm for VIM knockin
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDCC5

   Plasmid

   #59984

   Purpose

   Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

   Depositor

   Peter Duchek

   Article

   Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Insect

   Mutation

   D10A

   Promoter

   hsp70Bb

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWS158

   Plasmid

   #90517

   Purpose

   Cas9 gap repair vector - URA3

   Depositor

   Tom Ellis

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWS175

   Plasmid

   #90962

   Purpose

   Cas9 gap repair vector - HygR

   Depositor

   Tom Ellis

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pWS172

    Plasmid

    #90519

    Purpose

    Cas9 gap repair vector - HIS3

    Depositor

    Tom Ellis

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWS171

    Plasmid

    #90518

    Purpose

    Cas9 gap repair vector - LEU2

    Depositor

    Tom Ellis

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pWS174

    Plasmid

    #90961

    Purpose

    Cas9 gap repair vector - NatR

    Depositor

    Tom Ellis

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pWS176

    Plasmid

    #90963

    Purpose

    Cas9 gap repair vector - ZeoR

    Depositor

    Tom Ellis

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBBK19 pCAS-Tyr-[gRNA: 2=FF21] (SplitHygR, AmpR)

    Plasmid

    #179003

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)

    Plasmid

    #179002

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBBK24 pCAS-Tyr-[gRNA: 7=HIS3] (SplitHygR, AmpR)

    Plasmid

    #179008

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBBK12 pCAS-Tyr-[gRNA: 2=FF21] (SplitKanR, AmpR)

    Plasmid

    #178996

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR)

    Plasmid

    #178995

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBBK23 pCAS-Tyr-[gRNA: 6=ARS416] (SplitHygR, AmpR)

    Plasmid

    #179007

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBBK22 pCAS-Tyr-[gRNA: 5=ARS308] (SplitHygR, AmpR)

    Plasmid

    #179006

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge