We narrowed to 31,954 results for: Eng

1. pHybr_r2a>m2a(silent)-srt-his

   Plasmid

   #124807

   Purpose

   HDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.

   Depositor

   Ferenc Scheeren, Martijn Verdoes

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   Murine IgG2a (Fc silent)
   

   Use

   CRISPR; Hdr template

   Tags

   Histag (HHHHHH) and Sortag (LPETGG)

   Expression

   Bacterial and Mammalian

   Mutation

   L234A/L235A/N297A

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-Cry2-mCherry- YAP-C-IDR

   Plasmid

   #166447

   Purpose

   Expresses fusion of CRY2PHR with mCherry and YAP-C-IDR

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   Cry2, mCherry, YAP-C-IDR(266-504) (YAP1, CRY2 Human)
   

   Use

   Lentiviral

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-Cry2-mCherry-TEAD4-IDR

   Plasmid

   #166449

   Purpose

   Expresses fusion of CRY2PHR with mCherry and TEAD4-IDR

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   Cry2, mCherry, TEAD4-IDR(89-230) (TEAD4, CRY2 Human)
   

   Use

   Lentiviral

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHybr_r2a>mG1-srt-his

   Plasmid

   #124802

   Purpose

   HDR template to knock-in mouse IgG1 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG1 producing cell lines.

   Depositor

   Ferenc Scheeren, Martijn Verdoes

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   Murine IgG1
   

   Use

   CRISPR; Hdr template

   Tags

   Histag (HHHHHH) and Sortag (LPETGG)

   Expression

   Bacterial and Mammalian

   Promoter

   -

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX459-gR2A_Hinge

   Plasmid

   #124811

   Purpose

   Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.

   Depositor

   Ferenc Scheeren, Martijn Verdoes

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   Cas9 – gRNA_R2A_hinge
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Bacterial and Mammalian

   Mutation

   R166H in PuroR (please see depositors comment bel…

   Promoter

   pUC

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-ERBB2-L755_T759delLRENT

   Plasmid

   #116339

   Purpose

   Lentiviral expression of ERBB2 L755_T759delLRENT

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Lentiviral

   Mutation

   L755_T759delLRENT

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-KRAS-A146V

   Plasmid

   #116422

   Purpose

   Lentiviral expression of KRAS A146V

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   KRAS (KRAS Human)
   

   Use

   Lentiviral

   Mutation

   A146V

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-ERBB2-G776delinsVC

   Plasmid

   #116331

   Purpose

   Lentiviral expression of ERBB2 G776delinsVC

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Lentiviral

   Mutation

   G776delinsVC

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-ERBB2-R678Q

   Plasmid

   #116347

   Purpose

   Lentiviral expression of ERBB2 R678Q

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Lentiviral

   Mutation

   R678Q

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-AMBRA1-501*-3xFLAG

    Plasmid

    #174160

    Purpose

    PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 501

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA1 (AMBRA1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Missing aa 501-1208

    Promoter

    EF1A

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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