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pENTR D-TOPO-C3_38

Plasmid

#60279

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ACSL1 enhancer (ACSL1 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_33

Plasmid

#60275

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

GLIS3 enhancer (GLIS3 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

Plasmid

#52419

Purpose

Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

ERAS (ERAS Human)

Promoter

CAGGS

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pNTK Gatad2a flox

Plasmid

#110812

Purpose

Donor targeting vector for generating mouse Gatad2a conditional knockout allele

Depositor

Jacob Hanna

Article

Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

Insert

mGataD2a (Gatad2a Mouse)

Use

Unspecified

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA_FRT_SNCA-Avi_HygR-2A-BirA.dna(TO_deleted). (pEHA1448)

Plasmid

#209083

Purpose

SNCA (Avi tagged) expression along with BirA ligase

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

SNCA (SNCA Human)
BirA

Tags

Avi

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C3_13

Plasmid

#60298

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

EDN3 enhancer (EDN3 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
MADR pDonor TagBFP2-3XFlag (cyto) WPRE

Plasmid

#129421

Purpose

MADR donor plasmid for FlpO+Cre-mediated insertion of a TagBFP2-3XFlag cytoplasmic reporter protein

Depositor

Joshua Breunig

Article

Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

Insert

TagBFP2-3Flag

Use

Mosaic analysis for dual recombinase-mediated cas…

Tags

3X Flag epitope tag

Promoter

none (3X polyA to mitigate episomal expression)

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLBCX-Large-T-antigenK1 mutant

Plasmid

#189978

Purpose

Retroviral vector used to immortalize human cells

Depositor

William Kaelin

Article

Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

Insert

Large-T-antigenK1 mutant

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C3_20

Plasmid

#60303

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

CDKN1C enhancer (CDKN1C Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_33

Plasmid

#60312

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

GLIS3 enhancer (GLIS3 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_ZKSCAN1_Tau10-12K317M

Plasmid

#194164

Purpose

Insert contains intronic ZKSCAN1 Alu repeat elements flanked by tau cDNA exons 10-12 with FTLD-Tau K317M mutation. Expresses the tau circRNA 12-->10 K317M with 3X flag tag in exon 10.

Depositor

Stefan Stamm

Article

Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

Insert

Microtubule-associated protein tau 10-12 WT (MAPT Human)

Tags

3X Flag

Expression

Mammalian

Mutation

Changed Lysine 317 to Methionine

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pT2iU6Apowriter2N(#355)

Plasmid

#184106

Purpose

cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis

Depositor

Sophie Vriz

Article

Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

Insert

myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2

Use

Zebrafish transient transgenesis + in vitro trans…

Tags

internal tag: 5myc

Mutation

DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

Promoter

Zf-Ubi+SP6

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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px330 Gatad2a sgRNA (for deletion)

Plasmid

#110814

Purpose

Expressed sgRNA for generating Gatad2a deletion (knockout ) in mouse cell lines

Depositor

Jacob Hanna

Article

Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

Insert

mGataD2a (Gatad2a Mouse)

Expression

Mammalian

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C3_27

Plasmid

#60307

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

C16orf80 enhancer (CFAP20 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_28

Plasmid

#60308

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

CRY2 enhancer (CRY2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_32

Plasmid

#60311

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

DGKB enhancer (DGKB Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_36

Plasmid

#60314

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

DNMT3A enhancer (DNMT3A Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_37

Plasmid

#60315

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ARHGAP26 enhancer (ARHGAP26 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_38

Plasmid

#60316

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ACSL1 enhancer (ACSL1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_39

Plasmid

#60317

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

PCSK1 enhancer (PCSK1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only