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pX330-PITCh-CEP192

Plasmid

#227288

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CEP192 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of CEP192 (CEP192 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-rTetR(SE)-EED-2A-mTurquoise

Plasmid

#225692

Purpose

Lentiviral expression of rTetR(SE) fused to EED and mTurquoise-NLS

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

rTetR-EED (EED Synthetic, Human)

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mTurquoise

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-DDX1-ts1

Plasmid

#174232

Purpose

DDX1 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

DDX1 shRNA (DDX1 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-DDX1-ts2

Plasmid

#174233

Purpose

DDX1 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

DDX1 shRNA (DDX1 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-DDX1-ts3

Plasmid

#174234

Purpose

DDX1 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

DDX1 shRNA (DDX1 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-IRSp53 P428L

Plasmid

#221321

Purpose

Dox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 (Baiap2 Mouse)

Use

Retroviral

Tags

3xFLAG

Mutation

P428L

Promoter

TRE3G

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-FKBP-IRSp53(pLxIS)

Plasmid

#221336

Purpose

Overexpression of IRSp53 pLxIS domain in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 pLxIS domain (Baiap2 Mouse)

Tags

6xHis-4xFKBP

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-KRAS-G121C/G13A-nick sgRNA

Plasmid

#214099

Purpose

Lentiviral vector expressing nicking sgRNA to induce KRAS G12C or G13A mutation using PE3

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

KRAS G12C or G13A nicking sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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GPR62-DuET

Plasmid

#213288

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

GPR62 (GPR62 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF0700

Plasmid

#143725

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PAXBP1 (PAXBP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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