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csd6_13-330_W158A/pET28b(+) N-tag

Plasmid

#71358

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:W158A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Tryptophan 158 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF4D03

Plasmid

#70969

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf12 (Taf12 Fly)

Use

Gateway entry vector

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_CUS1F03

Plasmid

#71071

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Su(var)205 (Su(var)205 Fly)

Use

Gateway entry vector

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL4D-no stop

Plasmid

#67337

Purpose

hArl4D without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Use

Gateway entry vector

Expression

Bacterial

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARF4-no stop

Plasmid

#67311

Purpose

hArf4 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Use

Gateway entry vector

Expression

Bacterial

Mutation

V53A, L123P and M134I mutations were unintended P…

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pENTR1A DHFR.dn-cHSF1

Plasmid

#134726

Purpose

Gateway entry vector for constitutively active dominant-negative HSF1 fused to the DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

DHFR.dn-cHSF1 (HSF1 Human)

Use

Entry vector for gateway cloning

Tags

DHFR

Promoter

None

Availability

Academic Institutions and Nonprofits only

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pCF159_BRL

Plasmid

#225962

Purpose

CMV-Intron-BRL (env protein). Expresses BRL (BaEVRLess) env for VLP production.

Depositor

Christof Fellmann

Article

Ramadoss et al Nat Commun. 2025 Nov 17;16(1):9883. doi:

Insert

CMV-Intron-BRL (env protein)

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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Flag_HsIRE1a_pBabePuro

Plasmid

#54337

Purpose

N-terminally FLAG tagged human IRE1a in retroviral pBabe puro vector

Depositor

David Ron

Article

Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

Insert

IRE1a (ERN1 Human)

Use

Retroviral

Tags

FLAG and preprotrypsin signal sequence

Expression

Mammalian

Mutation

The endogenous IRE1a signal sequence (aa1-18) was…

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pKK-BiFC-Venus

Plasmid

#105804

Purpose

Concomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

VN173 (I152L); VC155

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKK-FLAG-BirA-TEV

Plasmid

#105770

Purpose

Expression of your protein of interest in fusion with FLAG-BirA. FLAG-tagged at the N-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

FLAG-BirA-TEV

Expression

Mammalian

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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