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pAL725
Plasmid#119862Purposeprecusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287CDepositorInsertD-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
UseTagsnoneExpressionBacterialMutationLeucine 244 to Cysteine, Aspartate 287 to CysteinePromoterT5Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLIPE-10u
Plasmid#13542DepositorInsertFLIP-E A207R
UseTagsECFP and VenusExpressionMammalianMutationA207R in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDisplay FLIPE-1m
Plasmid#13548DepositorInsertFLIP-E A207W
UseTagsECFP and VenusExpressionMammalianMutationA207W in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDisplay FLIPE-10u
Plasmid#13546DepositorInsertFLIP-E A207R
UseTagsECFP and VenusExpressionMammalianMutationA207R in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B Flag BimEL(TD)
Plasmid#23092DepositorInsertBim EL (TD) (Bcl2l11 Mouse)
UseTagsFlagExpressionMammalianMutationChanged Threonine 112 to AspartatePromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pYes2-AtPCS1
Plasmid#49766PurposeExpresses Arabidopsis phytochelatin synthase (AtPCS1) in Saccharomyces CerevisiaeDepositorInsertAtPCS1 (PCS1 Mustard Weed)
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQE80L SpyTag-ELP-SpyTag DK
Plasmid#112635PurposeSpyTag linked by an elastin-like polypeptide to non-reactive SpyTag DKDepositorInsertSpyTag-ELP-SpyTag DK
UseTagsHis6ExpressionBacterialMutationContains central cysteine. Reactive aspartic acid…PromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDisplay FLIPE-100u
Plasmid#13547DepositorInsertFLIP-E S118W
UseTagsECFP and VenusExpressionMammalianMutationS118W in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Hemmar-FLP(D)
Plasmid#119895PurposeFLP(D) with a hsp70 promoterDepositorInsertFLP(D)
UseDrosophila expressionTagsExpressionMutationmutated form of FLP, which at position 5 contains…PromoterAvailable sinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest53-SHP-1 S591D
Plasmid#21803DepositorInsertSHP-1 (PTPN6 Human)
UseTagsGFPExpressionMammalianMutationchanged Serine 591 to Aspartic acidPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPE intramol-1m
Plasmid#13551DepositorInsertFLIP-E intramol A207W (gltI E. coli)
UseTagsEYFPExpressionBacterialMutationA207W, ECFP insert in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-rbN1 S77D
Plasmid#32947DepositorInsertslc9a3r1 (SLC9A3R1 rabbit)
UseTagsT7 tag, his, and partial FLAGExpressionMammalianMutationSerine 77 mutated to Aspartic AcidPromoterCMVAvailable sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-I70V/D91G
Plasmid#47644PurposepAC-EsaR with a208g and a272g mutations in esaRDepositorInsertesaR-I70V/D91G
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Isoleucine 70 to Valine and Aspartic acid…PromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGSTag BimEL (T112D)
Plasmid#23102DepositorInsertBim EL (TD) (Bcl2l11 Mouse)
UseTagsGstExpressionBacterialMutationChanged Threonine 112 to AspartatePromoterAvailable sinceFeb. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPE intramol-10u
Plasmid#13550DepositorInsertFLIP-E intramol A207R (gltI E. coli)
UseTagsEYFPExpressionBacterialMutationA207R, ECFP insert in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
TEG +H122D
Plasmid#187434PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged histidine at 128 to aspartic acidPromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
FER +H122D
Plasmid#187428PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged histidine at 128 to aspartic acidPromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
MON +N122H
Plasmid#187433PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged asparagine at 128 to histidinePromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCC_084 Cas12i1 D647A
Plasmid#171671PurposeFor bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertCas12i1 D647A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 647 to alaninePromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only
