We narrowed to 9,182 results for: Pol

1. GFP_CD47_LU

   Plasmid

   #65474

   Purpose

   GFP tagged CD47 with its own long 3'UTR

   Depositor

   Christine Mayr

   Article

   Berkovits et al Nature. 2015 Apr 20. doi: 10.1038/nature

   Inserts

   Signal peptide of CD47
   GFP
   CD47 coding region continued and long 3’UTR (with proximal polyadenylation signal changed from AAUAAA to ACUCAA).
   

   Expression

   Mammalian

   Available Since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-At-TRL

   Plasmid

   #32242

   Depositor

   Jay Hesselberth, Stan Fields

   Article

   Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

   Insert

   A. thaliana tRNA ligase (RNL Mustard Weed)
   

   Tags

   6xHistidine

   Expression

   Bacterial

   Promoter

   T7 RNA Polmyerase

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBACgus_Sumostar/LRRK2 1867-2186

   Plasmid

   #40386

   Depositor

   Michael J Fox Foundation MJFF

   Inserts

   SUMO
   LRRK2 (LRRK2 Human)
   

   Use

   Baculovirus

   Tags

   6xHis and HisTEV

   Mutation

   WT aa1867-2186

   Promoter

   polH

   Available Since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pU6_N7-sgRNA2

   Plasmid

   #190664

   Purpose

   Expresses U6-driven N7 sgRNA2 (poly-T mutated out), BsmbI dropout cassette for cloning

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   N7 sgRNA2
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBabe-hygro PyMT

   Plasmid

   #22305

   Depositor

   Filippo Giancotti

   Article

   Pylayeva et al J Clin Invest. 2009 Feb . 119(2):252-66.

   Insert

   Polyoma Middle T
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Oct. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pLV CMV-TERRA

   Plasmid

   #111375

   Purpose

   TERRA expression contains approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

   Depositor

   Klaus Holzmann

   Article

   Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

   Insert

   TERRA polyA
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRL277

   Plasmid

   #70255

   Purpose

   sacB plasmid with polylinker; streptomycin/spectinomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.

   Depositor

   Peter Wolk

   Article

   Cai et al J Bacteriol. 1990 Jun;172(6):3138-45.

   Type

   Empty backbone

   Use

   Conditionally lethal

   Available Since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNA

   Plasmid

   #99698

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAV

   Depositor

   George Church

   Type

   Empty backbone

   Use

   AAV

   Available Since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. PRExpress

   Plasmid

   #122486

   Purpose

   A non-leaky heat-inducible gene expression system for Drosophila. (GenBank: MF058598.1)

   Depositor

   Renato Paro

   Article

   Akmammedov et al Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.

   Inserts

   LacZ
   poly-PRE
   

   Expression

   Insect

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICH41421

    Plasmid

    #50339

    Depositor

    Nicola Patron, Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPICZA_CeTRIC-B1_Del

    Plasmid

    #87344

    Purpose

    Inducible expression of CeTRIC-B1 in P.Pichia strains

    Depositor

    Zhenfeng Liu

    Article

    Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

    Insert

    TRIC-B1
    

    Tags

    polyhistidine

    Expression

    Yeast

    Mutation

    Δ248-297

    Promoter

    AOX1

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICSL11015

    Plasmid

    #117499

    Purpose

    FAST-Red selectable marker Golden Gate Level 1 Position 1

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:TGCC:pOLE1:OLE1-RFP:OLE1t:GCAA:BpiI
    

    Tags

    RFP

    Expression

    Plant

    Promoter

    AtOLE1

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRL542

    Plasmid

    #70282

    Purpose

    ColK-based vector; provides a nicking function to permit conjugal transfer and bears a polylinker for insertion of methylase-encoding genes

    Depositor

    Peter Wolk

    Article

    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-mIFT88-EGFP

    Plasmid

    #149697

    Purpose

    Expresses mouse IFT88-EGFP in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Insert

    IFT88 (Ift88 Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    EF1 alpha

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRL271

    Plasmid

    #70254

    Purpose

    sacB plasmid with polylinker; chloramphenicol/erythromycin resistance: use as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.

    Depositor

    Peter Wolk

    Article

    Cai et al J Bacteriol. 1990 Jun;172(6):3138-45.

    Type

    Empty backbone

    Use

    Conditionally lethal

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLfa-PUb-EGFP-3'UTR#13

    Plasmid

    #176338

    Purpose

    The synthetic 3'UTR#13 for EGFP expression under the control of the Ae. aegypti polyubiquitin (PUb) promoter

    Depositor

    Zach Adelman

    Article

    Chae et al RNA Biol. 2021 Aug 31:1-9. doi: 10.1080/

    Insert

    3'UTR#13
    

    Expression

    Insect

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOET5 GII.4 Sydney noro-VLP

    Plasmid

    #218092

    Purpose

    Forms Sydney GII.4 Sydney norovirus-like particle in insect cells by expression of VP1 from strain GII.4/Sydney/NSW0514/2012/AU (GenBank ID: AFV08795).

    Depositor

    Minna Hankaniemi

    Insert

    noro-VLP
    

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. 5TEY (METTL3)

    Plasmid

    #101892

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    METTL3 (METTL3 Human)
    

    Use

    Baculovirus expression

    Tags

    6xHis, TEV

    Promoter

    polyhedrin

    Available Since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pMAH-pnGFP-Ultra

    Plasmid

    #157924

    Purpose

    Peroxynitrite sensor pnGFP-Ultra in mammalian cells (use with pMAH-POLY-eRF1(E55D))

    Depositor

    Huiwang Ai

    Article

    Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

    Insert

    pnGFP-Ultra
    

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pL3-TRE-LucGFP-2L

    Plasmid

    #11685

    Depositor

    Geoff Wahl

    Article

    Wong et al Nucleic Acids Res. 2005 . 33(17):e147.

    Insert

    Luciferase-EGFP
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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