We narrowed to 11,806 results for: nsf

1. pDGE463

   Plasmid

   #153234

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-syn-SnFR-gamma8-minWPRE

   Plasmid

   #165498

   Purpose

   AAV for glutamate reporter fused to TARP gamma-8

   Depositor

   Andrew Plested

   Article

   Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755

   Insert

   iGluSnFR extracellular domain fused to TARP gamma-8 via NETO2 TM domain (Cacng8 Synthetic, Rat)
   

   Use

   AAV

   Promoter

   hSynapsin

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-Flex-mRuby3-2A

   Plasmid

   #203846

   Purpose

   Neuron specific expression of a Cre-dependent mRuby3

   Depositor

   Ivo Spiegel

   Article

   Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

   Insert

   mRuby3
   

   Use

   AAV

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. GalT-moxDendra2

   Plasmid

   #89789

   Purpose

   mammalian expression of GC localized moxDendra2

   Depositor

   Erik Snapp

   Article

   Kaberniuk et al Chem Commun (Camb). 2017 Feb 9;53(13):21

   Insert

   GalT signal anchor transmembrane domain (B4GALT1 Human)
   

   Tags

   moxDendra2

   Expression

   Mammalian

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Nd1

   Plasmid

   #191523

   Purpose

   Expression of mitochondrial v8(HS1) ZF-DdCBE pair in mammalian cells (LB510-Nd1+RB54-Nd1); AAV packaging vector

   Depositor

   David Liu

   Article

   Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

   Insert

   v8(HS1) ZF-DdCBE pair LB510-Nd1+RB54-Nd1 AGKS
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG-mFzd4-ΔC(40-513)-3C-sGFP1-10_pVL1393

   Plasmid

   #216380

   Purpose

   Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 ΔC (residues 40-513) fused to split GFP (strands 1-10)

   Depositor

   William Weis

   Article

   Bruguera et al bioRxiv 2024.02.03.578714

   Insert

   Frizzled-4 (Fzd4 Mouse)
   

   Use

   Baculovirus

   Tags

   3C-splitGFP1to10opt-ETVV and HA signal sequence-F…

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. rAAV-CAG::FLEX-rev:ChR2HA:2a:PSAML141F,Y115F:GlyR

   Plasmid

   #32483

   Purpose

   Cre-dependent expression of ChR2 and PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP marker

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

   Insert

   ChR2HA-2a-PSAML141F,Y115F:GlyR
   

   Use

   AAV and Cre/Lox; Adeno-associated virus, flex swi…

   Tags

   ChR2 2A

   Promoter

   CAG

   Available Since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-AAV-GFAP-iGABASnFR2n-WPRE

   Plasmid

   #218872

   Purpose

   AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2n
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   S99A F104H R168P

   Promoter

   GFAP

   Available Since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-fCR-GFP

   Plasmid

   #22911

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

   Insert

   fugu calretinin promoter driving GFP
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-EF1a-PGD2-1.1

    Plasmid

    #214764

    Purpose

    AAV packaging plasmid for GRAB PGD2-1.1 fluorescent sensor under EF1alpha promoter

    Depositor

    Yulong Li

    Article

    Sang et al Cell. 2023 Dec 7;186(25):5500-5516.e21.

    Insert

    GRAB-PGD2-1.1 sensor
    

    Use

    AAV

    Promoter

    EF-1-alpha promoter

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUpstream2Lox

    Plasmid

    #164573

    Purpose

    Integration of 2 LoxN sites in the genome of P. berghei

    Depositor

    Olivier Silvie

    Inserts

    Green fluorescent protein - human dihydrofolate reductase (GFP-2A-hDHFR) (DHFR Human, Synthetic)
    bifunctional yeast enzyme cytosine deaminase and uridyl phosphoribosyl transferase (yFCU) (FCY1, FUR1 Budding Yeast)
    

    Use

    Cre/Lox

    Promoter

    Bidirectional PbEF1alpha promoter (PBANKA_1133300…

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCellFree_G03 TGFB1

    Plasmid

    #67080

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TGFB1 (TGFB1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pTS99 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-3C-MCS

    Plasmid

    #199352

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-3C-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagBFP-P2A-ALFA-3C

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTS111 pHAGE2 CMVtetO2 MCS-3C-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199364

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)

    Plasmid

    #125225

    Purpose

    Expresses NanoLuc-β2AR-AsLOV2(V416L) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV Fos YFP MS2

    Plasmid

    #131775

    Purpose

    Fos upstream msfYFP 24xMS2 Fos downstream for CRISPR tagging

    Depositor

    Jared Toettcher

    Article

    Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

    Insert

    msfYFP - 24x MS2 loops
    

    Use

    AAV and CRISPR

    Promoter

    n/a

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. All-in-one split-ADAR2

    Plasmid

    #170136

    Purpose

    AAV vector carrying ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPRIME-CMV-Neo-FF3

    Plasmid

    #11665

    Purpose

    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of Neomycin and miR30-based shRNA targeting firefly luciferase

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    FF3
    

    Use

    Lentiviral

    Tags

    Neo

    Expression

    Mammalian

    Available Since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1a-DIO-mCherry-WPRE

    Plasmid

    #202620

    Purpose

    Double floxed red fluorescent protein mCherry in AAV production vector expressed under the mammalian promoter (EF1a)

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    mCherry
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV Syn ChR E90R-T159C 2A tDimer

    Plasmid

    #52495

    Purpose

    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

    Depositor

    Thomas Oertner

    Article

    Wietek et al Science. 2014 Mar 27.

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    E90R, T159C

    Promoter

    human synapsin-1 and ribosomal skip sequence

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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