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pAS1-gpa-2P::his-24::Cerulean

Plasmid

#124337

Purpose

Multicolor Worms for Neuronal ID

Depositor

Andrew Leifer

Insert

gpa-2, Cerulean (gpa-2 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-lim-4P::his-24::Cerulean

Plasmid

#124336

Purpose

Multicolor Worms for Neuronal ID

Depositor

Andrew Leifer

Insert

lim-4, Cerulean (lim-4 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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PP-G6PI

Plasmid

#28283

Depositor

Cheryl Arrowsmith

Insert

PP-G6PI (PF14_0341 Plasmodium falciparum)

Use

Tags

His tag with TEV cleavage

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pAS1-odr-4P::his-24::mNeptune

Plasmid

#124342

Purpose

Multicolor Worms for Neuronal ID

Depositor

Andrew Leifer

Insert

odr-4, mNeptune (odr-4 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-tax-4P::his-24::mOrange

Plasmid

#124340

Purpose

Multicolor Worms for Neuronal ID

Depositor

Andrew Leifer

Insert

tax-4, mOrange (tax-4 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pRARB.1.0-gDNA

Plasmid

#132471

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Kevin White

Insert

RARB (RARB Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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2QQ8

Plasmid

#60110

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

TBC1D14:TBC1D14_dml-X01:C201000

Use

Tags

6xHis

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
4JUY

Plasmid

#60083

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

RNF31:YTC010-F07:C227920

Use

Tags

6xHis

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
SIRT5

Plasmid

#25487

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

SIRT5 (SIRT5 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 23, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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4MX2

Plasmid

#60102

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

LdBPK_040440.1:MAC04A-B07:C226244

Use

Tags

6xHis

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pCAMTA2.1.0-gDNA

Plasmid

#132456

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Kevin White

Insert

CAMTA2 (CAMTA2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMBD1.1.0-gDNA

Plasmid

#132444

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Kevin White

Insert

MBD1 (MBD1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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6NIM

Plasmid

#127294

Purpose

Bacterial expression for structure determination. May not contain entire coding region of gene

Depositor

Cheryl Arrowsmith

Insert

PP-BRD18

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 26, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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4QNY

Plasmid

#61958

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

LdBPK_331470.1:MAC04E-A10:C228347

Use

Tags

MHHHHHHSSGRENLYFQG

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available since

March 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits
2P65

Plasmid

#60113

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

Pv089580:MAC01Q-D10:C26843

Use

Tags

6xHis

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
4JWQ

Plasmid

#60084

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

PB000947.00.0:MAC03Z-H05:C218239

Use

Tags

6xHis

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
4LDF

Plasmid

#60089

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

cgd3_3190:MAC04H-E05:C228722

Use

Tags

6xHis

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
4LDG

Plasmid

#60090

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

cgd4_370:MAC04H-G03:C228753

Use

Tags

6xHis

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pUltraHot-mCherry-DHX36-iso1-WT

Plasmid

#206964

Purpose

expressing the protein DHX36 fused to mCherry in human cells

Depositor

Eran Hornstein

Article

Danino et al Nucleic Acids Res. 2023 Jul 28:gkad613.

Insert

DEAH-Box Helicase 36 (DHX36 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pARC8-LambdaRedBeta-EcSSB-EcMutLE32K

Plasmid

#162576

Purpose

Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.

Depositor

George Church

Article

Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038

Inserts

Lambda Red-Beta
E. coli SSB
EcMutLE32K

Use

Tags

Expression

Bacterial

Mutation

E32K mutated to give dominant negative phenotype

Promoter

pBAD

Available since

May 22, 2021

Availability

Academic Institutions and Nonprofits only

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