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pZac2.1 gfaABC1D-BioID2-BioID2-Kir4.1-HA

Plasmid

#176746

Purpose

Expresses Kir4.1 fused with BioID2 at the N-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

HA-BioID2-BioID2-Kir4.1 (Kcnj10 Aquifex aeolicus, Rat)

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGIGYF_AH

Plasmid

#148918

Purpose

Insect Expression of DmGIGYF

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-HsNot9_19-285_AF

Plasmid

#148733

Purpose

Bacterial Expression of HsNot9_19-285

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot9_19-285 (CNOT9 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot9_18-285_S

Plasmid

#147480

Purpose

Mammalian Expression of HsNot9_18-285

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9_18-285 (CNOT9 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsXRN1_1535-1706_K

Plasmid

#146791

Purpose

Mammalian Expression of HsXRN1_1535-1706

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

HsXRN1_1535-1706 (XRN1 Human)

Expression

Mammalian

Mutation

one non silent mutation S1688P compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-TEV-HsRCK_296-472_E

Plasmid

#146209

Purpose

Bacterial Expression of HsRck_296-472. Contains a TEV site rather than a HRV 3C site downstream of NusA.

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsRck_296-472 (DDX6 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(sapI)-CMV-intron-hfCas13d-pA

Plasmid

#195865

Purpose

To express hfCas13d

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

high fidelity (hf) version of CasRx (hfCas13d)

Use

AAV

Mutation

Yes: GTGGAATACATTACCAACGTGGTGTACGTG

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP2-F404AF407AF409A_V

Plasmid

#147813

Purpose

Mammalian Expression of HsDCP2-F404AF407AF409A

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP2-F404AF407AF409A (DCP2 Human)

Expression

Mammalian

Mutation

two silent mutations compared to the sequence giv…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3-DCX-TurboID-V5

Plasmid

#190740

Purpose

Mammalian expression of DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

Depositor

Jeremy Baskin

Article

Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

Insert

doublecortin (DCX Human)

Tags

TurboID and V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFA5-mVenus

Plasmid

#185646

Purpose

NDUFA5 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA5 (NDUFA5 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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3xNLS-NLP-cMyc-cMyc LbaCas12a

Plasmid

#182125

Purpose

pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

3xNLS-NLP-cMyc-cMyc LbaCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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3xNLS-NLP-cMyc-cMyc enAspCas12a

Plasmid

#182127

Purpose

pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

3xNLS-NLP-cMyc-cMyc enAspCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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3xNLS-NLP-cMyc-cMyc AspCas12a

Plasmid

#182122

Purpose

pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

3xNLS-NLP-cMyc-cMyc AspCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-GFP11-RhoA

Plasmid

#181860

Purpose

Part of FluoSTEP-RhoA biosensor; co-express with cpPKN-mRuby2-GFP1-10 (Addgene #181859).

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

GFP11-RhoA (RHOA Human)

Tags

GFP11

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0160

Plasmid

#172812

Purpose

Level 1 module containing P19_P1 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CDS1_P19-3U+TERNOS

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF385A

Plasmid

#101615

Purpose

Donor Vector containing ZNF385A transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF385A (ZNF385A Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6Biso3_C

Plasmid

#146088

Purpose

Mammalian Expression of HsTNRC6Biso3

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6Biso3 (TNRC6B Human)

Expression

Mammalian

Mutation

AA 3-188 and AA residues 242-1029 (with N242D) co…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCup_C

Plasmid

#146041

Purpose

Insect Expression of DmCup

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmCup (cup Fly)

Expression

Insect

Mutation

P418S

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-KR-Linker-eGFP

Plasmid

#176066

Purpose

EGFP fused to the C-terminus of a KR domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

KR

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 UBXN11

Plasmid

#169017

Purpose

Expresses GST-UBXN11 (human) in bacteria

Depositor

Hemmo Meyer

Article

Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

Insert

UBXN11 (UBXN11 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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