We narrowed to 32,912 results for: Eng
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Plasmid#222212PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the W15C mutation and a C-terminal His tagDepositorInsertfghA(W15C)
Tags6x HisExpressionBacterialMutationChanged Tryptophan 15 to CysteinePromoterT7Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
WT_IFL_G1A-T2A_R
Plasmid#200183PurposeWT PA14 I-F Leader and G1A-T2A RepeatDepositorInsertWildtype I-F Leader and a mutant I-F Locus (G1A-T2A)
ExpressionBacterialMutationG1A-T2A in the I-F repeatAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
WT_IFL_G1A-T2A-A28C_R
Plasmid#200185PurposeWT PA14 I-F Leader and G1A-T2A-A28C RepeatDepositorInsertWildtype I-F Leader and a mutant I-F Locus (G1A-T2A-A28C)
ExpressionBacterialMutationG1A-T2A-A28C in the I-F repeatAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-457
Plasmid#200213PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 H25A)DepositorInsertPaCas1, PaCas2-3
TagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 H25A mutationAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-458
Plasmid#200214PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 E184A)DepositorInsertPaCas1, PaCas2-3
TagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 E184A mutationAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-459
Plasmid#200215PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 K214A)DepositorInsertPaCas1, PaCas2-3
TagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 K214A mutationAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-460
Plasmid#200216PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 R55E, N56D)DepositorInsertPaCas1, PaCas2-3
TagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 R55E-N56D mutationsAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-461
Plasmid#200217PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E)DepositorInsertPaCas1, PaCas2-3
TagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 K11D-R12E mutationsAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-462
Plasmid#200218PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E, R55E, N56D)DepositorInsertPaCas1, PaCas2-3
TagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 K11D-R12E, R55E-N56D mutationsAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
(B) G418 marker (R) (SBE 137)
Plasmid#195011Purposegeneticin (G418) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOSDepositorInsertpXYL+G418+tNOS
ExpressionBacterialPromoterpXYLAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free NES
Plasmid#182482PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and nerolidol synthase from Actinidia chinensis (AcNES1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10 and GAL7Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free PcPTS
Plasmid#182483PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and patchoulol synthase from Pogostemon cablin (PcPTS; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
PTS
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10 and GAL7Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES (AG4TGGA)2
Plasmid#182488PurposeYeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence (AG4TGGA)2 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked Free (PT)4P
Plasmid#182490PurposeYeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence PTPTPTPTP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2326
Plasmid#169628PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
ExpressionMammalianPromoterPhCMV / PPGKAvailable SinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2321
Plasmid#169626PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
ExpressionMammalianPromoterPhCMV / PPGKAvailable SinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)_co_L-HicDH
Plasmid#185453PurposeCDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).DepositorInsertL-HicDH from Lactobacillus confusus DSM 20196
ExpressionBacterialPromoterT7Available SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2329
Plasmid#169629PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and spacer-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
ExpressionMammalianPromoterPhCMV / PPGKAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2322
Plasmid#169627PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and Tactb-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
ExpressionMammalianPromoterPhCMV / PPGKAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2320
Plasmid#169625PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and spacer-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
ExpressionMammalianPromoterPhCMV / PPGKAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2312
Plasmid#169622PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
ExpressionMammalianPromoterPhCMV / PPGKAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2317
Plasmid#169624PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
ExpressionMammalianPromoterPhCMV / PPGKAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPPC002
Plasmid#171139PurposeFor integration of J1-J23117-sfGFP, Sp.pCas9-dCas9, and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertsJ1-BBa_J23117-sfGFP
Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterJ1-BBa_J23117 and Sp.pCas9, BBa_J23107Available SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKdH_P-GAP-ScACS1*
Plasmid#126736Purposeyeast plasmid overexpressing ACS1 from Saccharomyces cerevisiaeDepositorInsertacs1*
Tags6xHis,MycExpressionYeastMutationT3542C and A30S, P113S, and S629N (please see dep…PromoterGAPAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKdH_P-GAP-ScADH2+P-GAP*-ScACS1*
Plasmid#126738Purposeyeast plasmid overexpressing ADH2 and ACS1 from Saccharomyces cerevisiaeDepositorInsertadh2,acs1*
Tags6xHis,MycExpressionYeastMutationT3542CPromoterGAPAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKdH_P-GAP-ScADH2+P-GAP*-ScALD6
Plasmid#126737Purposeyeast plasmid overexpressing ADH2 and ALD6 from Saccharomyces cerevisiaeDepositorInsertadh2,ald6
Tags6xHisExpressionYeastPromoterGAPAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-ICL1-LacI-Mit1AD
Plasmid#126721PurposeLacI-Mit1AD on pPICZ B expression vectorDepositorInserthybrid lacI-Mit1 activation domain coding sequence
ExpressionYeastPromoterICL1Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-ICL1-LacI-VP16
Plasmid#126723PurposeLacI-VP16 on pPICZ B expression vectorDepositorInserthybrid lacI-VP16 activation domain coding sequence
ExpressionYeastPromoterICL1Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-ICL1-LacI-Gal4AD
Plasmid#126724PurposeLacI-Gal4AD on pPICZ B expression vectorDepositorInserthybrid lacI-Gal4 activation domain coding sequence
ExpressionYeastPromoterICL1Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-sLovA
Plasmid#126741Purposeyeast plasmid overexpressing sLovADepositorInsertslovA from Aspergillus terreus ATCC 20542 (LovA) codon optimized for expression in Komagataella phaffii
Tags6xHisExpressionYeastAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only