We narrowed to 85,205 results for: Mal;

1. pEGFP-Rab21-T31N (DN)

   Plasmid

   #83423

   Purpose

   Mouse Rab21 mutant that has defects in the binding of GTP (dominant negative). Generated from pEGFP-Rab21 by site-directed mutagenesis.

   Depositor

   Johanna Ivaska

   Article

   Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

   Insert

   Rab21 (Rab21 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Residue 31 from T to N (T31N: ACG -> AAC)

   Promoter

   CMV

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SpCas9 TadCBEa

   Plasmid

   #193782

   Purpose

   Express TadCBEa (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDa-SpCas9D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-ITR-PytR-mCherry

   Plasmid

   #204868

   Purpose

   Expresses pyrrolysyl tRNA and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   M. mazei pyrrolysyl tRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   U25C

   Promoter

   U6

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEF1a-Usp9x_CD-mCherry

   Plasmid

   #170380

   Purpose

   Vector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.

   Depositor

   Miguel Ramalho-Santos

   Article

   Macrae et al Nat Commun. 2021 Mar 25;12(1):1865. doi:

   Insert

   Ubiquitin specific protease 9x catalytic domain (Usp9x Mouse)
   

   Use

   Tags

   mCherry-3xFlag

   Expression

   Mammalian

   Mutation

   Catalytic domain

   Promoter

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

   Plasmid

   #200910

   Purpose

   3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

   Depositor

   Debojyoti Chakraborty

   Insert

   3XHA_den1FnCas9_KRAB_eGFP
   

   Use

   CRISPR

   Tags

   3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP

   Expression

   Mammalian

   Mutation

   Changed Glutamic Acid to Arginine at 1369 position

   Promoter

   CBH

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AsCas12a-2C-NLS in pCSDest

   Plasmid

   #126637

   Purpose

   Expresses AsCas12a-2C-NLS in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   AsCas12a-2C-NLS
   

   Use

   CRISPR

   Tags

   3xHuman influenza hemagglutinin (HA)-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94 promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SpCas9 TadCBEc

   Plasmid

   #193834

   Purpose

   Express TadCBEc (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDc-SpCas9 D10A-2xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-hSyn-Flex-mRuby3-2A

   Plasmid

   #203846

   Purpose

   Neuron specific expression of a Cre-dependent mRuby3

   Depositor

   Ivo Spiegel

   Article

   Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

   Insert

   mRuby3
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SaCas9 TadCBEa

   Plasmid

   #193844

   Purpose

   Express TadCBEa (with SaCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   SaCas9 TadCBEa
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NET5s pEGFP-N2 (1154)

    Plasmid

    #62034

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET5s (TMEM201 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    short form

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pEBTet-SNAP-CCCAP

    Plasmid

    #136798

    Purpose

    Mammalian expression of the centrosomal protein CCCAP N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-CCCAP (SDCCAG8 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-Rab21-CCSS

    Plasmid

    #83424

    Purpose

    Mouse Rab21, mutation of the putative COOH-terminal prenylation motif (residues 218 and 219 from C to S). Generated from pEGFP-Rab21 by site-directed mutagenesis.

    Depositor

    Johanna Ivaska

    Article

    Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

    Insert

    Rab21 (Rab21 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Residues 218 and 219 from C to S (CCSS: TGCTGT -&…

    Promoter

    CMV

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRetro-XT-PAK2-CFP(puro)-WT

    Plasmid

    #81176

    Purpose

    Retroviral vector for expression of wild-type PAK2 with a CFP fusion

    Depositor

    Angeliki Malliri

    Article

    Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

    Insert

    p21 (RAC1) activated kinase 2 (PAK2 Human)
    

    Use

    Retroviral

    Tags

    CFP

    Expression

    Mutation

    Promoter

    P-tight TRE

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pRetro-XT-PAK2-CFP(puro)-T402A

    Plasmid

    #81179

    Purpose

    Retroviral vector for expression of kinase inactive PAK2 with a CFP fusion

    Depositor

    Angeliki Malliri

    Article

    Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

    Insert

    p21 (RAC1) activated kinase 2 (PAK2 Human)
    

    Use

    Retroviral

    Tags

    CFP

    Expression

    Mutation

    T402A

    Promoter

    P-tight TRE

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEBTet-SNAP-Sfi1

    Plasmid

    #136793

    Purpose

    Mammalian expression of the centrosomal protein hSfi1 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-hSfi1 (SFI1 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FLAG-MED29

    Plasmid

    #15409

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    MED29 (MED29 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

17. FnoCas12a-2C-NLS in pCSDest

    Plasmid

    #126639

    Purpose

    Expresses FnoCas12a-2C-NLS in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    FnoCas12a-2C-NLS
    

    Use

    CRISPR

    Tags

    3xHuman influenza hemagglutinin (HA)-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94 promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRetro-XT-PAK2-CFP(puro)-L107F

    Plasmid

    #81180

    Purpose

    Retroviral vector for expression of kinase active PAK2 with a CFP fusion

    Depositor

    Angeliki Malliri

    Article

    Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

    Insert

    p21 (RAC1) activated kinase 2 (PAK2 Human)
    

    Use

    Retroviral

    Tags

    CFP

    Expression

    Mutation

    L107F

    Promoter

    P-tight TRE

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pSBL_DK202

    Plasmid

    #156426

    Purpose

    Mammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein (aa 42-275)
    

    Use

    Tags

    EGFP-His

    Expression

    Mammalian

    Mutation

    aa 42-275

    Promoter

    CMV

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SpCas9 TadCBEb

    Plasmid

    #193832

    Purpose

    Express TadCBEb (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDb-SpCas9 D10A-2xUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge