We narrowed to 17,258 results for: tes

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pLKO.1-shCELF4-4405

Plasmid

#115466

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Publication

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shCELF4-4405 (CELF4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCELF4-4404

Plasmid

#115465

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Publication

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shCELF4-4404 (CELF4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST-Tol2(Chien)-Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA

Plasmid

#105643

Purpose

Co-expression of tdTomato and HyPer in differentiated keratinocytes of the periderm layer and ealy on also in the basal layer of the epidermis

Depositor

Sandra Rieger

Publication

Lisse et al Proc Natl Acad Sci U S A. 2016 Apr 12;11

Insert

Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA

Expression

Mammalian

Available since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3.1+_FH-AGO2-5xA

Plasmid

#92007

Purpose

Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

Depositor

Joshua Mendell

Publication

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824A, S828A, T830A, S831A, S834A (5xA)

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-D358K

Plasmid

#91983

Purpose

Expresses FLAG-HA-AGO2 (D358K)

Depositor

Joshua Mendell

Publication

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

D358K

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-K525E

Plasmid

#91985

Purpose

Expresses FLAG-HA-AGO2 (K525E)

Depositor

Joshua Mendell

Publication

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

K525E

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-CCL5.Pro

Plasmid

#79492

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) CCL5/RANTES promoter

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

CCL5 Promoter (CCL5 Synthetic, Human)

Use

Gateway Cloning

Promoter

CCL5 Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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myc-Atg14 S29D

Plasmid

#86757

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Publication

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

atg14 (ATG14 Human)

Tags

myc

Expression

Mammalian

Mutation

S29D

Promoter

CMV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pILGFPH7

Plasmid

#83554

Purpose

Used to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Publication

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

ADH2 promoter-yEGFP

Expression

Yeast

Promoter

ADH2

Available since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCALNL-eGFP-NT1-6-gix-6-NT1

Plasmid

#81204

Purpose

contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid

Depositor

David Liu

Publication

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

gix-Neo-gix deletion cassette upstream of EGFP

Expression

Mammalian

Promoter

pCBa

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2-MT-mitfa

Plasmid

#81231

Purpose

expression of zebrafish mitfa

Depositor

James Lister, David Raible

Publication

Lister et al Development. 1999 Sep;126(17):3757-67.

Insert

mitfa (mitfa Zebrafish)

Use

Zebrafish expression

Tags

6 myc tags

Expression

Mammalian

Mutation

contains amino acids 5-412

Promoter

sCMV IE94 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-alpha4 wt (Hygro)

Plasmid

#80017

Purpose

Mammalian expression of human integrin alpha4, Hygro selection

Depositor

Chinten James Lim

Publication

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

integrin alpha4 (ITGA4 Human)

Expression

Mammalian

Promoter

CMV

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
GST-PS-Grb2 1-154 (XSB382)

Plasmid

#78510

Purpose

Construct for expressing Grb2 lacking the C-terminal SH3 domain in E. Coli

Depositor

Ron Vale

Publication

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

Grb2 1-154 (GRB2 Human)

Tags

GST followed by a prescission protease claevage s…

Expression

Bacterial

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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YFP(1-158) in pcDNAI/Amp

Plasmid

#60888

Purpose

This vector attaches YFP(1-158) to the N-terminus of a protein.

Depositor

Catherine Berlot

Publication

Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

Insert

YFP(1-158)

Expression

Mammalian

Mutation

Met was substituted for Gln-69.

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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NET33 pmRFP-N2 (448)

Plasmid

#61979

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Publication

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NET33 (SCARA5 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pcdna3 T7 Jip1b

Plasmid

#52122

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Publication

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)

Tags

T7

Expression

Mammalian

Available since

April 28, 2014

Availability

Academic Institutions and Nonprofits only
pMM2-rPMCA3b

Plasmid

#47763

Purpose

mammalian expression of rat PMCA3b

Depositor

John Penniston, Emanuel Strehler

Publication

Enyedi et al J Biol Chem. 1997 Oct 31;272(44):27525-8

Insert

PMCA3b (Atp2b3 Rat)

Expression

Mammalian

Promoter

SV40

Available since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only
pAcGP67A-hBAI3-HormR-GAIN

Plasmid

#37848

Depositor

Axel Brunger

Publication

Arac et al EMBO J. 2012 Feb 14;31(6):1364-78. doi:

Insert

BAI3 (BAI3 Human)

Use

Baculovirus transfer vectors

Tags

8xHIS

Mutation

HormR and GAIN domains only (aa498–868

Promoter

Polyhedrin

Available since

July 6, 2012

Availability

Academic Institutions and Nonprofits only
FUW-M2-PAK2 S20A

Plasmid

#31664

Depositor

Anne Brunet

Publication

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 S20A (PAK2 Human)

Use

Lentiviral

Tags

M2

Expression

Mammalian

Mutation

Changed Serine 20 to Alanine

Available since

April 12, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG-hHIF1 alpha-IGd2

Plasmid

#22535

Purpose

To be used for the RNAi screening purposes (not for HDAC4 expression); see paper

Depositor

Connie Cepko

Publication

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

hypoxia inducible factor 1 alpha (HIF1A Human)

Expression

Mammalian

Available since

Nov. 9, 2009

Availability

Academic Institutions and Nonprofits only