We narrowed to 19,336 results for: Kin

1. pBluescriptSKII+ U6-sgRNA(F+E) empty

   Plasmid

   #74707

   Purpose

   Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

   Depositor

   Eugene Yeo

   Article

   Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

   Insert

   U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. OA-1053A

   Plasmid

   #184006

   Purpose

   Expresses arrays of gRNA targeting eve promoter under U6b/c promoter

   Depositor

   Omar Akbari

   Article

   Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

   Insert

   U6b:sgRNAeve1 - U6c:sgRNAeve2
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEED-mScarlet3:ALFA

   Plasmid

   #219641

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in mScarlet3:ALFA using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mScarlet:ALFA SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C2 Chfr (1014)

   Plasmid

   #61853

   Purpose

   mammalian expresion of Chfr fused to EGFP

   Depositor

   Jonathon Pines

   Article

   Matsusaka et al J Cell Biol. 2004 Aug 16;166(4):507-16.

   Insert

   Chfr (CHFR Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCS2 Flag-JunB

   Plasmid

   #29687

   Depositor

   Edward De Robertis

   Article

   Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

   Insert

   jun B oncogene (JUNB Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. BirA-Myc_N_TAL1

   Plasmid

   #167728

   Purpose

   Expresses TAL1 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   TAL1 (TAL1 Human)
   

   Use

   Tags

   Myc-BirA

   Expression

   Mammalian

   Mutation

   no mutations

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pdCas9-NED

   Plasmid

   #109358

   Purpose

   Mammalian vector for expressing effector domains fused to dCas9.

   Depositor

   Antal Kiss

   Article

   Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   dCas9, SV40 NLS, 3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNL4-3 envFS eGFP Δvpu/env

   Plasmid

   #101345

   Purpose

   Deletion from NL4-3 nt 6054-7489 encompassing all of vpu and env until before the RRE

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   delta vpu/Env

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHM6-Q23

   Plasmid

   #40263

   Depositor

   David Rubinsztein

   Article

   Narain et al J Med Genet. 1999 Oct;36(10):739-46.

   Insert

   HTT partial exon 1 Q23 (HTT Human)
   

   Use

   Tags

   6x His and HA

   Expression

   Mammalian

   Mutation

   expanded poly-Q

   Promoter

   CMV

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. scFv-GCN4-sfGFP-TET1CD

    Plasmid

    #184439

    Purpose

    TET1 catalytic domain fused with sfGFP and scFv against GCN4

    Depositor

    Rhys Allan

    Article

    Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

    Insert

    scFv-GCN4-sfGFP-TET1CD
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLSSmOrange-mKate2

    Plasmid

    #99868

    Purpose

    Measure FRET between LSSmOrange and mKate2

    Depositor

    Marc Tramier

    Article

    Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

    Insert

    LSSmOrange
    

    Use

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR iRFP-ZtSH2

    Plasmid

    #188627

    Purpose

    Fluorescent reporter for CD3ε/ZtSH2 pYtag biosensor

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    ZtSH2
    

    Use

    Lentiviral

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-PKCA WT

    Plasmid

    #195202

    Purpose

    Mammalian expression vector for GFP tagged PKCA.

    Depositor

    Peter Parker, David Bryant

    Article

    Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

    Insert

    PRKCA (PRKCA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-fDIO-mCherryP2ACre

    Plasmid

    #161773

    Purpose

    Flp-Dependent mCherry and Cre expression

    Depositor

    Baoji Xu

    Article

    An et al Nat Commun. 2020 Apr 7;11(1):1729. doi:

    Insert

    mCherry-P2A-Cre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Rogi1-CMV-LAMB3-T2A-GFP-bGH

    Plasmid

    #179864

    Purpose

    Targets Rogi1 genomic region with HDR and expresses LAMB3-T2A-GFP

    Depositor

    George Church

    Article

    Aznauryan et al Cell Rep Methods. 2022 Jan 14;2(1):10015

    Insert

    LAMB3-T2A-GFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. ESCRT-I

    Plasmid

    #17634

    Depositor

    James Hurley

    Article

    Kostelansky et al Cell. 2006 Apr 7. 125(1):113-26.

    Insert

    Vps23(1-385) – His/Vps28(1-242) – Vps37(1-213) – Mvb12(1-101) (VPS28, STP22, SRN2 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. CRISPR_HLA_class_I_1

    Plasmid

    #164986

    Purpose

    Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA class I
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFlp-Tc

    Plasmid

    #222336

    Purpose

    flp-cl-sacB-pL1V-Lv1-TetAR-pBBR1-ELT3

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Inserts

    sacB (sacB )
    flp (flp )
    cI (cI )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    cl, flp, and sacB

    Available since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. C1(1-29)-miniTurbo-V5_pCDNA3

    Plasmid

    #107174

    Purpose

    expresses V5-tagged miniTurbo on the mammalian ER membrane

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. T7RNAP Cyslight

    Plasmid

    #205077

    Purpose

    T7RNAP protein expression

    Depositor

    Smita Patel

    Article

    Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

    Insert

    T7RNAP Cyslight (T7p07 Bacteriophage T7)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C125S, C347S, C510S, C515S, C530S, C723S

    Promoter

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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