We narrowed to 14,584 results for: pET

1. pET151-GFP-PP-RPN2 (918-947)

   Plasmid

   #73747

   Purpose

   Expression of GFP-PP-RPN2 (918-947) fusion in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN2 (RPN2 Human)
   

   Use

   Tags

   6x His and GFP

   Expression

   Bacterial

   Mutation

   Construct possesses only residues 918-947 of RPN2

   Promoter

   T7

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pET151-GFP-PP-RPN2 (797-953)

   Plasmid

   #74228

   Purpose

   [Edit] Expression of GFP-PP-RPN2 (797-953) fusion in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN2 (RPN2 Human)
   

   Use

   Tags

   6x His and GFP

   Expression

   Bacterial

   Mutation

   Construct possesses only residues 918-953 of RPN2

   Promoter

   T7

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pET151-Vps4(101-437) L151D

   Plasmid

   #87736

   Purpose

   Bacterial expression of a monomeric variant of the S. cerevisiae Vps4 AAA ATPase cassette

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Use

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100, mutated Leu 151 to Asp

   Promoter

   T7

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-Hook3 Q147A, M151A, I154A

   Plasmid

   #74616

   Purpose

   Expresses a triple mutant truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Q147A, M151A, I154A

   Promoter

   T7

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-Hook3 N68A, W69A, K77A

   Plasmid

   #74619

   Purpose

   Expresses a triple mutant truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET151-hUCH37 (ISF1)(M148D/F149D)

   Plasmid

   #71388

   Purpose

   Expression of hUCH37 (M148D/F149D) in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Insert

   hUCH37 (ISF1) M148D/F149D (UCHL5 Human)
   

   Use

   Tags

   HIS-TEV sequence

   Expression

   Bacterial

   Mutation

   changed methionine 148 to aspartate, phenylalanin…

   Promoter

   T7

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pET151-hUCH37 (ISF1)(M148A/F149A)

   Plasmid

   #61933

   Purpose

   Expression of hUCH37 (M148A/F149A) in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Insert

   hUCH37 (ISF1) (UCHL5 Human)
   

   Use

   Tags

   6x HIS

   Expression

   Bacterial

   Mutation

   Methionine 148 and Phenylalanine 149 were mutated…

   Promoter

   T7

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)

   Plasmid

   #61941

   Purpose

   Co-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Inserts

   hUCH37 (ISF1) (UCHL5 Human)
   hNFRKB (1-117) (NFRKB Human)
   

   Use

   Tags

   6x HIS

   Expression

   Bacterial

   Mutation

   Construct ends at residue 117, stop codon introdu…

   Promoter

   T7

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

9. 213-petDuet1-GBD-LUMP--cdc42

   Plasmid

   #65973

   Purpose

   Dual expression of GBD-LUMP and cdc42

   Depositor

   Gerard Marriott

   Article

   Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

   Inserts

   LUMP
   cell division cycle 42 (CDC42 Human)
   

   Use

   Tags

   GBD (GTPase binding domain from ACK1; residues 44…

   Expression

   Bacterial

   Mutation

   constitutively active Cdc42 (Q61L)

   Promoter

   T7

   Available since

   July 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET11+PCNA3-CYP119(D77R/T214V)

    Plasmid

    #66138

    Purpose

    Expresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    D77R, T214V

    Promoter

    T7 promoter

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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