We narrowed to 17,805 results for: Met;

1. pST1374-N-NLS-flag-linker-Cas9-D10A

   Plasmid

   #51130

   Purpose

   Cas9 nickase optimized for nuclear import.

   Depositor

   Xingxu Huang

   Article

   Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

   Insert

   Cas9 nickase
   

   Use

   CRISPR

   Tags

   Flag and NLS

   Expression

   Mammalian

   Mutation

   D10A (nickase mutant)

   Promoter

   CMV

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pB-CAG-FLEX-F14F15-BFP-(mCherry)'

   Plasmid

   #201831

   Purpose

   piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

   Depositor

   Ian Wickersham

   Article

   Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Inserts

   CAG
   BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pmScarlet-i_alphaTubulin_C1

   Plasmid

   #85047

   Purpose

   In vivo visualization of microtubules (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   Alpha-Tubulin (TUBA1B Human)
   

   Use

   Tags

   mScarlet-i

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHW393 (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR)

   Plasmid

   #85583

   Purpose

   cGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding domain (DBD)

   Depositor

   Paul Sternberg

   Article

   Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Insert

   Prab-3-GAL4-SK(DBD)-VP64
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Prab-3-GAL4-SK(DBD)-VP64

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pmCherry-CIBN-CreC

   Plasmid

   #26889

   Purpose

   Expresses C-terminal fragment of Cre fused to CIBN for blue light-induced activation of DNA recombination when used with CRY2-CreN. Also expresses mCherry.

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CIBN-CreC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa 1-170 of CIB1, aa 106-343 of Cre

   Promoter

   CMV

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTag-RFP-C-h-Rab7L1-c-Myc

   Plasmid

   #79804

   Purpose

   Express TagRFP labeled human Rab7L1.

   Depositor

   James Johnson

   Article

   Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

   Insert

   Rab7L1 (RAB29 Human)
   

   Use

   Tags

   TagRFP and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. APLF-c001

   Plasmid

   #173077

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   APLF (APLF Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   K360-V448, codon optimized

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMXS-IRES-Blast coSHMT2

   Plasmid

   #106299

   Purpose

   Expresses SHMT2

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   serine hydroxymethyltransferase 2 (SHMT2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Codon optimized

   Promoter

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mTagRFP-T-Gamma-Tubulin-17

   Plasmid

   #58017

   Purpose

   Localization: Centrosomes, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Gamma-Tubulin (TUBG1 Human)
   

   Use

   Tags

   mTagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. Str-STIM1-NN_ST-SBP-EGFP

    Plasmid

    #65262

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge