-
Plasmid#188944PurposeMiddle entry vector for Gateway cloning containing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherryDepositorInsertmito-APEX2_p2A_APEX2-H2B_p2A_mCherry
UseMiddle entry vector for gateway (l1-l2)TagsExpressionMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXW109Hg(RS-RinA)
Plasmid#123150PurposeMercury sensor with two-layered amplifier cascade. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, PmerT, PhrpLE, PrinAAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXW109ABS84(RS)
Plasmid#123155PurposeAmplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, ParsR-ABS84, PhrpLEAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
STAGR_SAMScaffold_h7SK
Plasmid#102846PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert SAM_h7SK
UsePcr template for stagr insertsTagsExpressionMutationPromoterH7SKAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH124
Plasmid#48259PurposeTemplate plasmid for PCR-based transplant of Ashbya gossypii TEF1 promoter in yeast.DepositorInsertsURA3 3' fragment
URA3 5' fragment
Ag TEF1 promoter
UseRoutine cloning vectorTagsExpressionMutation-242 upstream to URA3 ORF +495 and URA3 ORF from …PromoterAg TEF1 promoterAvailable sinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-Z3-Nat-ADE2
Plasmid#232106PurposeExpresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterZ3Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-Z3-Hyg-ADE2
Plasmid#232107PurposeExpresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterZ3Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
-
pUC57-min5UTR-hRluc-A60
Plasmid#182638PurposeTranscription template plasmid for a minimal 5'UTR followed by Renilla luciferase open reading frame and polyA sequenceDepositorInsertRenilla luciferase,
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ATG101
Plasmid#189590PurposeTo make ATG101/ATG13 HORMA complexDepositorInsertATG101 (ATG101 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXW109ABS62(RS)
Plasmid#123153PurposeAmplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS62-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS62-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, ParsR-ABS62, PhrpLEAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXW109ABS67(RS)
Plasmid#123154PurposeAmplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109. ParsR-ABS67, PhrpLEAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
HIV-1 LTR-gfp
Plasmid#115809PurposeHIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vprDepositorInsertgfp and ngfr
UseLentiviralTagsExpressionMutationPromoterHIV1 LTRAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTargeting_Mettl3C_FKBP-V
Plasmid#165421PurposeTemplate for knockin FBKP-V into Mettl3 C-terminusDepositorInsertMettl3 C-terminus + linker + FKBP-V (Mettl3 Mouse)
UseMouse TargetingTagsFKBP-VExpressionMutationPromoterAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Nat-ADE2
Plasmid#232103PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-BECN1
Plasmid#99328PurposeComponent for mammalian expression of recombinant PI3KC3-C1 complexDepositorInsertBeclin 1 (BECN1 Human)
UseTagsStrep-Strep-FlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-VPS34
Plasmid#99327PurposeComponent for mammalian expression of recombinant PI3KC3-C1 complexDepositorInsertVPS34 (PIK3C3 Human)
UseTagsStrep-Strep-FlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
p21 shRNA1
Plasmid#25868DepositorInsertp21 (Cdkn1a Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
His-MBP-NRBF2
Plasmid#99330PurposeMammalian expression of NRBF2, which associates with the PI3KC3-C1 complexDepositorInsertNuclear receptor-binding factor 2 (NRBF2 Human)
UseTagsHis-MBPExpressionBacterialMutationPromoterT7Available sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
p21 shRNA2
Plasmid#25869DepositorInsertp21 (Cdkn1a Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only