We narrowed to 10,347 results for: plasmids 101

1. v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

   Plasmid

   #207860

   Purpose

   AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   NpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
   

   Use

   AAV

   Tags

   Expression

   Mutation

   See Manuscript

   Promoter

   Cbh

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. MYC_pLX307

   Plasmid

   #98363

   Purpose

   Lentiviral expression of MYC

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   MYC (MYC Human)
   

   Use

   Lentiviral

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. v3em-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

   Plasmid

   #207861

   Purpose

   AAV genome encoding C-terminal PE6c and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   NpuC-Cterm-PE6c-dualU6-Rosa26-twinPE-attB
   

   Use

   AAV

   Tags

   Expression

   Mutation

   See Manuscript

   Promoter

   Cbh

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_FKBP-V5-ALX1

   Plasmid

   #215023

   Purpose

   AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human ALX1

   Depositor

   Joanna Wysocka

   Article

   Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

   Insert

   ALX homeobox 1 (ALX1 Human)
   

   Use

   AAV

   Tags

   FKBP-V5

   Expression

   Mutation

   Promoter

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-BEC enhancer-minBG-iCre(R279T)-4x6T

   Plasmid

   #236727

   Purpose

   Brain Endothelial Cell Enhancer with 4x6T miRNA targeting cassette For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors.

   Depositor

   Xiangmin Xu

   Article

   Velazquez-Rivera et al Neuron. 2025 May 21;113(10):1562-1578.e6

   Inserts

   cis-regulatory elements 89763
   iCre(R297T)
   4x6T miRNA targeting cassette
   

   Use

   AAV

   Tags

   Expression

   Mutation

   R297T

   Promoter

   Available Since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGEN-Vhh-LaminB-mNeonGreen-IRES-myr-BFP (JDW 1353)

   Plasmid

   #229845

   Purpose

   A CAGGS-driven expression vector containing a vhh-Lamin nanobody for nuclear lamina labeling followed by an IRES and a myristoylated TagBFP for labeling the cell membrane.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Lamin nanobody
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP

   Plasmid

   #207863

   Purpose

   AAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   NpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   See Manuscript

   Promoter

   Cbh

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. 6-His-MBP-TEV-FnCpf1

   Plasmid

   #90094

   Purpose

   Bacterial expression plasmid for protein purification

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   FnCpf1 (humanized)
   

   Use

   Tags

   3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. TGFB1_pLX307

   Plasmid

   #98377

   Purpose

   Lentiviral expression of TGFB1

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   TGFB1 (TGFB1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR

    Plasmid

    #207805

    Purpose

    E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.

    Depositor

    Koen Breyne

    Article

    Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. do

    Insert

    E-NoMi
    

    Use

    Lentiviral

    Tags

    3xFlag tag, copGFP, mCherry, and nanoluciferase

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLIK-IDH1-FLAG

    Plasmid

    #66802

    Purpose

    Tet-inducible expression of IDH1 in mammalian cells, 3rd generation lentiviral vector

    Depositor

    Christian Metallo

    Article

    Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. TetO-FUW-NICD

    Plasmid

    #61540

    Purpose

    Expresses NOTCH-ICD in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    NOTCH-ICD (NOTCH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    NOTCH intracellular domain (aa1761-2555); A1943S…

    Promoter

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRE-UBC-hCD59-T2A-GFP

    Plasmid

    #205460

    Purpose

    lentiviral plasmid for expression of human CD59

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    hCD59 (CD59 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLIK-IDH2-R172K-FLAG

    Plasmid

    #66807

    Purpose

    Tet-inducible expression of mutant IDH2 in mammalian cells

    Depositor

    Christian Metallo

    Article

    Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

    Insert

    IDH2 (IDH2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R172K

    Promoter

    CMV

    Available Since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Psicheck Cdk2ap1Exon1

    Plasmid

    #178028

    Purpose

    expression vector to test translation efficiency by luciferase reporter.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Luciferase; Nonviral

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Cloned in

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. CDK9_pLX307

    Plasmid

    #98324

    Purpose

    Lentiviral expression of CDK9

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    CDK9 (CDK9 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX304-YAP1(S94A)

    Plasmid

    #59145

    Purpose

    YAP1 serine 94 o alanine mutants which disrupts binding to TEAD transcription factors

    Depositor

    William Hahn

    Article

    Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S94A

    Promoter

    CMV

    Available Since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cbh_v5 AAV-saCBE_KKH C-terminal

    Plasmid

    #137184

    Purpose

    AAV genome: expresses the C-terminal of S. aureus v5 AAV-CBE, and U6-sgRNA (KKH variant).

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-saCBE_KKH C-terminal
    

    Use

    AAV

    Tags

    Expression

    Mutation

    E782K;N968K;R1015H conferring recognition of NNNR…

    Promoter

    Cbh

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. SPACE (pRZ1813)

    Plasmid

    #140242

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V82G in TadA*, D10A in Cas9, R187W in pmCDA1

    Promoter

    CMV

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETDuet-1_6xHis-TEV-mCh-NDP52

    Plasmid

    #187829

    Purpose

    Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Tags

    6xHisTag, TEV, and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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