We narrowed to 1,369 results for: sag

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pUB_SM-FLuc0_EMCV-Ren

Plasmid

#210590

Purpose

Expresses SM(FLAG)-nonoptimal Firefly luciferase, and EMCVIRES-driven Renilla luciferase

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Inserts

Spaghetti monster (FLAG)-nonoptimal FLuc
Optimal Renilla luciferase

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

EMCV IRES and UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_SM-FLuc100_EMCV-Ren

Plasmid

#210591

Purpose

Expresses SM(FLAG)-optimal Firefly luciferase, and EMCVIRES-driven Renilla luciferase

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Inserts

Spaghetti monster (FLAG)-optimal FLuc
Optimal Renilla luciferase

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

EMCV IRES and UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_HD_160-223AA

Plasmid

#221489

Purpose

NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 1-159AA N-terminal and 224-371AA C-te…

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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LacI pm

Plasmid

#218097

Purpose

Phagemid that expresses LacI but no phage proteins, used as a control

Depositor

Kevin Esvelt

Article

Gretton et al bioRxiv 2024.03.20.585782

Type

Empty backbone

Expression

Bacterial

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPRa_CHO-Bip_gRNA1

Plasmid

#183694

Purpose

CRISPRa gRNA expression vector for Chinese Hamster Bip (Hspa5)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

gRNA targeting chinese hamster Bip (Hspa5)

Use

CRISPR

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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TRIN-E-shTAF12.364

Plasmid

#105572

Purpose

Dox-inducible mir30 MYB shRNA/dsRED expression with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-MYB-TAD(251-327)

Plasmid

#105619

Purpose

bacterially express GST tagged MYB TAD

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

MYB-TAD domain (Myb Mouse)

Tags

GST

Expression

Bacterial

Mutation

amino acids 251-327

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-N3FLAG-TAF12-HFD(50-130)

Plasmid

#105615

Purpose

transient express TAF12 HFD with 3*FLAG tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12-HFD amino acid 50-130 with FLAG tag (Taf12 Mouse)

Tags

3*FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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LENC-shTAF12.364

Plasmid

#105579

Purpose

retrovirally express TAF12 shRNA with Neo resistance and mCherry marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 shRNA #364

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-TADA1-HFD(140-230)

Plasmid

#105984

Purpose

transient express TADA1-HFD fragment in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TADA1-HFD (140-230) (Tada1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry_C1 I3

Plasmid

#61398

Purpose

Expresses mCherry-tagged Inhibitor-3 in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-GFP-I3

Plasmid

#61401

Purpose

Expresses GFP-tagged Inhibitor-3 in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pBacPak-FLAG-HIS-Sgf11

Plasmid

#52287

Purpose

Expresses Drosophila Sgf11

Depositor

Jerry Workman

Article

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

Sgf11 (Sgf11 Fly)

Tags

HIS, FLAG

Expression

Insect

Available Since

May 15, 2014

Availability

Academic Institutions and Nonprofits only

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pBacPak-HIS-FLAG-CG9866

Plasmid

#52290

Purpose

Expresses Drosophila Ataxin-7

Depositor

Jerry Workman

Article

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

Ataxin-7 (CG9866 Fly)

Tags

HIS FLAG

Expression

Insect

Available Since

April 24, 2014

Availability

Academic Institutions and Nonprofits only
LB1365 (Bcl-2 promoter with MHS34)

Plasmid

#15389

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 full promoter with MHS34

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
LB1362 (Bcl-2 promoter with MHS3)

Plasmid

#15390

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 full promoter with MHS3

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pLenti-DsRed_IRES_EGFP

Plasmid

#92194

Purpose

Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

DsRed-IRES-EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pMK290 (mAID-mClover-Hygro)

Plasmid

#72828

Purpose

C-terminal tagging with mAID-mClover using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mClover-Hygro

Use

CRISPR

Tags

mAID-mClover

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMK289 (mAID-mClover-NeoR)

Plasmid

#72827

Purpose

C-terminal tagging with mAID-mClover using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mClover-Neo

Use

CRISPR

Tags

mAID-mClover

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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AIO-Puro

Plasmid

#74630

Purpose

All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing

Depositor

Steve Jackson

Article

Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

Type

Empty backbone

Use

CRISPR

Tags

Puromycin resistant marker

Expression

Mammalian

Promoter

Cbh

Available Since

May 6, 2016

Availability

Academic Institutions and Nonprofits only

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