We narrowed to 23,621 results for: promoter

1. pLEX301-ARF6-TagRFP

   Plasmid

   #162029

   Purpose

   Expression of tagged ARF WT

   Depositor

   David Bryant

   Article

   Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

   Insert

   ARF6 (ARF6 Human)
   

   Use

   Lentiviral

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV p2GM AMPK alpha2hp1 alpha1hp1

   Plasmid

   #89492

   Purpose

   shRNA against AMPK alpha2 and alpha1 in mouse/human to knock down protein expression

   Depositor

   Russell Jones

   Article

   Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

   Insert

   2 hairpins against mouse/human AMPKalpha1 and alpha2
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   U6 (The GFP-Puro is from a PGK promoter; the shRN…

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pLKO.1 puro CXCR4 siRNA-1

   Plasmid

   #12271

   Depositor

   Bob Weinberg

   Article

   Orimo et al Cell. 2005 May 6. 121(3):335-48.

   Insert

   CXCR4 siRNA (CXCR4 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiCas9-sgVRK1 #4-Blast

   Plasmid

   #199647

   Purpose

   Expresses Cas9 and sgRNA guide targeting VRK1

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A (VRK1 Human)
   

   Use

   Lentiviral

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Nickase SpCas9 - pCMV-T7-nSpCas9(H840A)-P2A-GFP (HES140)

   Plasmid

   #208976

   Purpose

   Nickase SpCas9 (H840A) with a P2A GFP and 3xFLAG, expressed from a CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   nSpCas9(H840)-BPNLS-P2A-GFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

   Plasmid

   #64217

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   hU6 promoter; BbsI sites for sgRNA
   H1 promoter; BamHI site for shRNA
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Promoter

   CBh, H1, and U6

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-CD86-SmBiT

   Plasmid

   #223618

   Purpose

   Lentiviral expression of human CD86 with SmBiT tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD86 (CD86 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   SmBiT

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP

   Plasmid

   #118594

   Purpose

   Parental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

   Depositor

   Stanley Qi

   Article

   Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

   Insert

   sgRNA/Puro-T2A-BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha

   Available Since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIRIGF-IKZF1-V2

   Plasmid

   #69045

   Purpose

   expression of IKZF1 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF1 (IKZF1 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   splice variant 2

   Promoter

   IRES

   Available Since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-VCF1 WT

    Plasmid

    #223018

    Purpose

    Expression of GFP-tagged VCF1 in mammalian cells.

    Depositor

    Niels Mailand

    Article

    Mirsanaye et al Nat Commun. 2024 Mar 19;15(1):2459. doi:

    Insert

    VCF1 (VCF1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV/TetO2

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-myr-AKT-HA

    Plasmid

    #31790

    Depositor

    Xin Chen

    Article

    Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Entry vector

    Tags

    HA and Myr

    Available Since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

12. Lenti-pHSE::Cre-mRuby3

    Plasmid

    #164136

    Purpose

    Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat induction

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    HSE promoter-Cre-T2A-mRuby3
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

    Expression

    Mammalian

    Promoter

    HSE synthetic promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCV-Neo-GFP/empty

    Plasmid

    #105505

    Purpose

    Empty backbone of MSCV-driven retroviral cDNA expression

    Depositor

    Christopher Vakoc

    Article

    Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

    Type

    Empty backbone

    Use

    Retroviral

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEJS-HZ96 AAV-SpABE8e-N-terminus_tracrRNA

    Plasmid

    #211817

    Purpose

    AAV vector expressing N-terminal of SpCas9-ABE8e and tracrRNA

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    N-terminus of SpCas9-ABE8e and tracrRNA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    chicken β-actin promoter and U6 promoter

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEJS-HZ100 AAV-SpABE8e-C_terminus-tracrRNA

    Plasmid

    #211818

    Purpose

    AAV vector expressing C-terminus of SpCas9-ABE8e with tracrRNA

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    C-terminus of SpCas9-ABE8e and tracrRNA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    chicken β-actin promoter and U6 promoter

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-UbC-tdTomato

    Plasmid

    #62516

    Purpose

    Expresses tdTomato under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures

    Depositor

    Jae Lee, Pantelis Tsoulfas

    Article

    Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

    Insert

    tdTomato
    

    Use

    AAV and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. FgH1tUTG_huMcl-1.1

    Plasmid

    #85530

    Purpose

    Inducible expression of guide RNA (huMcl-1.1) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    hu Mcl-1.1 (MCL1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    H1t

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX317 YAP1

    Plasmid

    #193683

    Purpose

    Constitutive lentiviral expression of YAP1

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAcUW51-gEgIL

    Plasmid

    #11622

    Depositor

    Pamela Bjorkman

    Article

    Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

    Insert

    HSV-1 glycoprotein E and HSV-1 glycoprotein I
    

    Tags

    His

    Expression

    Insect

    Mutation

    Dicistronic vector containing HSV-1 gE (KOS strai…

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

20. Lenti-40XUAS-Luc

    Plasmid

    #166455

    Purpose

    Luciferase reporter

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    40XUAS , Luciferase
    

    Use

    Lentiviral

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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