We narrowed to 1,571 results for: Tef;

1. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213029

   Purpose

   Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)

   Plasmid

   #209078

   Purpose

   Ngn2 mediated cortical neuron induction, along with SNCA(3K) over-expression

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   SNCA (SNCA Human)
   NGN2 (Neurog2 Mouse)
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   3K mutant: E35K, E46K, E61K

   Promoter

   TRE4G

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

   Plasmid

   #209080

   Purpose

   Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   SNCA (SNCA Human)
   NGN2 (Neurog2 Mouse)
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   A53T

   Promoter

   TRE4G

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSrTA1662-toolkit

   Plasmid

   #154372

   Purpose

   Expresses SrTA1662 in plants

   Depositor

   Brande Wulff

   Article

   Gaurav et al Nat Biotechnol. 2022 Mar;40(3):422-431.

   Insert

   SrTA1662
   

   Use

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Native promoter

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (VK_1124)

   Plasmid

   #209081

   Purpose

   Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-DeltaNAC-sfGFP over-expression

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   SNCA (SNCA Human)
   NGN2 (Neurog2 Mouse)
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   A53T, NAC deletion (aa60-95)

   Promoter

   TRE4G

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETTEV-SENP8

   Plasmid

   #196361

   Purpose

   protein expression of the His-tagged full length protein

   Depositor

   Stefan Knapp

   Insert

   SENP8/NEDP1
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNIC28-BIRC7

   Plasmid

   #196363

   Purpose

   protein expression of the His-tagged full length protein

   Depositor

   Stefan Knapp

   Insert

   BIRC7/Livin
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNIC28-BIRC3

   Plasmid

   #196366

   Purpose

   protein expression of the His-tagged full length protein

   Depositor

   Stefan Knapp

   Insert

   BIRC3/cIAP2
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pILFPZ1A9

   Plasmid

   #223835

   Purpose

   Expressing a cytosolic pH sensor (mTagBFP2-mNeonGreen) in Saccharomyces cerevisiae

   Depositor

   Claudia Vickers

   Insert

   pTEF1>mTagBFP>mNeonGreen>
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pILFPQ13BE5

    Plasmid

    #223836

    Purpose

    Expressing a cytosolic cAMP sensor (mScarletI3-ApacH-StaryGold2) in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Insert

    pTEF1>mScarletI3>ApacH>StayGold2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKM263-USP7

    Plasmid

    #196365

    Purpose

    protein expression of the GST-tagged catalytic domain

    Depositor

    Stefan Knapp

    Insert

    USP7 catalytic domain
    

    Use

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    contains only S207-E560

    Promoter

    T7

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNIC28-SENP2

    Plasmid

    #196355

    Purpose

    protein expression of the His-tagged catalytic domain

    Depositor

    Stefan Knapp

    Insert

    SENP2 catalytic domain
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    contains only D364-L589

    Promoter

    T7

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGTVL2-SENP6

    Plasmid

    #196359

    Purpose

    protein expression of the GST-tagged catalytic domain

    Depositor

    Stefan Knapp

    Insert

    SENP6 catalytic domain
    

    Use

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    contains only E652-A1095

    Promoter

    T7

    Available since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKM263-USP40

    Plasmid

    #196356

    Purpose

    protein expression of the GST-tagged catalytic domain

    Depositor

    Stefan Knapp

    Insert

    USP40 catalytic domain
    

    Use

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    contains only R35-Q383

    Promoter

    T7

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGTVL2-SENP3

    Plasmid

    #196357

    Purpose

    protein expression of the GST-tagged catalytic domain

    Depositor

    Stefan Knapp

    Insert

    SENP3 catalytic domain
    

    Use

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    contains only S307-T573

    Promoter

    T7

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNIC28-SENP1

    Plasmid

    #196354

    Purpose

    protein expression of the His-tagged catalytic domain

    Depositor

    Stefan Knapp

    Insert

    SENP1 catalytic domain
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    contains only E419-L644

    Promoter

    T7

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGTVL2-SENP5

    Plasmid

    #196358

    Purpose

    protein expression of the GST-tagged catalytic domain

    Depositor

    Stefan Knapp

    Insert

    SENP5 catalytic domain
    

    Use

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    contains only S492-D755

    Promoter

    T7

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKM263-Rpn13

    Plasmid

    #196360

    Purpose

    protein expression of the GST-tagged PRU domain

    Depositor

    Stefan Knapp

    Insert

    Rpn13 PRU domain
    

    Use

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    contains only T2-L150

    Promoter

    T7

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJY4092

    Plasmid

    #113334

    Purpose

    Vector for Hermes transposition; Hermes TIR-flanked AvrII cut site; recoded transposase driven by TEF1 promoter

    Depositor

    Suzanne Sandmeyer

    Insert

    codon-optimized Hermes transposase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSpliceExpress

    Plasmid

    #32485

    Depositor

    Stefan Stamm

    Article

    Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge