164,577 results

1. R620-M11-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6

   Plasmid

   #170195

   Purpose

   Mammalian expression of SARS-CoV-2 soluble spike trimer protein

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) (S SARS-CoV-2)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available Since

   Nov. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiTet-LDLR-mCherry BlastR

   Plasmid

   #186739

   Purpose

   Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   LDLR (LDLR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST26-MSRA-C-HA

   Plasmid

   #195177

   Purpose

   Vector for transient expression of MSRA gene with C-terminal HA tag, gene flanked by att sites for gateway cloning

   Depositor

   Stephanie Roessler

   Insert

   MSRA (MSRA Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CoxVIIIx2-OeNL(Ca2+)_18μ-pcDNA3

   Plasmid

   #111924

   Purpose

   Orange color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of OeNL(Ca2+)_18μ

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   OeNL(Ca2+)_18μ
   

   Use

   Tags

   CoxVIIIx2

   Expression

   Mammalian

   Mutation

   E67D, E104D, D133E at CaM

   Promoter

   CMV

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. FH-TET1-pEF

   Plasmid

   #49792

   Purpose

   This plasmid expresses the full-length human TET1 gene with a Flag and HA tag.

   Depositor

   Anjana Rao

   Article

   Tahiliani et al Science. 2009 May 15;324(5929):930-5. Ep

   Insert

   Tet methylcytosine dioxygenase 1 (TET1 Human)
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223_PIK3CB_WT

   Plasmid

   #82221

   Purpose

   Gateway Donor vector containing PIK3CB , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   PIK3CB (PIK3CB Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRE-Tn7-167

   Plasmid

   #118579

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mClover3
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

   Plasmid

   #67979

   Purpose

   Cas9 activity reporter (control) with BFP and GFP.

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFH3.3_Cre-MS2x12

   Plasmid

   #205559

   Purpose

   Express Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   Cre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. IDG_CACNB2_OE_1

    Plasmid

    #161684

    Purpose

    Overexpresses CACNB2

    Depositor

    Mike McManus

    Insert

    CACNB2 (CACNB2 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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