We narrowed to 15,953 results for: grna

1. pWS3868

   Plasmid

   #185745

   Purpose

   Sub-array backbone 4/4

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-T3-hCASeGFP-pA

   Plasmid

   #59766

   Purpose

   Expresses CAS9-eGFP fusion protein in mammalian cells.

   Depositor

   Wataru Fujii

   Article

   Fujii et al Nucleic Acids Res. 2013 Aug 30.

   Insert

   CAS9
   

   Tags

   Flag and eGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pH1v1

   Plasmid

   #60244

   Purpose

   gRNA expression by the H1 promoter

   Depositor

   Donald Zack

   Article

   Ranganathan et al Nat Commun. 2014 Aug 8;5:4516. doi: 10.1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   H1 promoter

   Available Since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAL-pgi_NT

   Plasmid

   #74067

   Purpose

   pAL374 plasmid carrying the pgi (NT) sgRNA, targeting the non-template strand of pgi, SpecR

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Insert

   sgRNA pgi (NT)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   ptac

   Available Since

   April 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAL-pyk_NT

   Plasmid

   #74072

   Purpose

   pAL374 plasmid carrying the pyk (NT) sgRNA targeting the non-template strand of pyk, SpecR

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Insert

   sgRNA pyk (NT)
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   ptac

   Available Since

   April 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJEC625

   Plasmid

   #174368

   Purpose

   Golden gate vector for gRNA cloning. Contains BbsI sites for insertion of the gRNA spacer as annealed oligos.

   Depositor

   James Chappell

   Article

   Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJF131B

   Plasmid

   #113326

   Purpose

   pTet-W108 scRNA.b2, pTet-RR2-sgRNA

   Depositor

   Jesse Zalatan

   Article

   Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

   Insert

   W108 scRNA_MS2 and sgRNA_RR2
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAL-pgi_T

   Plasmid

   #74066

   Purpose

   pAL374 plasmid carrying the pgi (T) sgRNA, targeting the template strand of pgi, SpecR

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Insert

   sgRNA pgi (T)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   ptac

   Available Since

   April 12, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pECNUS_P2

   Plasmid

   #194344

   Purpose

   sgRNA position entry clone 2

   Depositor

   Eunyoung Chae

   Article

   Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

   Insert

   sgRNA expression cassettes and LacZ
   

   Expression

   Plant

   Promoter

   Ath U6 promoter

   Available Since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pECNUS_P1

    Plasmid

    #194343

    Purpose

    sgRNA position entry clone 1

    Depositor

    Eunyoung Chae

    Article

    Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

    Insert

    sgRNA expression cassettes and LacZ
    

    Expression

    Plant

    Promoter

    Ath U6 promoter

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pECNUS_P3

    Plasmid

    #194345

    Purpose

    sgRNA position entry clone 3

    Depositor

    Eunyoung Chae

    Article

    Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

    Insert

    sgRNA expression cassettes and LacZ
    

    Expression

    Plant

    Promoter

    Ath U6 promoter

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pECNUS_P4

    Plasmid

    #194346

    Purpose

    sgRNA position entry clone 4

    Depositor

    Eunyoung Chae

    Article

    Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

    Insert

    sgRNA expression cassettes and LacZ
    

    Expression

    Plant

    Promoter

    Ath U6 promoter

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pECNUS_P5

    Plasmid

    #194347

    Purpose

    sgRNA position entry clone 5

    Depositor

    Eunyoung Chae

    Article

    Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

    Insert

    sgRNA expression cassettes and LacZ
    

    Expression

    Plant

    Promoter

    Ath U6 promoter

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pECNUS_P6

    Plasmid

    #194348

    Purpose

    sgRNA position entry clone 6

    Depositor

    Eunyoung Chae

    Article

    Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

    Insert

    sgRNA expression cassettes and LacZ
    

    Expression

    Plant

    Promoter

    Ath U6 promoter

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pECNUS_P7

    Plasmid

    #194349

    Purpose

    sgRNA position entry clone 7

    Depositor

    Eunyoung Chae

    Article

    Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

    Insert

    sgRNA expression cassettes and LacZ
    

    Expression

    Plant

    Promoter

    Ath U6 promoter

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. sgPal1 (p2Tol-U6-sgPal1-HygR)

    Plasmid

    #71483

    Purpose

    Expresses palindromic sgRNA for use in self-cloning CRISPR

    Depositor

    Richard Sherwood

    Article

    Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

    Insert

    sgPal1
    

    Promoter

    U6

    Available Since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAL-pyk_T

    Plasmid

    #74070

    Purpose

    pAL374 plasmid carrying the pyk (T) sgRNA targeting the template strand of pyk, SpecR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    sgRNA pyk (T)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    ptac

    Available Since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

18. Guide-Generating Vector

    Plasmid

    #125752

    Purpose

    Guide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assembly

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

    Insert

    Cys4-GFPdropout-SpCas9scaffold
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWT060e

    Plasmid

    #107905

    Purpose

    W2m.0-3, W2m.3-2 plasmid. Contains U6-sgRNA B (CCR5) and is part of the CAMERA2m system for cellular recording in mammalian cells

    Depositor

    David Liu

    Article

    Tang et al Science. 2018 Feb 15. pii: science.aap89

    Insert

    U6-sgRNA B (CCR5)
    

    Expression

    Mammalian

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB_p300_core-dCas9-EGFP_hygro

    Plasmid

    #226431

    Purpose

    PiggyBac transposon vector containing a dox inducible p300 core-dCas9 fusion protein and EGFP reporter. Hygromycin selection marker

    Depositor

    Alon Goren

    Inserts

    p300 core-dCas9-EGFP fusion protein
    rtTA3-IRES-Hygro
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    HA tag, P2A-EGFP, and p300 core

    Expression

    Mammalian

    Promoter

    Dox inducible minimal CMV and UbC Promoter

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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