We narrowed to 10,108 results for: Coli
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Plasmid#169133PurposeE. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
Tags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speGpromoter_PA4114
Plasmid#220474Purposeheterologous expression of PA4114 under endogenous speG E. coli promoterDepositorInsertPA4114
ExpressionBacterialMutationnoneAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speGpromoter_PA1472
Plasmid#220475Purposeheterologous expression of PA1472 under endogenous speG E. coli promoterDepositorInsertPA1472
ExpressionBacterialMutationnoneAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speGpromoter_PA1377
Plasmid#220476Purposeheterologous expression of PA1377 under endogenous speG E. coli promoterDepositorInsertPA1377
ExpressionBacterialMutationnoneAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSD118A
Plasmid#218140PurposeExpresses AviTag-6xHis-MBP-TEV-SD0(ZF2-3) for protein purification from E. coliDepositorInsertSD0_ZF2-3
TagsAviTag-6xHis-MBP-TEV-(GGGGS)2Available SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
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NylB-SCY_pET29b
Plasmid#215415PurposeExpression construct for NylB-SCY gene, codon optimized for expression in E. coliDepositorInsertNylB-SCY
ExpressionBacterialMutationR187S/F264C/D370YAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-GST-TEV-NAP1
Plasmid#217610PurposeFor expression in E. coli of NAP1DepositorAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-GST-TEV-GFP-NAP1
Plasmid#208870PurposeFor expression in E. coli of GFP-NAP1DepositorAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mScarlet-I-eDHFR-ORP5(ΔPH)
Plasmid#214277PurposeMammalian expression of ORP5(ΔPH) fused to mScarlet-I-tagged E. coli DHFRDepositorAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-AncSZ
Plasmid#214235PurposeBacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylationDepositorInsertSyk kinase (SYK Human)
Tags6xHisExpressionBacterialMutationSequence gained through ancestral reconstruction …PromoterT7Available SinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
p11d-RPA70/14 (MSW#134)
Plasmid#208076PurposeExpression of human Replication Protein A 70 and 14-kDa subunits in E. coli with 14 having a His-tagDepositorAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G)
Plasmid#180029PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G) (PDCD6IP Human)
TagsGB1-His6-TEVExpressionBacterialMutationP801GAvailable SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-MBP-DQNAT
Plasmid#198215PurposepJL1 plasmid encoding E. coli maltose binding protein (MBP) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tagDepositorInsertMBP_DQNAT
Tags6x His Tag and DQNAT glycosylation tagExpressionBacterialPromoterT7Available SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70DA7-DadR/DadS-Pdadh-DadhABC(A2)
Plasmid#191630PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain with transcriptional regulators DadR/DadSDepositorInsertDopamine dehydroxylase
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadHR)-DadR-Pdadh-DadhABC(A2)
Plasmid#191645PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain with transcriptional regulator DadRDepositorInsertDopamine dehydroxylase
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.6RT4-Pct5.1-crRNA(hcdR)-RT(ΔhcdR)
Plasmid#191646PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(hcdR-targeting spacer) hcdR-deleting repair template, used for hcdR deletionDepositorInsertCymR
UseCRISPRExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP3-C93A-C96A_rpoA-U
Plasmid#190962PurposeTest the U-to-C editing activity of KP3:C93A/C96A on AtrpoA editing site, in E. coliDepositorInsertPLS-KP3
TagsHisExpressionBacterialMutationDYW-KP:C93A/C96APromoterT7Available SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6-C93A-C96A_rpoA-C
Plasmid#190970PurposeTest the C-to-U editing activity of KP6:C93A/C96A on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
TagsHisExpressionBacterialMutationDYW-KP:C93A/C96APromoterT7Available SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP2-C93A-C96A_rpoA-U
Plasmid#190958PurposeTest the U-to-C editing activity of KP2:C93A/C96A on AtrpoA editing site, in E. coliDepositorInsertPLS-KP2
TagsHisExpressionBacterialMutationDYW-KP:C93A/C96APromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only