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ZP45 Prickle-1

Plasmid

#16897

Depositor

Randall Moon

Publication

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

Prickle-1 (prickle1a Zebrafish)

Expression

Bacterial

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
ZE33 Prickle-1-GFPnopren-pCS2+

Plasmid

#17074

Depositor

Randall Moon

Publication

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Prickle-1 (prickle1a Zebrafish)

Use

Zebrafish expression

Tags

GFP

Mutation

Pk1GFPnopren deletes the C-terminal six amino aci…

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pDrVSP(C302S)-IRES2-EGFP

Plasmid

#80331

Purpose

Expresses Danio rerio VSP (without phosphatase activity) with separated EGFP in mammalian cell

Depositor

Yasushi Okamura

Publication

Hossain et al J Biol Chem. 2008 Jun 27;283(26):18248-5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Daino rerio voltage-sensing phosphatase (tpte Zebrafish)

Expression

Mammalian

Mutation

C302S

Promoter

CMV

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pT22iU66CriT(#387)

Plasmid

#184061

Purpose

cyclofen-inducible CRE activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Publication

Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

Insert

CRE-ERT2

Use

Zebrafish transgenesis (blue eyes)

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; gcry1:BFP -2

Plasmid

#173891

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Publication

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; gcry1: BFP

Use

Synthetic Biology

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0; cmlc2:EGFP

Plasmid

#230075

Purpose

Partial ablation using NTR2 and subsequent lineage tracing of regenerating hepatocytes in zebrafish.

Depositor

Sumeet Pal Singh

Publication

Hovhannisyan et al NPJ Regen Med. 2026 Mar 30. doi: 10.1038

Insert

lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0

Use

Cre/Lox; Zebrafish expression

Promoter

fabp10a

Available since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pT3TS-TurboID-EGFP

Plasmid

#163846

Purpose

In vitro transcription of TurboID-EGFP. Parton lab clone JRS

Depositor

Rob Parton

Publication

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

In vitro transcription of rna

Tags

EGFP

Available since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pUAS-MEYFP

Plasmid

#86372

Purpose

membrane targeted EYFP expression in zebrafish

Depositor

Rachel Wong

Publication

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

EYFP

Use

Zebrafish

Tags

GAP43 membrane targeting

Promoter

UAS

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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nls-transposase-nls-pA in pT3TS-Dest

Plasmid

#140873

Purpose

Vector for in vitro transcription of Tol2 nls-transposase-nls for zebrafish transgenesis using T3.

Depositor

Rob Parton

Publication

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

nls-transposase-nls (Tol2)

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pME-24xMBS

Plasmid

#86246

Purpose

Middle entry vector for generating MBS tagged RNAs

Depositor

Florence Marlow

Publication

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Type

Empty backbone

Use

Unspecified

Tags

NLS-tdMCP

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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TAL3060

Plasmid

#41234

Depositor

Keith Joung

Publication

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-dazl-Left (dazl Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Dec. 14, 2012

Availability

Academic Institutions and Nonprofits only
TAL3061

Plasmid

#41235

Depositor

Keith Joung

Publication

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-dazl-Right (dazl Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only
TAL3144

Plasmid

#41318

Depositor

Keith Joung

Publication

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-pum1-Left (pum1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
TAL3145

Plasmid

#41319

Depositor

Keith Joung

Publication

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-pum1-Right (pum1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
drd4a_L (TAL 3006)

Plasmid

#35998

Depositor

Keith Joung

Publication

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting drd4a (drd4a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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p3E-TurboID

Plasmid

#166570

Purpose

Multisite Gateway 3' entry clone for fusions onto the N-terminus of TurboID. Parton lab clone JSS

Depositor

Rob Parton

Publication

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

Gateway Cloning

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR5'-attP (pCM254)

Plasmid

#68311

Purpose

Gateway middle entry vector for full-length attP site for phiC31-mediated transgenesis.

Depositor

Christian Mosimann, Leonard Zon

Publication

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Insert

attP

Use

Gateway Cloning

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2-ER-EmEGFP

Plasmid

#169000

Purpose

Express ER-EmEGFP in zebrafish

Depositor

Noboru Mizushima

Publication

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

ER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Human, Synthetic)

Use

Unspecified

Tags

EGFP

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pISceI-myl7:MBIC5-mCer3

Plasmid

#74117

Purpose

Zebrafish transgenic construct

Depositor

Michael Tsang

Publication

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MBIC5-mCer3

Use

Mrna generation for microinjection.

Tags

mCerulean 3

Expression

Mammalian

Promoter

SP6

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-kanaR-cryaa-sfGFP

Plasmid

#74153

Purpose

BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker sfGFP

Depositor

Holger Knaut

Publication

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

Tol2-kanaR-cryaa-sfGFP

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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