166,289 results

1. pET21a-SerRS-His

   Plasmid

   #124118

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Seryl-tRNA ligase (serS Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET264-pUC 24xMS2V6 Loxp KANr Loxp

   Plasmid

   #104393

   Purpose

   Yeast endogenous mRNA tagging

   Depositor

   Robert Singer, Evelina Tutucci

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   24xMS2V6
   

   Expression

   Bacterial

   Mutation

   All Loops are U-variants, interspaced by 50 nucle…

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV phSyn1(S)-tdTomato-WPRE

   Plasmid

   #51506

   Purpose

   Can be used to generate AAV virus that will express tdTomato in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Has Service

   AAV5

   Insert

   tdTomato
   

   Use

   AAV

   Promoter

   phSyn1

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-DIO-hM4D(Gi)-mCherry

   Plasmid

   #50461

   Purpose

   Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoter

   Depositor

   Bryan Roth

   Insert

   hM4D(Gi)-mCherry (CHRM4 Human)
   

   Use

   AAV

   Tags

   HA and mCherry

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   EF1a

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-HA-TCF1

   Plasmid

   #40620

   Depositor

   Kai Ge

   Article

   Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

   Insert

   TCF7 (TCF7 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPRx-Sth1Cas9-L5

   Plasmid

   #140993

   Purpose

   Sth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in Mycobacteria

   Depositor

   Wilbert Bitter

   Article

   Meijers et al Tuberculosis (Edinb). 2020 Sep;124:10198

   Inserts

   Sth1Cas9
   TetR
   L5 attP
   L5 Int
   KanR
   

   Use

   Unspecified; Integrative in mycobactreia

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a-GlnRS-His

   Plasmid

   #124108

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Glutaminyl-tRNA ligase (glnS Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDXinit

   Plasmid

   #110101

   Purpose

   E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phages

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBUE411-GGB

   Plasmid

   #198233

   Purpose

   Wheat GRF4-GIF1 chimera and pPLTP::BBM cassette in pBUE411

   Depositor

   Andrea Gallavotti

   Inserts

   GRF4-GIF1 chimera cassette
   pPLTP::BBM cassette
   

   Use

   CRISPR; Plant binary vector

   Expression

   Plant

   Promoter

   Ubiquitin and maize PLTP

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET16b-His-ArgRS

    Plasmid

    #124104

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Argin+I4 ligase (argS Escherichia coli)
    

    Tags

    10x-His

    Expression

    Bacterial

    Available Since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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