We narrowed to 1,744 results for: prep

1. pCAGEN-Dre-nls (JDW 1131)

   Plasmid

   #229809

   Purpose

   A CAGGS-driven dre recombinase followed by a c-terminal 1x nls sequence

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   DRE recombinase-nls
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28A-Rab8A

   Plasmid

   #228880

   Purpose

   Expression of His-tagged Rab8A in E. coli

   Depositor

   Sebastian Mathea

   Article

   Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

   Insert

   RAB8A (RAB8A Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   residues 1-6 and 177-207 missing

   Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. VPS13B^Halo

   Plasmid

   #224584

   Purpose

   codon optimized Halo tagged VPS13B for expression in mammalian cells

   Depositor

   Pietro De Camilli

   Article

   Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

   Insert

   VPS13B^Halo (VPS13B Human)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME nls EGFP WPRE bGH pA (JDW 488)

   Plasmid

   #224540

   Purpose

   A Gateway compatible middle entry clone containing a 3x-NLS-EGFP for labeling the nucleus

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   nls-EGFP-WPRE-bGH-pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME nls BFP FLAG WPRE bGH pA (JDW 484)

   Plasmid

   #224539

   Purpose

   A Gateway compatible middle entry clone containing a 3x-NLS-mTagBFP2-FLAG for labeling the nucleus

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   nls-mTagBFP2-FLAG_WPRE-bGH-pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. TMEM16F-I521A-peGFP-N1

   Plasmid

   #228577

   Purpose

   Expresses mouse TMEM16F-I521A-eGFP in mammalian cells

   Depositor

   Huanghe Yang

   Article

   Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

   Insert

   TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   I521A plus a 3 amino acid N-terminal truncation (…

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. TMEM16F-I521E-peGFP-N1

   Plasmid

   #228578

   Purpose

   Expresses mouse TMEM16F-I521E-eGFP in mammalian cells

   Depositor

   Huanghe Yang

   Article

   Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

   Insert

   TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   I521E plus a 3 amino acid N-terminal truncation (…

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL4.26-Ccnd2PromoterRegion1(1.2kb)

   Plasmid

   #228058

   Purpose

   1.2 kb from mouse Ccnd2 promoter in front of firefly luciferase reporter

   Depositor

   Kenneth Chen

   Article

   Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

   Insert

   Ccnd2 promoter (Ccnd2 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.26-Ccnd2PromoterRegion2(0.5kb)

   Plasmid

   #228059

   Purpose

   0.5 kb from mouse Ccnd2 promoter in front of firefly luciferase reporter

   Depositor

   Kenneth Chen

   Article

   Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

   Insert

   Ccnd2 promoter (Ccnd2 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL4.26-Igf2Promoter

    Plasmid

    #228060

    Purpose

    0.9 kb from mouse Igf2 promoter in front of firefly luciferase reporter

    Depositor

    Kenneth Chen

    Article

    Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

    Insert

    Igf2 promoter (Igf2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST17-DXO

    Plasmid

    #177993

    Purpose

    For purification of N-terminally His-tagged DXO from bacteria

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    DXO (DXO Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. p3E-WPRE-bGH-pA (JDW 1221)

    Plasmid

    #224527

    Purpose

    A Gateway compatible 3' entry clone containing a WPRE upstream of Bovine growth hormone polyA to stabilize mRN

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    WPRE-bGH
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-rTetR(SE)-EED-2A-mTurquoise

    Plasmid

    #225692

    Purpose

    Lentiviral expression of rTetR(SE) fused to EED and mTurquoise-NLS

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    rTetR-EED (EED Synthetic, Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xFLAG and T2A-mTurquoise

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-rTetR(SE)-HP1a-2A-mTurquoise

    Plasmid

    #225691

    Purpose

    Lentiviral expression of rTetR(SE) fused to HP1a and mTurquoise-NLS

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    HP1a (CBX5 Synthetic, Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xFLAG and T2A-mTurquoise

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET3a.H2B-K120C

    Plasmid

    #224706

    Purpose

    Expresses Human H2B K120C mutant in bacterial cells for fluorescent labelling of histone octamers

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    H2B-K120C (H2B Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K120C

    Promoter

    T7

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)

    Plasmid

    #224490

    Purpose

    Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Golgi-mTagBFP-P2A-H2A-iRFP
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. p5E lexOP-c-fos min pro (JDW 1254)

    Plasmid

    #224477

    Purpose

    Gateway compatible 5' entry clone containing a Lex Operon upstream of a c-fos minimal promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LexOp
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. p5E-dr_Unc (JDW 1365)

    Plasmid

    #224482

    Purpose

    Gateway compatible 5' entry clone containing the Zebrafish unc-503 promoter with a minimal c-fos promoter and b-globin intron

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Unc-503 promoter
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME-H2B-V5-n2StayGoldC4 (JDW 1354)

    Plasmid

    #224486

    Purpose

    Gateway compatible middle entry clone contianing Histone H2B fusion to V5-tagged (n2)StayGold(C4) (Nuclear StayGold fluorescent reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    H2B-V5-n2StayGoldC4
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-myr-mTagBFP2-Flag (JDW 1183)

    Plasmid

    #224492

    Purpose

    Gateway compatible middle entry clone containing a Myristoylated mTagBP2 with a c-terminal Flag tag (cell membrane BFP marker)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    myr-mTagBFP2-Flag
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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