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ID1GFP Donor

Plasmid

#119282

Purpose

Mouse ID1-eGFP for HR to create reporter

Depositor

Joan Massague

Article

Huang et al Cancer Discov. 2019 Oct 3. pii: 2159-829

Insert

Id1GFP + homology arms (Id1 Mouse)

Use

Reporter

Tags

eGFP

Expression

Mutation

Promoter

Available since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-GFP-shCrh2

Plasmid

#132710

Purpose

Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Crh gene

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

shCrh(2) (Crh Rat)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-Stx3 L165A/E166A-2xMyc/His

Plasmid

#99743

Purpose

Expresses human Stx3 locked into the open conformation with a C-term myc-myc-his tag in mammalian cells.

Depositor

Thomas Weimbs

Article

Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

Insert

Stx3 (STX3 Human)

Use

Tags

myc-myc-his

Expression

Mammalian

Mutation

Changed both L165 and E166 to Alanine

Promoter

CMV

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pSN17

Plasmid

#100104

Purpose

expresses a chimeric OCT4-repressor fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to full length HP1 alpha (POU5F1, CBX5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLOX-gfp-iresTK

Plasmid

#12243

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

GFP, HSV1-TK

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

LoxP in 3' LTR.

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pISH-Msx1

Plasmid

#84313

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Msx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Msx1 (Msx1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pISH-Otx1

Plasmid

#84315

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Otx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Otx1 (Otx1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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GST-PRMT2

Plasmid

#34650

Depositor

Steven Clarke

Article

Miranda et al Biochem Biophys Res Commun. 2005 Oct 28;

Insert

PRMT2 (PRMT2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
EC10161

Plasmid

#154060

Purpose

Level 0.5 Golden Gate backbone, containing loxP sites.

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

N/A

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCIneo-RL-5BoxB

Plasmid

#115364

Purpose

Expression vector to produce humanized Renilla luciferase with 5BoxB sequences in the 3'UTR

Depositor

Ramesh Pillai, Witold Filipowicz

Article

Pillai et al RNA. 2004 Oct . 10(10):1518-25.

Insert

Renilla Luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PM-GFP

Plasmid

#21213

Depositor

Tobias Meyer

Article

Teruel et al J Neurosci Methods. 1999 Oct 30. 93(1):3

Insert

PM-GFP

Use

Tags

GFP

Expression

Mammalian

Mutation

PM targetting sequence is Lyn (MGCIKSKRKD) (Lab …

Promoter

Available since

July 13, 2009

Availability

Academic Institutions and Nonprofits only

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pCFD7

Plasmid

#73916

Purpose

gRNA vector for AsCpf1

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

tRNA:hAsCpf1-gRNA:tRNA

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

dU6:3

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pLOX-CWgfp

Plasmid

#12241

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

eGFP

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

CMV promoter is upstream of GFP. LoxP in 3' …

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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Flag-FOXO3B

Plasmid

#153153

Purpose

Expression of Flag tagged human FOXO3B in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXO3B (FOXO3B Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV IE95

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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HT-mGold

Plasmid

#183986

Purpose

Expresses fluorescent HaloTag7 fusion protein in mammalian cells in the whole cell.

Depositor

Pablo Rivera-Fuentes

Article

Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

Insert

HaloTag-mGold

Use

Tags

HaloTag, mGold

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLOX-IWgfp

Plasmid

#12244

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

eGFP

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter of the Rat Insulin II gene is upstream o…

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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Flag-FOXD4L5

Plasmid

#153156

Purpose

Expression of Flag tagged human FOXD4L5 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD4L5 (FOXD4L5 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV IE98

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pDL14

Plasmid

#17837

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

gpdA promoter, lacZ, trpC

Use

Synthetic Biology; A. nidulans

Tags

Expression

Mutation

Promoter

Available since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
p3EHA

Plasmid

#67708

Purpose

3' entry HA tag

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

HA tag

Use

Tags

HA

Expression

Mutation

Promoter

None

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pISH-Trpm5

Plasmid

#105993

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Trpm5 (Trpm5 Mouse)

Use

To generate riboprobe

Tags

Expression

Mutation

Promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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