164,577 results

1. 5` CC: Puro – loxP – GFP-C

   Plasmid

   #219563

   Purpose

   5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.

   Depositor

   Andrea Ventura

   Article

   Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

   Insert

   hPGK-promoter_PuroR_2A_loxP_GFP-C
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   hPGK

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYN4+

   Plasmid

   #78715

   Purpose

   mammalian expression of NHE1

   Depositor

   Larry Fliegel

   Article

   Reddy et al J Biol Chem. 2008 Aug 8;283(32):22018-30

   Insert

   NHE1 (SLC9A1 Human)
   

   Use

   Tags

   HA3x

   Expression

   Mammalian

   Mutation

   D720G

   Promoter

   RSV-LTR

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR221_SLC16A3

   Plasmid

   #131899

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC16A3 (SLC16A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 11, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pLJM1-B-GRAM-H-NeoR

   Plasmid

   #211709

   Purpose

   Lentiviral expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H)

   Depositor

   Yasunori Saheki

   Article

   Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

   Insert

   B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
   

   Use

   Lentiviral

   Tags

   ddFP-B3

   Expression

   Mammalian

   Mutation

   Changed Glycine 187 to Leucine

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLIX403_UBAP2L_mCherry

   Plasmid

   #105286

   Purpose

   puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein

   Depositor

   Eugene Yeo

   Article

   Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

   Insert

   UBAP2L (UBAP2L Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTwist-CMV-HDGFL2_Cryptic_TwinstrepTEV_6xHis

   Plasmid

   #232344

   Purpose

   Expresses human HDGFL2 protein with TDP-43 regulated cryptic exon, along with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag

   Depositor

   Philip Wong

   Article

   Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10

   Insert

   HDGF Like 2 (HDGFL2 Human)
   

   Use

   Tags

   GGGS linker, 6xHis and Twin-strep, TEV protease s…

   Expression

   Mammalian

   Mutation

   TDP-43 regulated cryptic exon

   Promoter

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTwist-CMV-HDGFL2_WT_TwinstrepTEV_6xHis

   Plasmid

   #232343

   Purpose

   Expresses wild-type human HDGFL2 protein with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag

   Depositor

   Philip Wong

   Article

   Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10

   Insert

   HDGF like 2 (HDGFL2 Human)
   

   Use

   Tags

   GGGS linker, 6xHis and Twin-strep, TEV protease s…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HBV 1.3-mer X-null replicon

   Plasmid

   #65461

   Purpose

   containing 1.3 units of the X-null HBV genome (subtype ayw)

   Depositor

   Wang-Shick Ryu

   Article

   Hussain et al J Gen Virol. 2009 Sep;90(Pt 9):2272-81.

   Insert

   1.3 units of the X-null HBV genome
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AsdCas12a KRAB

   Plasmid

   #188502

   Purpose

   Expresses FLAG tagged AsdCas12a KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D908A

   Promoter

   EF1a

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TFORF0626

    Plasmid

    #142279

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CEBPZ (CEBPZ Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge