We narrowed to 17,677 results for: por
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Plasmid#132534PurposeHuman Rbl-2, codon optimised for Sf9 expression.DepositorAvailable SinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only
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pGL3 T2P 800
Plasmid#131147PurposeTo investigate activity of a 800 bp region upstream of mouse Trib2.DepositorInsert-800 - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)
TagsluciferaseExpressionMammalianAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3 T2P 2.6
Plasmid#131151PurposeTo investigate activity of a 2.6 kb region upstream of mouse Trib2.DepositorInsert-2.6kb - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)
TagsluciferaseExpressionMammalianAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3 T2P 500
Plasmid#131150PurposeTo investigate activity of a 500 bp region upstream of mouse Trib2.DepositorInsert-500 - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)
TagsluciferaseExpressionMammalianAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
A3H HapII R175/6E-Cas9n-UGI
Plasmid#119142PurposeBase editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EEDepositorInsertAPOBEC3H Haplotype II RR175/6EE-Cas9 nickase-UGI
UseCRISPRMutationA3H Hap II RR175/6EEPromoterCMVAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
N229A Lo-MetQ
Plasmid#118268PurposeN229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structureDepositorInsertMetQ (N229A) (metQ E. coli)
Tags10x His Tag and Enterokinase Cleavage SiteExpressionBacterialMutationChanged Asparagine (Asn) 229 to Alanine (Ala)PromoterT7 PromoterAvailable SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-V111FL114FI766F-HA-TagBFP
Plasmid#120911PurposeExpresses BFP tagged Ptch1-B (V111FL114FI766F)DepositorInsertPtch1 (Ptch1 Mouse)
TagsHA and TagBFPExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291, Y…PromoterCMVAvailable SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-V111FL114FW115A-HA-GFP
Plasmid#120908PurposeExpresses Ptch1-B (V111FL114FW115A)DepositorInsertPtch1 (Ptch1 Mouse)
TagsGFP and HAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-I766FV111F-HA-GFP
Plasmid#120907PurposeExpresses Ptch1-B (I766FV111F)DepositorInsertPtch1 (Ptch1 Mouse)
TagsGFP and HAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-Y233C-HA
Plasmid#120904PurposeExpresses Ptch1-B (Y233C) for function assaysDepositorInsertPtch1 (Ptch1 Mouse)
TagsHAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291, Y…PromoterCMVAvailable SinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-L234C-HA
Plasmid#120905PurposeExpresses Ptch1-B (L234C)DepositorInsertPtch1 (Ptch1 Mouse)
TagsHAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291, L…PromoterCMVAvailable SinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-D27S/Y100F
Plasmid#110827PurposeBacterial expression of E.coli DHFR D27S/Y100F mutantDepositorInsertecDHFR
ExpressionBacterialMutationD27 mutated to S27, Y100 mutated to F100PromoterT7Available SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-TPI_PTC160-xrRNA-4H
Plasmid#108378PurposeFRT integration of TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertPTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
ExpressionMammalianPromoterCMVAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-TPI_WT-xrRNA-4H
Plasmid#108377PurposeFRT integration of wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
ExpressionMammalianPromoterCMVAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHW564 (Pmyo-2::cGAL-N(C1A)::let-858 3'UTR)
Plasmid#107739Purposemutated cGAL-N (C1A) driver under the control of C. elegans myo-2 promoterDepositorInsertNLS::cGAL(DBD)::gp41-1-N-intein(C1A)
ExpressionWormMutationchange the first cysteine of gp41-1 N-intein to a…PromoterC. elegans myo-2 promoterAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX458-sgRNA_Ago1_4
Plasmid#73536PurposeExpresses SpCas9, GFP, and sgRNA targeting Ago1DepositorInsertAGO1
UseCRISPRExpressionMammalianPromoterhU6Available SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPN018
Plasmid#91664PurposeExpress sgRNA targeting human SCN2ADepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPN282
Plasmid#91665PurposeExpress sgRNA targeting human SDCCAG8DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPN283
Plasmid#91666PurposeExpress sgRNA targeting human SDCCAG8DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPN272
Plasmid#91643PurposeExpress sgRNA targeting human MMP16DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only