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mCherry-FKBP-PIP4K2B

Plasmid

#202734

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A (3-108):[GGSA]4GG:PIP4K2B (PIP4K2B, FKBP1A Human)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-KPNA2

Plasmid

#192306

Purpose

Gateway entry vector for an inducible 3xFLAG-KPNA2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KPNA2 (KPNA2 Human)

Use

Gateway Cloning

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pJRoC30_VP5H

Plasmid

#188061

Purpose

PROSS-designed versatile peroxidase 5H

Depositor

Sarel Fleishman

Article

Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

Insert

PROSS-designed versatile peroxidase from Pleurotus ostreatus

Tags

S. cerevisiae native α-factor prepro-leader signa…

Expression

Yeast

Mutation

43 PROSS mutations, described in publication

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-mHP1gamma

Plasmid

#181902

Purpose

Fluorescently tagged HP1gamma

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1gamma (Cbx3 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET-Sac-Aβ(M1-42/C18C33)

Plasmid

#173759

Purpose

Expresses the AβC18C33 peptide.

Depositor

James Nowick

Article

Zhang et al Biochemistry. 2022 Feb 15;61(4):252-264.

Insert

Aβ(M1-42/C18C33) (APP Human)

Expression

Bacterial

Mutation

changed valine 18 to cysteine; changed glycine 33…

Promoter

T7

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-CTRLg2 (CG510)

Plasmid

#139456

Purpose

Lentiviral vector with non-targeting gRNA and puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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TRRAP sgRNA3

Plasmid

#138184

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRRAP

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

TRRAP (TRRAP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pFGL1079

Plasmid

#116897

Purpose

CenpC:eGFP (centromere marker)

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Inserts

Cenp C (partial)
eGFP
CenpC downstream region

Use

Fungal expression (in magnaporthe oryzae)

Mutation

truncated, no stop codon and without start codon

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-Scara3-IRES-BFP

Plasmid

#117265

Purpose

Retroviral overexpression of Scara3

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

Scara3 (Scara3 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

See depositor comments below.

Promoter

LTR

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-H031gp160

Plasmid

#123272

Purpose

Mammalian expression plasmid for Env from the H031 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (H031) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-CH115gp160

Plasmid

#123280

Purpose

Mammalian expression plasmid for Env from the CH115 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (CH115) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-25711gp160

Plasmid

#111841

Purpose

Mammalian expression plasmid for Env from the 25711 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (25711.2) Env

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh2007_pLenti_Syn_mChe-cdc42E7

Plasmid

#118620

Purpose

expression of mChe-tagged cdc42E7 under pSyn promoter for primary neurons

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E7 (Cdc42 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

changed cagtctg to ggcataa (667 - 673 nt in 3…

Promoter

synapsin I (rat)

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgQKI-2201

Plasmid

#115449

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgQKI-2201 (QKI Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shMBNL3-0455

Plasmid

#115463

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL3-0455 (MBNL3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgQKI-2204

Plasmid

#115450

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgQKI-2204 (QKI Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shRBFOX1-3261

Plasmid

#115458

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shRBFOX1-3261 (RBFOX1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shRBFOX1-3260

Plasmid

#115457

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shRBFOX1-3260 (RBFOX1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbr-HIS-ddFLN4-Fgß

Plasmid

#101719

Purpose

wildtype Fgß peptide with ddFLN4 fingerprint domain with N-terminal ybbr tag for covalent immobilization - resulting in weak forces

Depositor

Hermann Gaub

Article

Milles et al Science. 2018 Mar 30;359(6383):1527-1533

Insert

ddFLN4 fingerprint, Fgß peptide (FGB Human, Dictyostelium discoideum)

Tags

HIS and ybbr

Expression

Bacterial

Mutation

C18S in ddFLN4

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup93-SpeI

Plasmid

#87331

Purpose

Expression of shRNA targeting at Nup93

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup93

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

June 7, 2017

Availability

Academic Institutions and Nonprofits only

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