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pQUAST>stop>shibire (ts1)

Plasmid

#24363

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

Shibire (ts1) (shi Fly)

Expression

Insect

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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pFasBac+GFP-CAMSAP2 CC+CKK

Plasmid

#59039

Purpose

for baculovirus expression of CAMSAP2 CC+CKK in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP2 CC+CKK domains (CAMSAP2 Human)

Tags

His and eGFP

Expression

Insect

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pUASP dsRed Rab11 DN

Plasmid

#44831

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab11 (Rab11 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Mutation

S25N (dominant negative)

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pUASP dsRed Rab7 CA

Plasmid

#44825

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab7 (Rab7 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Mutation

Q67L (constitutive active)

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pAc-HT-Dmp53

Plasmid

#85698

Purpose

expression of Drosophila p53 in Drosophila cells

Depositor

Licio Collavin, Giannino Del Sal

Article

Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

Insert

Dmp53 (p53 Fly)

Tags

His

Expression

Insect

Promoter

Ac5

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
pENTR-GFP-Patronin CC+CKK

Plasmid

#59048

Purpose

for baculovirus expression of Patronin CC+CKK (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

eGFP

Expression

Bacterial and Insect

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pENTR-GFP-Patronin CC 534–1457

Plasmid

#59050

Purpose

for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

eGFP

Expression

Bacterial and Insect

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET21-pelB-LaG-2

Plasmid

#172739

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-2

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Aalb_726

Plasmid

#176661

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pUb LwaCas13a + LwaCas13a Guide RNA

Plasmid

#176307

Purpose

Expresses LwaCas13a and its associated guide RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

Insert

LwaCas13a

Tags

HA tag

Expression

Insect

Mutation

E656G, I1060V, D1076G- please see depositor comme…

Promoter

Ubi-p63e

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Aaeg_763

Plasmid

#176658

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pUASP YFP Rab5 DN

Plasmid

#37694

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab5 (Rab5 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

S43N (dominant negative)

Promoter

GAL4

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
UAS-Xbp1-GFP

Plasmid

#80627

Purpose

expression of Xbp1-GFP (RA isoform) in Drosophila

Depositor

Pedro Domingos, Herman Steller

Article

Ryoo et al EMBO J. 2007 Jan 10;26(1):242-52. Epub 2

Insert

Xbp1 (Xbp1 Fly)

Tags

EGFP

Expression

Insect

Mutation

RA isoform

Promoter

UAS

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab5 CA

Plasmid

#37702

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab5 (Rab5 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q88L (constitutive active)

Promoter

GAL4

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pAGN33

Plasmid

#131105

Purpose

An integrative plasmid able to introduce a second copy of the pip gene in the chromosome of our conditional mutants

Depositor

Riccardo Manganelli

Article

Boldrin et al Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1

Insert

P-furA102tetO-pip

Expression

Bacterial

Mutation

the furA102 promoter was mutated with the inserti…

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop

Plasmid

#169370

Purpose

Expression of gRNA in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop

Use

CRISPR

Expression

Insect

Promoter

CPIJ039596

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pUASP YFP Rab11 WT

Plasmid

#37711

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab11 (Rab11 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pCI-Flag-NEDD4L∆WW2

Plasmid

#166708

Purpose

expresses NEDD4L with inactivating mutation in second WW domain in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L W453A

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 453 to alanine

Promoter

CMV promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pUASP dsRed Rab7 DN

Plasmid

#44826

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab7 (Rab7 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Mutation

T22N (dominant negative)

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pAGW-dpse-GAL4-DBD

Plasmid

#125153

Purpose

Gateway-compatible vector to express a fusion protein containing the GAL4-DBD (N-terminally) followed by the protein of interest under the control of a D.pseudoobscura enhancer and CP

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

D. pseudoobscura peak #102 enhancer and D. pseudoobscura CG13116 CP

Use

Gateway fly expression vector

Expression

Insect

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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