We narrowed to 53,425 results for: plasmid

1. CD44I-c011

   Plasmid

   #175449

   Purpose

   Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N178. Used for crystallography.

   Depositor

   Opher Gileadi

   Insert

   CD44 (CD44 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HA-Clover-HIF-1alpha S31D

   Plasmid

   #163370

   Purpose

   Express HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser31Asp) plasmid

   Depositor

   Violaine Sée

   Insert

   Hypoxia Inducible Factor 1 alpha S31D (HIF1A Human)
   

   Use

   Tags

   HA- and Clover-tagged

   Expression

   Mammalian

   Mutation

   S31D

   Promoter

   CMV Promoter

   Available since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HA-Clover-HIF-1alpha S31G

   Plasmid

   #163369

   Purpose

   Express HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser31Gly) plasmid

   Depositor

   Violaine Sée

   Insert

   Hypoxia Inducible Factor 1 alpha S31G (HIF1A Human)
   

   Use

   Tags

   HA- and Clover-tagged

   Expression

   Mammalian

   Mutation

   S31G

   Promoter

   CMV Promoter

   Available since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HA-Clover-HIF-1alpha S786D

   Plasmid

   #163368

   Purpose

   Express HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser786Asp) plasmid

   Depositor

   Violaine Sée

   Insert

   Hypoxia Inducible Factor 1 alpha S786D (HIF1A Human)
   

   Use

   Tags

   HA- and Clover-tagged

   Expression

   Mammalian

   Mutation

   S786D

   Promoter

   CMV Promoter

   Available since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HA-Clover-HIF-1alpha S786A

   Plasmid

   #163367

   Purpose

   Express HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser786Ala) plasmid

   Depositor

   Violaine Sée

   Insert

   Hypoxia Inducible Factor 1 alpha S786A (HIF1A Human)
   

   Use

   Tags

   HA- and Clover-tagged

   Expression

   Mammalian

   Mutation

   S786A

   Promoter

   CMV Promoter

   Available since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGBW-m4164342

   Plasmid

   #153553

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   accatg Kozak-START linker

   Expression

   Mammalian

   Mutation

   Gordon et al recoded

   Promoter

   Available since

   June 16, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pGBW-m4164341

   Plasmid

   #153552

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   accatg Kozak-START linker

   Expression

   Mammalian

   Mutation

   Gordon et al recoded

   Promoter

   Available since

   June 16, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pGBW-m4137385

   Plasmid

   #149542

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Human, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wt;18-aa Ctrunc

   Promoter

   CMV

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pGBW-m4046565

   Plasmid

   #149097

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pJ211_WT_med

    Plasmid

    #128849

    Purpose

    Medium copy number plasmid for expression of E. coli flagellin from its natural promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJ211_rha_WT_hi

    Plasmid

    #128853

    Purpose

    High copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTHRB-donor

    Plasmid

    #112333

    Purpose

    CRISPR donor plasmid to tag human transcription factor THRB with GFP

    Depositor

    Kevin White

    Insert

    THRB homology arms flanking EGFP-IRES-Neo cassette (THRB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZNF445-donor

    Plasmid

    #112332

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF445 with GFP

    Depositor

    Kevin White

    Insert

    ZNF445 homology arms flanking EGFP-IRES-Neo cassette (ZNF445 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    rs78585428, hg38:3:44446218:C>T, hg38:3:444466…

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZNF785-donor

    Plasmid

    #112317

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF785 with GFP

    Depositor

    Kevin White

    Insert

    ZNF785 homology arms flanking EGFP-IRES-Neo cassette (ZNF785 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    hg38:16:30582292:A>T, hg38:16:30582302:A>T,…

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p240_LTJ_sgRNAFoxp3K276X

    Plasmid

    #82675

    Purpose

    sgRNA targeting murine Foxp3 mutant. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting Foxp3 K276X
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p204_LTJ_sgRNACD45.2_R2

    Plasmid

    #82673

    Purpose

    sgRNA targeting murine CD45.2 region 2. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting CD45.2 region 2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p163_LTJ_sgRNACD90.1

    Plasmid

    #82677

    Purpose

    sgRNA targeting murine CD90.1. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting mouse CD90.1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pH3F-PYK1

    Plasmid

    #85774

    Purpose

    N-ICE plasmid pH3F-PYK1

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-HphMX-loxP-PYK1p-3FLAG
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MIP 227 CoMiP 4in1 wo shRNA p53

    Plasmid

    #63727

    Purpose

    To express Oct4, Klf4, Sox2, and c-Myc. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).

    Depositor

    Joseph Wu, Mark Kay

    Article

    Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103

    Insert

    Mini-intronic-plasmid intron (POU5F1, KLF4, SOX2, MYC Human)
    

    Use

    Tags

    IRES-dtomato

    Expression

    Mammalian

    Mutation

    codon-optimised

    Promoter

    SFFV

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FKBP1AA-c001

    Plasmid

    #53671

    Purpose

    bacterial expression plasmid

    Depositor

    Nicola Burgess-Brown

    Insert

    FKBP1AA
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Promoter

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only