We narrowed to 20,516 results for: omp

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pAAV.pU1a-SpCas9

Plasmid

#121507

Purpose

Expresses codon-optimized SpCas9 in mammalian cells.

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

SpCas9

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

U1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-16

Plasmid

#172746

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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FKBP-CD28TMD-NTEVp(H75S)

Plasmid

#137828

Purpose

MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-CD28TMD-NTEVp(H75S)

Tags

3x FLAG

Expression

Mammalian

Mutation

Mutated Histidine 75 to Serine in NTEVp fragment

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCAT009

Plasmid

#171635

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-B2M
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pSL3402 (pTnsABf_Pse)

Plasmid

#200901

Purpose

Expresses human codon-optimized Pseudoalteromonas TnsA-TnsB fusion from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

PseTnsA-TnsB

Tags

Internal NLS between TnsA-TnsB fusion

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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RC(WW)-LgBit

Plasmid

#208931

Purpose

Recognition domain for in vitro MAPK-activity sensor

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

WW domain of PIN1 (PIN1 Human)

Tags

HISx6, LgBit, and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xHA-Reptin

Plasmid

#51636

Purpose

Mamamlian expression of 3xHA tagged reptin, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

reptin (RUVBL2 Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-C.NR

Plasmid

#185672

Purpose

Expresses nuclease active eNme2-C.NR in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

eNme2-C.NR nuclease

Use

CRISPR

Expression

Mammalian

Mutation

eNme2-C S6P/G33E/A520E/S646R/V696F/R711G/V758I/Y7…

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPA1A

Plasmid

#19483

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA1A (HSPA1A Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA-DEST QKI

Plasmid

#19891

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

quaking homolog, KH domain RNA binding (QKI Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-G12-CheRiff-T2A-mCherry

Plasmid

#170275

Purpose

CheRiff-T2A-mCherry construct expression in mammlian cells. pAAV vector. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

CheRiff-T2A-mCherry

Use

AAV

Promoter

11xUAS with TATA-box minimal promoter

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-LifeAct-GFP

Plasmid

#190199

Purpose

Expresses the small actin binding peptide, LifeAct, with a GFP reporter

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

LifeAct-eGFP

Use

AAV

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSL1749 (pDonor, R*MmeI)

Plasmid

#160733

Purpose

Mini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST donor DNA

Expression

Bacterial

Promoter

N/A

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-EsCas13d

Plasmid

#108303

Purpose

Expresses E. coli codon optimized EsCas13d in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

EsCas13d

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Promoter

lac

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Leo1

Plasmid

#11061

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

Leo1 (LEO1 Human)

Expression

Mammalian

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pSLC-254

Plasmid

#124291

Purpose

Expression of vsfGFP-0. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a dimeric fluorophore.

Depositor

Swaine Chen

Article

Eshaghi et al Angew Chem Int Ed Engl. 2015 Nov 16;54(4

Insert

vsfGFP-0

Expression

Bacterial

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPH1

Plasmid

#19480

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPH1 (HSPH1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pSFFV-dNme2Cas9

Plasmid

#193190

Purpose

Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

dNme2Cas9

Tags

NLS and NLS-3xHA-NLS

Expression

Mammalian

Mutation

H588A, D16A

Promoter

SFFV

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pETM6-RgTALsyn

Plasmid

#100947

Purpose

Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

T7

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pIZ-Flag6His-Siwi

Plasmid

#50560

Purpose

Expresses FLAG, His tagged Siwi in silkworm cell line BmN4 or insect cells

Depositor

Yukihide Tomari

Article

Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

Insert

Siwi (Aubergine )

Tags

6xHIS and FLAG

Expression

Insect

Promoter

OpIE2

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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